CytoTrace™ Orange CMTMR
(Synonyms: CYTOTRACE橙色荧光示踪探针CMTMR,CellTracker? Orange CMTMR) 目录号 : GC43362A cell-permeant, vital fluorescent dye
Cas No.:323192-14-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >90.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
CytoTrace™ Orange CMTMR is a rhodamine-based, cell-permeant, vital fluorescent dye that is retained by cells for prolonged periods (>72 hours) and is passed to daughter cells. Cells loaded with long-lasting dyes, including CMTMR, are used to follow cell trafficking and cell-to-cell interactions in vivo.[1],[2],[3]They have also been used to estimate the growth of implanted tumor spheroids.[4] CytoTrace™ Orange CMTMR displays excitation/emission maxima of 541/565 nm, respectively.
Reference:
[1]. Brooks, A.E.S., Parsamand, T., Kelly, R.W., et al. An improved quantitative method to assess adhesive properties of Trichomonas vaginalis to host vaginal ectocervical cells using flow cytometry. J. Microbiol. Methods 92(1), 73-78 (2013).
[2]. Buehler, C., Dong, C.Y., So, P.T.C., et al. Time-resolved polarization imaging by pump-probe (stimulated emission) fluorescence microscopy. Biophys J. 79(1), 536-549 (2000).
[3]. Sato, K., Imai, Y., and Irimura, T. Contribution of dermal macrophage trafficking in the sensitization phase of contact hypersensitivity. J. Immunol. 161(12), 6835-6844 (1998).
[4]. Borgström, P., Hillan, K.J., Sriramarao, P., et al. Complete inhibition of angiogenesis and growth of microtumors by anti-vascular endothelial growth factor neutralizing antibody: Novel concepts of angiostatic therapy from intravital videomicroscopy. Cancer Res. 56(17), 4032-4039 (1996).
| Cas No. | 323192-14-9 | SDF | |
| 别名 | CYTOTRACE橙色荧光示踪探针CMTMR,CellTracker? Orange CMTMR | ||
| 化学名 | 9-[2-carboxy-4(or 5)-[[4-(chloromethyl)benzoyl]amino]phenyl]-3,6-bis(dimethylamino)-xanthylium, inner salt | ||
| Canonical SMILES | CN(C)C(C=C1)=CC2=C1C(C3=CC=C(NC(C4=CC=C(CCl)C=C4)=O)C=C3C([O-])=O)=C(C=CC(N(C)C)=C5)C5=[O+]2.CN(C)C(C=C6)=CC7=C6C(C8=CC(NC(C9=CC=C(CCl)C=C9)=O)=CC=C8C([O-])=O)=C(C=CC(N(C)C)=C%10)C%10=[O+]7 | ||
| 分子式 | C32H28ClN3O4 | 分子量 | 554 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8051 mL | 9.0253 mL | 18.0505 mL |
| 5 mM | 0.361 mL | 1.8051 mL | 3.6101 mL |
| 10 mM | 0.1805 mL | 0.9025 mL | 1.8051 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。