Daphnin
(Synonyms: 祖师麻乙素) 目录号 : GC63597
Daphnin 是主要香豆素生物活性成分之一，具有抗菌活性。Daphnin 是从 Daphne odora 中分离出来的。Daphne odora 是一种缓解发烧的中药。
Cas No.:486-55-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Daphnin is one of the major coumarin bioactive components with antibacterial activity. Daphnin is isolated from the whole herb of Daphne odora (Thunb.), which is a folk medicine in China for the relief of fever[1][2].
[1]. Zhu J, et al. Investigation the interaction of Daphnin with human serum albumin using optical spectroscopy and molecular modeling methods. Spectrochim Acta A Mol Biomol Spectrosc. 2012 Sep;95:252-7.
[2]. Shakeel-U-Rehman, et al. Isolation, characterisation and antibacterial activity studies of coumarins from Rhododendron lepidotum Wall. ex G. Don, Ericaceae. Rev. bras. farmacogn. vol.20 no.6 Curitiba Dec. 2010 Epub Nov 12, 2010.
| Cas No. | 486-55-5 | SDF | |
| 别名 | 祖师麻乙素 | ||
| 分子式 | C15H16O9 | 分子量 | 340.28 |
| 溶解度 | DMSO : 33.33 mg/mL (97.95 mM; Need ultrasonic) | 储存条件 | -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9388 mL | 14.6938 mL | 29.3876 mL |
| 5 mM | 0.5878 mL | 2.9388 mL | 5.8775 mL |
| 10 mM | 0.2939 mL | 1.4694 mL | 2.9388 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Investigation the interaction of Daphnin with human serum albumin using optical spectroscopy and molecular modeling methods
Spectrochim Acta A Mol Biomol Spectrosc 2012 Sep;95:252-7.PMID:22634418DOI:10.1016/j.saa.2012.04.099.
The interaction between Daphnin with human serum albumin has been studied for the first time by spectroscopic methods including fluorescence quenching technology, circular dichroism (CD) spectroscopy and Fourier transform infrared (FT-IR) spectroscopy under simulative physiological conditions. The results of fluorescence titration revealed that Daphnin can quench the intrinsic fluorescence of HSA by static quenching and there is a single class of binding site on HSA. In addition, the studies of CD spectroscopy and FT-IR spectroscopy showed that the protein secondary structure changed with increases of α-helices at the drug to protein molar ratio of 2. Furthermore, the thermodynamic functions ΔH(0) and ΔS(0) for the reaction were calculated to be 11.626 kJ mol(-1) and 118.843 J mol(-1)K(-1) according to Van't Hoff equation. The thermodynamic parameters (ΔH(0) and ΔS(0)) and the molecular modeling study indicated that hydrophobic force played an important role to stabilize the Daphnin-HSA complex, and Daphnin could bind within the subdomain IIA of the HSA.
Ethylene Induction of Non-Enzymatic Metabolic Antioxidants in Matricaria chamomilla
Molecules 2020 Dec 3;25(23):5720.PMID:33287420DOI:10.3390/molecules25235720.
Phytochemical investigations of Matricaria chamomilla L. (Asteraceae) stated the presence of several compounds with an established therapeutic and antioxidant potential. The chamomile non-enzymatic antioxidant system includes low molecular mass compounds, mainly polyphenols such as cinnamic, hydroxybenzoic and chlorogenic acids, flavonoids and coumarins. The objective of this work was to evaluate the role of the non-enzymatic antioxidant system after stimulation by ethylene in tetraploid chamomile plants. Seven days of ethylene treatment significantly increased the activity of phenylalanine ammonia-lyase, which influenced the biosynthesis of protective polyphenols in the first step of their biosynthetic pathway. Subsequently, considerable enhanced levels of phenolic metabolites with a substantial antioxidant effect (syringic, vanillic and caffeic acid, 1,5-dicaffeoylquinic acid, quercetin, luteolin, Daphnin, and herniarin) were determined by HPLC-DAD-MS. The minimal information on the chlorogenic acids function in chamomile led to the isolation and identification of 5-O-feruloylquinic acid. It is accumulated during normal conditions, but after the excessive effect of abiotic stress, its level significantly decreases and levels of other caffeoylquinic acids enhance. Our results suggest that ethephon may act as a stimulant of the production of pharmaceutically important non-enzymatic antioxidants in chamomile leaves and thus, lead to an overall change in phytochemical content and therapeutic effects of chamomile plants, as well.
Coumarin glucosides from Cruciata taurica
Phytochemistry 2002 Feb;59(4):447-50.PMID:11830165DOI:10.1016/s0031-9422(01)00471-x.
Two new coumarin glycosides (1 and 2) along with two known coumarin glucosides, Daphnin (3) and daphnetin glucoside (4) were isolated from the aerial parts of Cruciata taurica. The structures of the new compounds were elucidated by spectral methods and chemical means as 7-O-(6' -acetoxy-beta-D-glucopyranosyl)-8-hydroxycoumarin (1) and 7-O-[6 '-O-(3'',4''-dihydroxycinnamoyl)-beta-D-glucopyranosyl]-8-hydroxycoumarin (2). The phylogenetic significance of coumarins in C. taurica was discussed.
COUMARINS FROM DAPHNE AXILLIFLORA (KEISSL.) POBED. AND THE ANATOMICAL CHARACTERISTICS OF ITS LEAVES AND STEMS
Georgian Med News 2021 Mar;(312):145-149.PMID:33964843doi
The aim of the study was to determine the stable diagnostic traits of a Caucasian endemic plant Daphne axilliflora (Keissl.) Pobed. and study its phytochemical composition. The following stable diagnostic characteristic were identified during the microstructural analysis: leaf surface glabrous, hypostomatic, dorsoventral; epidermal cells chaotic; curved with curved walls on both the upper and lower epidermis; stomata anomocytic; well visible chloroplasts, rhomboid and needle shaped crystals in lower leaf epidermis; leaf vascular bundles reverse-collateral; vascular system monocyclic, bilateral; wood diffuse-porous; xylem parenchyma is apotracheal, scanty metatracheal; vessel walls are predominantly characterized by spiral and pitted thickening; collenchyma lamellar; radial rays in single rows, heterogeneous. Phytochemical analysis showed the presence of terpene and phenolic substances, including coumarins. Two well-known coumarins (Daphnin and daphnetin) were isolated and identified.
Coumarins of Matricaria chamomilla L.: aglycones and glycosides
Food Chem 2013 Nov 1;141(1):54-9.PMID:23768326DOI:10.1016/j.foodchem.2013.03.004.
The identity and quantity of coumarin-like compounds in leaves and anthodia of Matricaria chamomilla L. were studied by LC-DAD and NMR. So far, two monosubstituted coumarins, herniarin and umbelliferone, and two herniarin precursors were identified therein. In this paper, two other coumarin glycosides and one aglycone were confirmed. Skimmin (umbelliferone-7-O-β-d-glucoside), Daphnin (daphnetin-7-O-β-d-glucoside) and daphnetin (7,8-dihydroxycoumarin) were found for the first time in diploid and tetraploid leaves and anthodia of M. chamomilla L. Daphnetin is known as a strong sensitizer, so this compound and its glycosidic derivative can contribute to the allergic potential of chamomile. Commercial chamomile preparations were tested for their presence.