CytoTrace Red CMTPX
目录号 : GC43364CytoTrace Red CMTPX is a fluorogenic dye used as a long-term cell tracer.
Cas No.:942416-35-5
Sample solution is provided at 25 µL, 10mM.
CytoTrace Red CMTPX is a fluorogenic dye used as a long-term cell tracer. It is nonfluorescent but cell-permeant and, upon entering the cell, is converted to a fluorescent compound in the cytosol. CytoTrace Red CMTPX is retained by living cells and displays excitation/emission spectra of 577/602 nm, respectively. It remains fluorescent for at least 72 hours and is retained through cell division for several cycles.
NOTE: The following is a recommended protocol and only provides a guideline. lt should be modified according to specific lab needs.
1. Prepare a 2-10 mM DMSO stock solution.
NOTE: The stock solution should be used promptly; any remaining solution should be aliquoted and frozen at -20°C. Avoid repeated freeze-thaw cycles and protect from light.
2. Prepare a dye working solution.
Prepare a 1-20 μM dye working solution immediately before use by diluting the DMSO stock solution from Step 1 with Hanks' and 20 mM HEPES buffer (HHBS), or the buffer of your choice, at pH 7.0. Mix well by vortexing.
3. Analyze cells with a flow cytometer or a fluorescence microscope:
a. Treat cells with test compounds for a desired period of time.
b. Centrifuge cells to obtain 2-10 x 105 cells per tube.
c. Resuspend cells in 500 ul of the dye working solution (from Step 2).
d. Incubate cells with dye solution at room temperature or 37°C for 15 to 30 minutes, protected from light.
e. Remove the dye working solution from the cells and wash the cells with HHBS or buffer of your choice.
f. Resuspend cells in 500 ul pre-warmed HHBS or medium to get 2-10 x 105 cells per tube.
g. Monitor the fluorescence at excitation and emission wavelengths of 577 and 602 nm, respectively, with a flow cytometer (FL1 channel) or a fluorescence microscope.
NOTE for bacterial cell staining: Staining is most efficient when the stock dye solution is diluted 1:800 in nutrient broth preconditioned by overnight growth of the test bacteria, but fresh nutrient broth or PBs may also be used. Bacterial suspensions should be diluted with PBS to 105-107 organisms per ml. Bacteria may be stained by applying one ml of solution to a 0.45 μm filter (25 mm, vacuum filtering to remove the solution, then adding 1 ml of dye solution and incubating 5 to 10 minutes at room temperature.
Cas No. | 942416-35-5 | SDF | |
化学名 | 4(or 5)-(4-(chloromethyl)benzamido)-2-(1,2,2,4,8,10,10,11-octamethyl-1,2,10,11-tetrahydropyrano[3,2-g:5,6-g']diquinolin-13-ium-6-yl)benzoate | ||
Canonical SMILES | CN(C(C)(C)C=C1C)C(C1=C2)=CC3=C2C(C4=CC=C(NC(C5=CC=C(CCl)C=C5)=O)C=C4C([O-])=O)=C(C=C6C(N(C)C(C)(C)C=C6C)=C7)C7=[O+]3.CN(C(C)(C)C=C8C)C(C8=C9)=CC%10=C9C(C%11=CC(NC(C%12=CC=C(CCl)C=C%12)=O)=CC=C%11C([O-])=O)=C(C=C%13C(N(C)C(C)(C)C=C%13C)=C%14)C%14=[O+] | ||
分子式 | C42H40ClN3O4 | 分子量 | 686.2 |
溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
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1 mg | 5 mg | 10 mg |
1 mM | 1.4573 mL | 7.2865 mL | 14.573 mL |
5 mM | 0.2915 mL | 1.4573 mL | 2.9146 mL |
10 mM | 0.1457 mL | 0.7287 mL | 1.4573 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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