Defensin HNP-2 human
目录号 : GC66368
An antimicrobial peptide
Cas No.:99287-07-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Defensin HNP-2 is a peptide with antimicrobial properties that is secreted by human polymorphonuclear leukocytes (PMNs).1 It induces 26.2 and 43.5% lysis of mammalian cells at concentrations of 25 and 100 μg/ml, respectively.2 It also completely inhibits secretion of the exotoxin superantigen TSS toxin-1 (TSST-1) from S. aureus at a concentration of 5 ng/ml and inhibits infection of McCoy cells by C. trachomatis elementary bodies (EBs).3,4 Defensin HNP-2 binds to recombinant HIV-1 envelope glycoprotein gp120 and human CD4 (Kds = 8 and 15.8 nM, respectively).5 It also induces permeabilization of large unilamellar vesicles (LUVs) formed from 1-palmitoyl-2-oleoyl-sn-glycero-3-PG .6
1.Lehrer, R.I.Primate defensinsNat. Rev. Microbiol.2(9)727-738(2004) 2.Lichtenstein, A., Ganz, T., Selsted, M.E., et al.In vitro tumor cell cytolysis mediated by peptide defensins of human and rabbit granulocytesBlood68(6)1407-1410(1986) 3.Merriman, J.A., Nemeth, K.A., and Schlievert, P.M.Novel antimicrobial peptides that inhibit gram positive bacterial exotoxin synthesisPLoS One9(4)(2014) 4.Yasin, B., Harwig, S.S.L., Lehrer, R.I., et al.Susceptibility of Chlamydia trachomatis to protegrins and defensinsInfect. Immun.64(3)709-713(1996) 5.Wang, W., Owen, S.M., Rudolph, D.L., et al.Activity of ɑ- and θ-defensins against primary isolates of HIV-1J. Immunol.173(1)515-520(2004) 6.Wimley, W.C., Selsted, M.E., and White, S.H.Interactions between human defensins and lipid bilayers: Evidence for formation of multimeric poresProtein Sci.3(9)1362-1373(1994)
| Cas No. | 99287-07-7 | SDF | Download SDF |
| 分子式 | C147H217N43O37S6 | 分子量 | 3370.95 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.2967 mL | 1.4833 mL | 2.9665 mL |
| 5 mM | 0.0593 mL | 0.2967 mL | 0.5933 mL |
| 10 mM | 0.0297 mL | 0.1483 mL | 0.2967 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。