Cypermethrin
(Synonyms: 氯氰菊酯) 目录号 : GC47158
A type II pyrethroid insecticide
Cas No.:52315-07-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cypermethrin is a type II pyrethroid insecticide.1,2,3,4 It prolongs opening of sodium channels resulting in membrane depolarization and a conductance block of the insect nervous system, and point mutations in sodium channels induce cypermethrin-resistance in house flies, cockroaches, and mosquitos.3 Cypermethrin (1-1.5 mg) reduces survival of ticks (H. anatolicum) in vitro in a concentration-dependent manner.2 Topical administration of cypermethrin reduces the number of ticks on infested cattle. Cypermethrin (5 and 10 ppm) reduces survival of lice (D. ovis) in sheep fleece and prevents re-infestation in contact-challenged sheep for 7 and 19 weeks, respectively.1 It induces developmental neurotoxicities in zebrafish, increasing mortality and edema and inducing body-axis curvature when used at concentrations ranging from 50 to 200 μg/L.5 Cypermethrin (15 mg/kg twice weekly for 24 weeks) also induces Parkinson's disease-like neurodegeneration in rats, decreasing locomotor activity, dopamine production, and the number of tyrosine hydroxylase positive (TH+) neurons in the substantia nigra.4
1.Hall, C.A.The efficiency of cypermethrin (NRDC 149) for the treatment and eradication of the sheep louse Damalinia ovisAust. Vet. J.54(10)471-472(1978) 2.Sajid, M.S., Iqbal, Z., Khan, M.N., et al.In vitro and in vivo efficacies of ivermectin and cypermethrin against the cattle tick Hyalomma anatolicum anatolicum (Acari: Ixodidae)Parasitol Res.105(4)1133-1138(2009) 3.Hu, Z., Du, Y., and Dong, K.A sodium channel mutation identified in Aedes aegypti selectively reduces cockroach sodium channel sensitivity to type I, but not type II pyrethroidsInsect Biochem. Mol. Biol.41(1)9-13(2011) 4.Singh, A.K., Tiwari, M.N., Prakash, O., et al.A current review of cypermethrin-induced neurotoxicity and nigrostriatal dopaminergic neurodegenerationCurr. Neuropharmacol.10(1)64-71(2012) 5.DeMicco, A., Cooper, K.R., Richardson, J.R., et al.Developmental neurotoxicity of pyrethroid insecticides in zebrafish embryosToxicol. Sci.113(1)177-186(2010)
| Cas No. | 52315-07-8 | SDF | |
| 别名 | 氯氰菊酯 | ||
| Canonical SMILES | O=C(C1C(C)(C)C1/C=C(Cl)\Cl)OC(C#N)C2=CC(OC3=CC=CC=C3)=CC=C2 | ||
| 分子式 | C22H19Cl2NO3 | 分子量 | 416.3 |
| 溶解度 | Chloroform: Slightly Soluble,Methanol: Slightly Soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4021 mL | 12.0106 mL | 24.0211 mL |
| 5 mM | 0.4804 mL | 2.4021 mL | 4.8042 mL |
| 10 mM | 0.2402 mL | 1.2011 mL | 2.4021 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Cypermethrin inhibits Leydig cell development and function in pubertal rats
Environ Toxicol 2022 May;37(5):1160-1172.PMID:35102696DOI:10.1002/tox.23473.
Cypermethrin is a broad-spectrum pyrethroid insecticide that is widely used. It may induce adverse endocrine-disrupting effects on the male reproductive system. Whether Cypermethrin can disrupt Leydig cell development and function in the late puberty remains elusive. The objective of this study was to explore the effect of Cypermethrin exposure to male rats on the development and function of Leydig cells in late puberty and explore the underlying mechanism. Thirty-six male Sprague-Dawley rats (age of 35 days) were gavaged with Cypermethrin (0, 12.5, 25, and 50 mg/kg/day) from postnatal day 35-49. Cypermethrin significantly lowered serum testosterone level while elevating serum luteinizing hormone level at a dose of 50 mg/kg, without altering serum follicle-stimulating hormone level. Cypermethrin markedly decreased CYP11A1-positive Leydig cell number at 50 mg/kg without affecting SOX9-positive Sertoli cell number. It significantly down-regulated the expression of Leydig cell genes, Lhcgr, Star, Cyp11a1, and Cyp17a1 and their proteins, while up-regulating the expression of Sertoli cell genes, Dhh and Amh, and their proteins, at doses of 12.5-50 mg/kg. In addition, Cypermethrin significantly increased malondialdehyde level while lowering the expression of Sod1 and Sod2 and their proteins at 50 mg/kg. Cypermethrin markedly induced reactive oxidative species at a concentration of 200 μM and reduced mitochondrial membrane potential at 25 μM and higher concentrations after 24 h of treatment to primary Leydig cells in vitro. In conclusion, Cypermethrin inhibits the development and function of Leydig cells in male rats in late puberty.
Cypermethrin induced toxicities in fish and adverse health outcomes: Its prevention and control measure adaptation
J Environ Manage 2018 Jan 15;206:863-871.PMID:29202434DOI:10.1016/j.jenvman.2017.11.076.
Pesticides are being widely employed in the modern agriculture, though in different quantities, across the globe. Although it is useful for crops yield enhancement, however, there are the serious environment, health and safety related concerns for aquatic and terrestrial living biomes that include humans, animals, and plants. Various in practice and emerging pesticides adversely affect the survival, development and biological systems stability. Several research efforts have been made to highlight the bio-safety and toxicological features of toxicants through risk assessment studies using different animal models, e.g., different fish species. Among several pesticides, Cypermethrin is extensively used in agriculture and households, and the reported concentrations of this pesticide in different water bodies including rivers and streams, soil and even in rainwater are threatening. Consequently, Cypermethrin is considered for risk assessment studies to know about its deep and different level of toxicological effects subject to its dose, exposure time and route. The Cypermethrin existence/persistence in the environment is posing a severe threat to humans as well as another non-target terrestrial and aquatic organism. Herein, the toxic effects of pesticides, with special reference to Cypermethrin, on fish, the mode of toxicity, concerns regarding public health and harmful impacts on human beings are comprehensively reviewed. The information is also given on their appropriate control and prevention strategies.
Cypermethrin induces apoptosis of Sertoli cells through the endoplasmic reticulum pathway
Toxicol Ind Health 2022 Jul;38(7):399-407.PMID:35610186DOI:10.1177/07482337221104905.
Cypermethrin, an extensively used pyrethroid pesticide, is regarded as one of many endocrine-disrupting chemicals (EDCs) with anti-androgenic activity to damage male reproductive systems. We previously found cypermethrin-induced apoptosis in mouse Sertoli cells TM4. We hypothesized cypermethrin-induced TM4 apoptosis by the endoplasmic reticulum (ER) pathway. This study aimed to explore the roles of the ER pathway in cypermethrin-induced apoptosis in TM4 cells. The cells were treated with Cypermethrin for 24 h at various concentrations (0 µM, 10 µM, 20 µM, 40 µM, and 80 µM). Flow cytometry was used to test for apoptosis. Western blot was used to test protein expressions in the ER stress pathway. The results showed that the apoptosis rate of TM4 cells increased with increased concentrations of Cypermethrin, and a significant difference was detected in the 80-μM group. The protein expressions of glucose-regulated protein 78 (GRP78), protein kinase R (PKR)-like ER kinase (PERK), p-PERK, α subunit of eukaryotic initiation factor (eIF2α), p-eIF2α, activating transcription factor 4 (ATF4), C/EBP homologous protein (CHOP), caspase-12, caspase-9, and caspase-3 increased with increased concentrations of Cypermethrin. The results suggested cypermethrin-induced apoptosis in TM4 cells regulated by the ER pathway involving PERK-eIF2α-ATF4-CHOP. The study provides a new insight into cypermethrin-induced apoptosis in Sertoli cells.
Ototoxicity of Cypermethrin in Wistar rats
Braz J Otorhinolaryngol 2020 Sep-Oct;86(5):587-592.PMID:31122882DOI:10.1016/j.bjorl.2019.02.007.
Introduction: This study presents the effect of Cypermethrin on the cochlear function in Wistar rats post-subchronic inhalation exposure. Worldwide several pesticides are described as causing health disorders. Cypermethrin is currently one of the most commonly used, however, little is known about its harmful effects, especially related to hearing. Human studies have associated pesticides with hearing disorders, but they present limited conclusions due to the multiple factors to which the population is exposed, such as noise. Objective: Mimic human exposure to Cypermethrin and to verify the effects on cochlear function. Methods: It is a subchronic inhalation animal study (6 weeks, 4hours/day), using 36 male Wistar aged 60 day. Rats were randomly assigned into three groups: Control (12 rats exposed to inhalation of water); Positive Control for auditory lesion (12 rats administrated with 24mg/kg intraperitoneal cisplatin); Experimental (12 rats exposed to inhalation of Cypermethrin - 0.25mg/L). Animals were evaluated by distortion product otoacoustic emissions pre- and post-exposure. Results: The frequencies of 8, 10 and 12kHz in both ears (right p=0.003; 0.004; 0.008 and left 0.003; 0.016; 0.005 respectively) and at frequencies 4 and 6 in the right ear (p=0.007 and 0.015, respectively) in the animals exposed to Cypermethrin resulted in reduction. Conclusion: Subchronic inhalation exposure to Cypermethrin provided ototoxicity in rats.
Do Cypermethrin and fenvalerate disturb the function of the bovine cervix in vitro?
J Endocrinol 2022 May 6;253(3):133-142.PMID:35343441DOI:10.1530/JOE-21-0336.
This study aimed to investigate the effect of pyrethroid insecticides on the regulation of bovine cervical function. Cervical cells or strips obtained from cows during the periovulation period were treated with Cypermethrin and fenvalerate (0.1-10 ng/mL). None of the pyrethroids exerted a cytotoxic effect, whereas only fenvalerate increased the cervical contraction force and mRNA expression of receptor of oxytocin and prostaglandin (PG) synthases. Both pyrethroids inhibited PG secretion and decreased the amount of diacylglycerol, which is the second messenger involved in oxytocin signal transmission, and fenvalerate decreased the myosin light-chain kinase level. These findings indicate that fenvalerate induces greater disruption of cervical function than Cypermethrin.