Cytarabine 5′-monophosphate
(Synonyms: ara-CMP, 1-β-D-Arabinofuranosylcytosine 5'-monophosphate, Cytosine Arabinoside monophosphate, NSC 99445) 目录号 : GC49863An active metabolite of cytarabine
Cas No.:7075-11-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cytarabine 5′-monophosphate (ara-CMP) is an active metabolite of the nucleoside analog cytarabine .1 It is formed from cytarabine by deoxycytidine kinase.2 ara-CMP is incorporated into DNA by DNA polymerase α, which slows the rate of DNA synthesis by approximately 2,000-fold in a cell-free assay.3 It inhibits nuclear and mitochondrial DNA replication in S. cerevisiae when used at a concentration of 15 mM.4 ara-CMP (3.5-75.1 mg/kg) improves survival in an L1210 murine leukemia model.5
1.Huang, S., Liu, L., Liu, X., et al.Development and application of a rapid and sensitive liquid chromatography-mass spectrometry method for simultaneous analysis of cytarabine, cytarabine monophosphate, cytarabine diphosphate and cytarabine triphosphate in the cytosol and nucleusJ. Pharm. Biomed. Anal.211114582(2022) 2.Momparler, R.L., and Fischer, G.A.Mammalian deoxynucleoside kinases. I. Deoxycytidine kinase: Purification, properties, and kinetic studies with cytosine arabinosideJ. Biol. Chem.243(16)4298-4304(1968) 3.Perrino, F.W., and Mekosh, H.L.Incorporation of cytosine arabinoside monophosphate into DNA at internucleotide linkages by human DNA polymerase αJ. Biol. Chem.267(32)23043-23051(1992) 4.Mcintosh, E.M., Kunz, B.A., and Haynes, R.H.Inhibition of DNA replication in Saccharomyces cerevisiae by araCMPCurr. Genet.10(8)579-585(1986) 5.Schrecker, A.W., and Goldin, A.Antitumor effect and mode of action of 1-β-D-arabinofuranosylcytosine 5’-phosphate in leukemia L1210Cancer Res.28(4)802-803(1968)
| Cas No. | 7075-11-8 | SDF | Download SDF |
| 别名 | ara-CMP, 1-β-D-Arabinofuranosylcytosine 5'-monophosphate, Cytosine Arabinoside monophosphate, NSC 99445 | ||
| Canonical SMILES | OP(OC[C@@H]1[C@@H](O)[C@H](O)[C@]([H])(N2C(N=C(N)C=C2)=O)O1)(O)=O | ||
| 分子式 | C9H14N3O8P | 分子量 | 323.2 |
| 溶解度 | Water: soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0941 mL | 15.4703 mL | 30.9406 mL |
| 5 mM | 0.6188 mL | 3.0941 mL | 6.1881 mL |
| 10 mM | 0.3094 mL | 1.547 mL | 3.0941 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。