Desmethyl Ranolazine
(Synonyms: 雷诺嗪杂质) 目录号 : GC49409
A metabolite of ranolazine
Cas No.:172430-45-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Desmethyl ranolazine is a metabolite of the antianginal agent ranolazine .1 It is formed from ranolazine by the cytochrome P450 (CYP) isoform CYP3A.
1.Wang, Y., Chen, X., Sun, Z., et al.Development and validation of a sensitive LC-MS/MS assay for simultaneous quantitation of ranolazine and its three metabolites in human plasmaJ. Chromatogr. B889-89010-16(2012)
| Cas No. | 172430-45-4 | SDF | |
| 别名 | 雷诺嗪杂质 | ||
| Canonical SMILES | O=C(CN1CCN(CC(COC2=C(O)C=CC=C2)O)CC1)NC3=C(C)C=CC=C3C | ||
| 分子式 | C23H31N3O4 | 分子量 | 413.5 |
| 溶解度 | DMSO: soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4184 mL | 12.0919 mL | 24.1838 mL |
| 5 mM | 0.4837 mL | 2.4184 mL | 4.8368 mL |
| 10 mM | 0.2418 mL | 1.2092 mL | 2.4184 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Enantioselective analysis of ranolazine and Desmethyl Ranolazine in microsomal medium using dispersive liquid-liquid microextraction and LC-MS/MS
Bioanalysis 2013 Jan;5(2):171-83.PMID:23330560DOI:10.4155/bio.12.308.
Background: An enantioselective bioanalytical method using dispersive liquid-liquid microextraction (DLLME) and LC-MS/MS was developed for the chiral analysis of ranolazine (RNZ) and one of its metabolites (Desmethyl Ranolazine [DRNZ]). Results: The analytes were extracted from microsomal medium by DLLME, using chloroform as extractor solvent and acetone as dispersive solvent. The enantiomers of RNZ and DRNZ were analyzed simultaneously for the first time using a Chiralcel OD-H(?). Method validation showed recoveries in the order of 55 and 45%, and LLOQ of 25 and 10 ng ml(-1) for the enantiomers of RNZ and DRNZ, respectively. Linearity was established in the concentration range of 10 to 1000 and 25 to 2500 ng ml(-1) for each DRNZ and RNZ enantiomer, respectively. Conclusion: The unprecedented use of DLLME was demonstrated to be very useful for sample preparation of microsomal matrix. Furthermore, the in vitro metabolism of RNZ was enantioselective.