Deoxynivalenol
(Synonyms: 脱氧雪腐镰刀菌烯醇) 目录号 : GC12969
脱氧雪腐镰刀菌烯醇 (DON) 是禾谷镰刀菌产生的一种高毒性次级代谢产物。
Cas No.:51481-10-8
Sample solution is provided at 25 µL, 10mM.
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Deoxynivalenol (DON) is a high-toxicity secondary metabolite produced by Fusarium graminearum. It is one of the most common mycotoxins in grains, foods and feeds.[1] deoxynivalenol, as an inhibitor of protein synthesis and as an immunomodulator, can reduce feed intake and mass gain in pigs.[3]
In vitro experiment it shown that treatment with 5 μM deoxynivalenol for 8 h in Pig jejunal cells has the time and dose dependent toxicity responses. In the meanwhile, pig jejunal cells also indicated reduced total cell counts and increased lactate dehydrogenase release after 48 h DON exposure with 0–10 μM. In IPEC-J2 cells, treatment with 20 μM deoxynivalenol at 4, 8, 12 and 24 h also significantly decreased the TEER (trans-epithelial electrical resistance) value.[2] In vitro efficacy study showed that neither 16 μM DOM-1 nor DON concentrations up to 0.4 μM affected the viability of the cells cultivated in medium or ConA. treatment with 0.8 μM deoxynivalenol for cells cultivated in medium significantly increased the count of dead cells, which was even higher at 1.6 μM.[4]
In vivo test demonstrated that mice orally 0.5 to12.5 mg/kg body weight deoxynivalenol decreased liver IGFALS mRNA levels, while 0.1 mg/kg body weight deoxynivalenol is without effect.[3]
References:
[1].Wu S, et al. Detoxification of DON by photocatalytic degradation and quality evaluation of wheat. RSC Adv. 2019 Oct 25;9(59):34351-34358.
[2].Thapa A, et al. Deoxynivalenol and Zearalenone-Synergistic or Antagonistic Agri-Food Chain Co-Contaminants? Toxins (Basel). 2021 Aug 11;13(8):561.
[3].Pestka JJ. Deoxynivalenol-induced proinflammatory gene expression: mechanisms and pathological sequelae. Toxins (Basel). 2010 Jun;2(6):1300-17.
[4].Vatzia E, et al. Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets. Toxins (Basel). 2019 Nov 5;11(11):644.
| Cell experiment [1]: | |
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Cell lines |
PBMC |
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Preparation Method |
Cells were either cultivated in medium or stimulated and cultivated with ConA (3 µg/mL) in the presence or absence of different deoxynivalenol (DON) concentrations (0.1, 0.2, 0.4, 0.8, and 1.6 µM) and a single deepoxy-deoxynivalenol (DOM-1) concentration (16 µM) at 37 °C for four days. Harvested PBMCs were subjected to flow cytometry, gated according to light scatter properties (pseudocolor plots), and analyzed in histograms for fluorescence intensity of a live/dead dye. |
|
Reaction Conditions |
0.1, 0.2, 0.4, 0.8, and 1.6 µM, 37°C for four days |
|
Applications |
For DOM-1, this 10-fold higher concentration of the maximum concentration of deoxynivalenol (i.e., 1.6 µM) was chosen based on pilot experiments showing that a deoxynivalenol concentration of 1.6 µM completely abolished ConA-induced proliferation. |
| Animal experiment [2]: | |
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Animal models |
The experiment pigs |
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Preparation Method |
Blood samples were taken from portal and jugular catheter of deoxynivalenol-fed (4.59 mg/kg) and CON-fed (4.59 mg/kg) animals 30 min before infusion of LPS, and initial LPS level was quantified in serum |
|
Dosage form |
4.59 mg/kg, p.o. |
|
Applications |
Numerically higher LPS concentrations were found in portal serum (efferent blood stream from intestine) in comparison to jugular samples in both CON and deoxynivalenol -fed animals. |
|
References: [1].Vatzia E, et al. Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets. Toxins (Basel). 2019 Nov 5;11(11):644. [2].Kahlert S, et al. Effects of deoxynivalenol-feed contamination on circulating LPS in pigs. Innate Immun. 2019 Apr;25(3):168-175. |
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| Cas No. | 51481-10-8 | SDF | |
| 别名 | 脱氧雪腐镰刀菌烯醇 | ||
| 化学名 | (2R,2'S,3R,5R,5aR,6S,9aR)-3,6-dihydroxy-5a-(hydroxymethyl)-5,8-dimethyl-2,3,4,5,5a,6-hexahydrospiro[2,5-methanobenzo[b]oxepine-10,2'-oxiran]-7(9aH)-one | ||
| Canonical SMILES | O[C@H]1[C@H]2O[C@H](C=C(C)C3=O)[C@@]([C@@H]3O)(CO)[C@](C1)(C)[C@@]24OC4 | ||
| 分子式 | C15H20O6 | 分子量 | 296.32 |
| 溶解度 | 30 mg/mL in ethanol & DMF, 25mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.3747 mL | 16.8737 mL | 33.7473 mL |
| 5 mM | 0.6749 mL | 3.3747 mL | 6.7495 mL |
| 10 mM | 0.3375 mL | 1.6874 mL | 3.3747 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet