Desmopressin Acetate
(Synonyms: 醋酸去氨加压素,DDAVP) 目录号 : GC11265
Desmopressin Acetate（DDAVP）是加压素的合成肽类似物，是一种安全的抗利尿和止血化合物，可作为血管加压素V2膜受体（V2R）的选择性激动剂。
Cas No.:62288-83-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
Cell lines | F3II cells |
Preparation Method | F3II cells were cultured in the presence of appropriate concentrations of Desmopressin Acetate for 7 days, and the cytostatic effect of Desmopressin Acetate was examined at low cell density using a colony formation assay. |
Reaction Conditions | 0-1000 nM, 7 days |
Applications | Desmopressin Acetate can inhibit the colony formation of F3II cells with an IC50 value of 700 nM. |
| Animal experiment [2]: | |
Animal models | BALB/c inbred mice lung metastasis model |
Preparation Method | Desmopressin Acetate was co-injected at the time of intravenous injection of tumor cells at a final dose of 1 µg (F3II cells) or 2 µg (LM3 cells) per kg of mouse body weight. |
Dosage form | 1 or 2 µg/kg, once, i.v. |
Applications | Desmopressin Acetate significantly inhibited the formation of lung metastases and reduced the number of lung nodules by about 70%. |
References: | |
Desmopressin Acetate (DDAVP), a synthetic peptide analog of vasopressin, is a safe antidiuretic and hemostatic compound that acts as a selective agonist at the vasopressin V2 membrane receptor (V2R)[1].
Desmopressin Acetate has moderate antiproliferative activity, and at low cell densities Desmopressin Acetate significantly inhibited F3II cell colony formation with an IC50 value of 700 nM. Conditioned media from F3II monolayers exposed to low doses of Desmopressin Acetate (100 nM) significantly increased angiostatin formation in the presence of purified plasminogen. Following heterologous V2R expression, Desmopressin Acetate induces cyclic adenosine monophosphate (cAMP)-dependent endothelial nitric oxide synthase (eNOS) activation via Ser1177 phosphorylation[2].
Co-injection of Desmopressin Acetate (1-2 μg/kg) at the time of i.v. injection of F3II or LM3 cells significantly inhibited the formation of tumor lung metastases in a mouse lung metastasis model of breast cancer, with a reduction of approximately 70% in the number of lung nodules in both cases[3]. Dogs undergoing surgery for intervertebral disc disease had a significantly prolonged buccal mucosal bleeding time (BMBT) after aspirin administration, but preoperative intravenous administration of Desmopressin Acetate rapidly reversed the prolongation of the BMBT, and none of them experienced intraoperative bleeding complications[4].
References:
[1] Ripoll G V, Garona J, Pifano M, et al. Reduction of tumor angiogenesis induced by desmopressin in a breast cancer model[J]. Breast cancer research and treatment, 2013, 142: 9-18.
[2] Kaufmann J E, Iezzi M, Vischer U M. Desmopressin (DDAVP) induces NO production in human endothelial cells via V2 receptor-and cAMP-mediated signaling[J]. Journal of Thrombosis and Haemostasis, 2003, 1(4): 821-828.
[3] Alonso D F, Skilton G, Farías E F, et al. Antimetastatic effect of desmopressin in a mouse mammary tumor model[J]. Breast cancer research and treatment, 1999, 57: 271-275.
[4] Di Mauro F M, Holowaychuk M K. Intravenous administration of desmopressin acetate to reverse acetylsalicylic acid‐induced coagulopathy in three dogs[J]. Journal of Veterinary Emergency and Critical Care, 2013, 23(4): 455-458.
Desmopressin Acetate（DDAVP）是加压素的合成肽类似物，是一种安全的抗利尿和止血化合物，可作为血管加压素V2膜受体（V2R）的选择性激动剂[1]。
Desmopressin Acetate具有适度的抗增殖活性，在低细胞密度时Desmopressin Acetate可显著抑制F3II细胞集落形成，IC50值为700 nM。在纯化纤溶酶原存在的情况下，F3II单层细胞暴露于低剂量Desmopressin Acetate（100 nM）的条件培养基中会显著增加血管生成素的形成[1]。在异源V2R表达后，Desmopressin Acetate可通过Ser1177磷酸化诱导环磷酸腺苷（cAMP）依赖性内皮一氧化氮合酶（eNOS）激活[2]。
在静脉注射F3II或LM3细胞时共注射Desmopressin Acetate（1-2 μg/kg）显著抑制乳腺癌小鼠肺转移模型中肿瘤肺转移的形成，肺结节数量均减少约70%[3]。接受椎间盘疾病手术的狗在服用阿司匹林后颊粘膜出血时间（BMBT）明显延长，但术前静脉给予Desmopressin Acetate迅速逆转了BMBT的延长，并且均未出现术中出血并发症[4]。
| Cas No. | 62288-83-9 | SDF | |
| 别名 | 醋酸去氨加压素,DDAVP | ||
| 化学名 | DDAVP | ||
| Canonical SMILES | CC(=O)O.C1CC(N(C1)C(=O)C2CSSCCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)N2)CC(=O)N)CCC(=O)N)CC3=CC=CC=C3)CC4=CC=C(C=C4)O)C(=O)NC(CCCN=C(N)N)C(=O)NCC(=O)N | ||
| 分子式 | C48H68N14O14S2 | 分子量 | 1129.27 |
| 溶解度 | 100 mg/ml in DMSO; 100 mg/ml in Water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.8855 mL | 4.4276 mL | 8.8553 mL |
| 5 mM | 0.1771 mL | 0.8855 mL | 1.7711 mL |
| 10 mM | 0.0886 mL | 0.4428 mL | 0.8855 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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