Denopamine
(Synonyms: (R)-(-)-Denopamine; TA-064) 目录号 : GC65955
Denopamine ((R)-(-)-Denopamine) 是一种有效的，具有口服活性的的选择性 β1-肾上腺素能 (β1-adrenergic) 激动剂。Denopamine 可延长病毒性心肌炎诱发的充血性心力衰竭小鼠模型的存活时间:抑制心脏中肿瘤坏死因子-α 的产生。具有心血管效应。
Cas No.:71771-90-9
Sample solution is provided at 25 µL, 10mM.
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Denopamine ((R)-(-)-Denopamine) is an orally active, selective β1-adrenergic agonist. Denopamine prolongs survival in a murine model of congestive heart failure induced by viral myocarditis: suppression of tumor necrosis factor-α production in the heart. Cardiovascular effects[1].
Denopamine (0.1-100 μM) suppresses LPS-induced TNF-α production in a concentration-dependent manner[1].
Cell Viability Assay[1]
| Cell Line: | Murine spleen cells |
| Concentration: | 0, 0.1, 1, 10, 100 μM |
| Incubation Time: | 5 hours |
| Result: | Decreased TNF-α levels by 96.9±6.7%, 62.7±6.5%, 53.2±8.8%, and 40.3±1.5% at 0.1, 1, 10 and 100 μmol/L, respectively. |
Denopamine (14 μmol/kg per day; oral administration; for 14 days) significantly improves the survival of the animals, attenuates myocardial lesions, and suppresses TNF-α production in vivo[1].
The plasma concentration of Denopamine is 13.1±1.9 nmol/L at 1 h, 4.3±0.9 nmol/L at 2 h, 1.8±0.5 nmol/L at 3 h, and <0.6 nmol/L at 5 h after its administration. A single 14 μmol/kg dose of denopamine in mice produces a peak level at 1 h[1].
| Animal Model: | Four-week-old inbred male DBA/2 mice[1] |
| Dosage: | 14 μmol/kg per day |
| Administration: | Oral administration; 14 days |
| Result: | Treatment significantly improved the survival of the animals (14 of 25 (56%) treated, vs 5 of 25 (20%) control mice). At day 14, the survival rate of 57.1% (16 of 28 mice) in the treated group was significantly higher than the 33.3% (10 of 30 mice) survival rate in the control group. The survival rate from day 6 to day 14 was also significantly improved in the treated group (69.6%; 16 of 23 mice) versus the control group (45.5%; 10 of 22 mice, p < 0.05). |
| Cas No. | 71771-90-9 | SDF | Download SDF |
| 别名 | (R)-(-)-Denopamine; TA-064 | ||
| 分子式 | C18H23NO4 | 分子量 | 317.38 |
| 溶解度 | DMSO : 5 mg/mL (15.75 mM; ultrasonic and warming and heat to 60°C) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1508 mL | 15.754 mL | 31.508 mL |
| 5 mM | 0.6302 mL | 3.1508 mL | 6.3016 mL |
| 10 mM | 0.3151 mL | 1.5754 mL | 3.1508 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet