CY7-SE
(Synonyms: CY7-N-羟基琥珀酰胺酯,Sulfo-Cyanine7 Succinimidyl Ester) 目录号 : GC30261
CY7-SE(Cyanine7 Succinimidyl Ester)是一种由CY7衍生的荧光染料,可直接与生物分子(如蛋白质、抗体)的氨基(-NH₂)发生共价反应,无需额外偶联试剂(Ex=747nm, Em=774nm)。
Cas No.:477908-53-5
Sample solution is provided at 25 µL, 10mM.
CY7-SE (Cyanine7 Succinimidyl Ester) is a fluorescent dye derived from CY7. CY7-SE can directly react covalently with the amino groups (-NH₂) of biomolecules such as proteins and antibodies, without the need for additional coupling agents (Ex=747nm, Em=774nm). CY7 is a fundamental lipophilic cyanine dye. Due to CY7 absorption in the near-infrared region, CY7 has a very low background, CY7 is the most intense and stable long-wavelength dye. CY7 is particularly suitable for in vivo imaging in small animals as an alternative to radioactive elements. However, the molecular structure of CY7 contains a positively charged mesomeric ion structure, which requires modification through carboxyl groups or other chemical modifications to enable covalent binding with biomolecules[1]. CY7-SE, on the other hand, is derived from CY7 by introducing an active ester group (-NHS), which allows CY7-SE to directly label biomacromolecules containing amino groups, including antibodies, peptides, and liposomes[2]. For example, the ovarian cancer-related antibody OC183B2 labeled with CY7-SE can clearly visualize ovarian cancer cells in real-time near-infrared fluorescence imaging[3]. Additionally, D-mannosamine labeled with CY7-SE (Cy7-DM) can bind to the mannose receptors on the surface of tumor-associated macrophages (TAMs), enabling the observation of TAM aggregation in tumor tissues through near-infrared fluorescence imaging[4].
Reference:
[1] LI Y, ZHOU Y, YUE X, et al. Cyanine Conjugate-Based Biomedical Imaging Probes [J]. Adv Healthc Mater, 2020, 9(22): e2001327.
[2] ZHANG H, YAN C, LI H, et al. Rational Design of Near-Infrared Cyanine-Based Fluorescent Probes for Rapid In Vivo Sensing Cysteine [J]. ACS Appl Bio Mater, 2021, 4(3): 2001-2008.
[3] ZHANG C, LING X, GUO Y, et al. Evaluation of COC183B2 antibody targeting ovarian cancer by near-infrared fluorescence imaging [J]. Chin J Cancer Res, 2019, 31(4): 673-685.
[4] JIANG C, CAI H, PENG X, et al. Targeted Imaging of Tumor-Associated Macrophages by Cyanine 7-Labeled Mannose in Xenograft Tumors [J]. Mol Imaging, 2017, 16(1536012116689499.
CY7-SE(Cyanine7 Succinimidyl Ester)是一种由CY7衍生的荧光染料,可直接与生物分子(如蛋白质、抗体)的氨基(-NH₂)发生共价反应,无需额外偶联试剂(Ex=747nm, Em=774nm)。CY7是基础的脂溶性花菁染料,由于Cy7的吸收在近红外区背景非常低,是荧光强度最高、最稳定的长波长染料。特别适合于活体小动物体内成像代替放射性元素。但CY7分子结构中含有一个正电荷的介离子结构,需要通过羧基或其他化学修饰才能与生物分子共价结合[1]。CY7-SE则是在CY7的基础上引入了活性酯基团(-NHS),可直接用于标记含氨基的生物大分子,包括抗体、多肽和脂质体等[2]。CY7-SE标记的卵巢癌相关抗体OC183B2,可将卵巢癌细胞清晰的呈现在实时近红外荧光成像中[3]。使用CY7-SE标记D-甘露糖胺(Cy7-DM)与肿瘤相关巨噬细胞(TAM)表面的甘露糖受体结合,可在近红外荧光成像中观察肿瘤相关巨噬细胞在肿瘤组织中的聚集情况[4]。
标记抗体[1]:
抗体如果含有其它蛋白(例如serum albumin或gelatin等)或带氨基的缓冲液会影响标记,在标记前需纯化。
1.在1 L 0.15 M NaCl 溶液中透析anti-GST抗体(浓度为 0.5mL at 3mg/mL),常温透析4小时。
2.在4°C用新鲜的1L 0.15M NaCl 溶液再透析过夜。
3.第二天用1L 0.1M NaHCO3 (pH 8.3)透析4小时。
4.用0.22μm注射器过滤头过滤抗体溶液。
5.用0.1 M NaHCO3 稀释少量的抗体,在280nm处测其紫外吸收值计算标记抗体的总量
6.用DMSO配置CY7-SE溶液浓度为10mg/mL; 计算所需体积以得到想要的CyDye NHS 和抗体的比值(例如20:1),然后慢慢将其加入到抗体溶液中,同时在暗处常温缓慢搅拌45分钟。
7.用1L 0.15M NaCl 溶液常温避光透析4小时除去未标记上的CY7-SE。
8.在4°C用新鲜的1L 0.15M NaCl 溶液再避光透析过夜。
9.用1L of 0.01M PBS/ 0.01% 叠氮化钠溶液常温避光透析4小时, 在4°C常温避光再次透析过夜。
10.用0.22μm注射器过滤头过滤抗体溶液。
11.用0.01M PBS/0.01 % 叠氮化钠整数倍稀释标记抗体溶液,测量280nm(蛋白)和552nm(CY)处的紫外可见吸光度。
12.将CY7-SE标记的蛋白质溶解在PBS溶液中(pH=7.2-7.4)。
13.于裸鼠尾静脉注射200μL( 0.5mg/mL)。
14.记录体内荧光的成像图片,分析荧光药物的分布情况。
15.检测时激发波长700~770 nm ,发射波长790 nm。
F/P计算:
Cy7在750nm摩尔吸光系数为240600M-1cm-1;此蛋白在280nm的摩尔吸光系数为170000M-1cm-1;不同蛋白的摩尔吸光系数不一样;Cy7 染料本身在280nm 的吸收是750nm处的4%。按以下公式计算F/P值。
[Cy7] = A750/240600
[antibody] = {A280 - (0.04×A750)}/170000
F/P final= [Cy7]/[antibody]= {0.71×A750}/ {A280 - (0.04×A750)}
Reference:
[1] ZHANG C, CHENG H, DOU S, et al. Near-infrared fluorescent molecular probes with cetuximab in the in vivo fluorescence imaging for epithelial ovarian cancer [J]. J Ovarian Res, 2024, 17(1): 225.
Cas No. | 477908-53-5 | SDF | |
别名 | CY7-N-羟基琥珀酰胺酯,Sulfo-Cyanine7 Succinimidyl Ester | ||
Canonical SMILES | CC(/C(N1CCCCCC(ON2C(CCC2=O)=O)=O)=C\C=C\C=C\C=C\C3=[N+](CC)C(C=CC(S(=O)([O-])=O)=C4)=C4C3(C)C)(C)C5=C1C=CC(S(=O)(O)=O)=C5 | ||
分子式 | C39H45N3O10S2 | 分子量 | 779.92 |
溶解度 | DMSO : ≥ 41 mg/mL (52.57 mM) | 储存条件 | Store at -20°C, protect from light |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
||
Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
![]() |
1 mg | 5 mg | 10 mg |
1 mM | 1.2822 mL | 6.4109 mL | 12.8218 mL |
5 mM | 0.2564 mL | 1.2822 mL | 2.5644 mL |
10 mM | 0.1282 mL | 0.6411 mL | 1.2822 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet