Cytochrome C (from pig)
(Synonyms: 细胞色素C) 目录号 : GC18346
细胞色素c (from pig)是一种“极端多功能”蛋白，属于c型细胞色素家族的1类，介导呼吸链中的电子转移，并作为解毒剂处理ROS，在细胞凋亡中发挥作用。
Cas No.:9007-43-6
Sample solution is provided at 25 µL, 10mM.
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Cytochrome c (from pig), a 'extreme multifunctional' protein that belongs to class 1 of the c-type cytochrome family, mediates electron-transfer in the respiratory chain and acts as a detoxifying agent to dispose of ROS and plays a role in cell apoptosis[1].
Cyt c isolated from cow heart tissue is phosphorylated on tyrosine 97 in vivo, which leads to inhibition of respiration in the reaction with CcO (cytochrome c oxidase)[2].
In vivo phosphorylated Cyt c (Cytochrome c) shows enhanced sigmoidal kinetics with COX(cytochrome c oxidase), and half-maximal turnover is observed at a Cyt c substrate concentration of 5.5 microM compared to 2.5 microM for alkaline phosphatase-treated Cyt c[3].
References:
[1] Santucci R, et al. Cytochrome c: An extreme multifunctional protein with a key role in cell fate. Int J Biol Macromol. 2019 Sep 1;136:1237-1246.
[2] Yu H, et al. Mammalian liver cytochrome c is tyrosine-48 phosphorylated in vivo, inhibiting mitochondrial respiration. Biochim Biophys Acta. 2008 Jul-Aug;1777(7-8):1066-71.
[3] Lee I, et al. New prospects for an old enzyme: mammalian cytochrome c is tyrosine-phosphorylated in vivo. Biochemistry. 2006 Aug 1;45(30):9121-8.
细胞色素c (from pig)是一种“极端多功能”蛋白，属于c型细胞色素家族的1类，介导呼吸链中的电子转移，并作为解毒剂处理ROS，在细胞凋亡中发挥作用[1]。
从奶牛心脏组织中分离的Cyt c在体内被酪氨酸97磷酸化，这导致与CcO（细胞色素c氧化酶）反应中的呼吸抑制[2]。
体内磷酸化的Cyt c（细胞色素c）显示出与COX（细胞色素c氧化酶）增强的S型动力学，并且在5.5μM的Cyt c底物浓度下观察到一半最大周转，而碱性磷酸酶处理的Cyt c为2.5μM[3]。
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Cell experiment [1]: |
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Cell lines |
THP-1 cells and HEK 293T cell |
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Preparation method |
Cell lysates were prepared from LPS stimulated differentiated THP-1 cells and HEK 293T cell expressing HA-NEK7 or not. The cell lysates were mixed and incubated with or without cytochrome c (0.25 mg/ml) for 30 minutes at 30°C. The status of full length and cleaved caspase-9 was assessed by immunoblotting. |
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Reaction Conditions |
0.25 mg/ml; 30 minutes at 30°C |
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Applications |
In the absence of cytochrome c an increase in cleaved caspase-1 in NEK7 overexpressed cells, while co-expression of cytochrome c inhibited the increase. |
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Animal experiment [2]: |
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Animal models |
Sprague-Dawley rats |
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Preparation method |
Thirty Sprague-Dawley rats were randomly divided into the vehicle control group, PMB group, PMB + cyto c (Cytochrome c) 50, 100, or 200 mg/kg group, respectively, and were treated with intravenous cyto c 30 min before the administration of PMB 4.0 mg/kg once a day for consecutive 5 days. On the 4th day after administration, 24 h urine was collected to determine N-acetyl-β-D-glucosaminidase excretion. Six hours after the last injection on the 5th day, kidneys were harvested to assay PMB concentration and observe pathological alterations, and blood samples were collected to assay serum creatinine (SCr), blood urea nitrogen (BUN), and blood β2-microglobulin (β2-MG) levels. |
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Dosage form |
50, 100, or 200 mg/kg; i.v. |
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Applications |
Cyto c 50, 100, and 200 mg/kg decreased the accumulation of PMB in the kidney by 18.5%, 39.1%, and 36.8%, respectively, and reduced 24 h N-acetyl-β-D- glucosaminidase excretion by 22.5% , 40.4% , and 40.4%, respectively. Kidney pathological damage induced by PMB was markedly reduced by cyto c 100 mg/kg and 200 mg/kg. |
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References: [1] Shi CS, Kehrl JH. Cytochrome c Negatively Regulates NLRP3 Inflammasomes. PLoS One. 2016 Dec 28;11(12):e0167636. [2] Li ZD, et al. Cytochrome C suppresses renal accumulation and nephrotoxicity of polymyxin B. Hum Exp Toxicol. 2019 Feb;38(2):193-200. |
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| Cas No. | 9007-43-6 | SDF | |
| 别名 | 细胞色素C | ||
| 分子式 | C42H54FeN8O6S2 | 分子量 | 12000~13000 |
| 溶解度 | H2O:10 mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.0833 mL | 0.4167 mL | 0.8333 mL |
| 5 mM | 0.0167 mL | 0.0833 mL | 0.1667 mL |
| 10 mM | 0.0083 mL | 0.0417 mL | 0.0833 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet