Danshensu (Dan shen suan A)
(Synonyms: 丹参素; Dan shen suan A; Salvianic acid A) 目录号 : GC34010
丹参素是丹参的一种活性成分，通过激活 Nrf2 信号通路显示出广泛的心血管益处。
Cas No.:76822-21-4
Sample solution is provided at 25 µL, 10mM.
Danshensu, an active ingredient of Salvia miltiorrhiza, shows wide cardiovascular benefit by activating Nrf2 signaling pathway.
Danshensu (DSS) significantly decreases the level of the marker enzymes (creatine kinase and lactate dehydrogenase) from the coronary effluents and myocardial infarction size. This could markedly contribute to the recovery of cardiac function after I/R injury. DSS also has ROS scavenging activity and boosts endogenous antioxidants such as SOD, CAT, MDA, GSH-PX and HO-1 activities by activating nuclear factor erythroid-2-related factor 2 (Nrf2) signaling pathway which is mediated by Akt and ERK1/2 in western blot analysis[2].
Acute treatment with a single dose of danshensu in rats with normal tHcy does not change plasma tHcy. In contrast, danshensu significantly lowers tHcy in rats with elevated tHcy. The relatively higher cysteine and glutathione levels after treatment with danshensu indicates that its tHcy-lowering effect is via increased activity of the trans-sulphuration pathway[1].
[1]. YG Cao, et al. Beneficial effects of danshensu, an active component of Salvia miltiorrhiza, on homocysteine metabolism via the trans-sulphuration pathway in rats. Br J Pharmacol. 2009 Jun; 157(3): 482-490. [2]. Yu J, et al. Danshensu protects isolated heart against ischemia reperfusion injury through activation of Akt/ERK1/2/Nrf2 signaling. Int J Clin Exp Med. 2015 Sep 15;8(9):14793-804.
丹参素是丹参的一种活性成分,通过激活 Nrf2 信号通路显示出广泛的心血管益处。
丹参素 (DSS) 显着降低冠状动脉流出物中标记酶（肌酸激酶和乳酸脱氢酶）的水平和心肌梗塞面积。这可能显着有助于 I/R 损伤后心脏功能的恢复。 DSS 还具有 ROS 清除活性,并通过激活由 Akt 和 ERK1/ 2 在蛋白质印迹分析中[2]。
用单剂量丹参素对 tHcy 正常的大鼠进行急性治疗不会改变血浆 tHcy。相反,丹参素显着降低了 tHcy 升高的大鼠的 tHcy。丹参素治疗后相对较高的半胱氨酸和谷胱甘肽水平表明其降低tHcy的作用是通过增加转硫途径的活性[1]。
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Animal experiment: |
All chemicals are dissolved in saline, except for tolcapone which is dissolved in saline containing 20% (v/v) PEG 200. During experiments, rats are fasted overnight and randomLy assigned into different groups. About 200 µL blood is taken from orbital sinus alternatively after ethyl ether anaesthesia, and then the eye is quickly sterilized with alcohol and pressed with cotton. Blood samples are immediately collected into a polypropylene tube containing heparin-Na and centrifuged at 5000 g at 5°C for 3 min. Prepared plasma samples are kept at −20°C and analysed within 48 h. |
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References: [1]. YG Cao, et al. Beneficial effects of danshensu, an active component of Salvia miltiorrhiza, on homocysteine metabolism via the trans-sulphuration pathway in rats. Br J Pharmacol. 2009 Jun; 157(3): 482–490. |
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| Cas No. | 76822-21-4 | SDF | |
| 别名 | 丹参素; Dan shen suan A; Salvianic acid A | ||
| Canonical SMILES | OC1=C(O)C=CC(C[C@@H](O)C(O)=O)=C1 | ||
| 分子式 | C9H10O5 | 分子量 | 198.17 |
| 溶解度 | Water : 6.2 mg/mL (31.29 mM) | 储存条件 | Store at 4°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.0462 mL | 25.2309 mL | 50.4617 mL |
| 5 mM | 1.0092 mL | 5.0462 mL | 10.0923 mL |
| 10 mM | 0.5046 mL | 2.5231 mL | 5.0462 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet