DETA NONOate

DETA NONOate is a NO donor. It spontaneously dissociates in a pH-dependent, first-order process with a half-life of 20 hours and 56 hours at 37°C and 22-25°C, pH 7.4, respectively, to liberate 2 moles of NO per mole of parent compound.^[1],[2]

Laboratory Procedures

    For long term storage, keep DE'TA NONOate sealed under nitrogen at -80°C, It should be stable for at least one year. The crystals are sensitive to moisture and become discolored on exposure to air. Keep the vial sealed until use unles youilaboratory is equipped with a glove box with an inert atmosphere for the handling of air sensitive compounds.

    DETA NONOate dissociates to the firee amine and NO in a pH-dependent manner following first order kinetics. Alkaline solutions of NONOates (in 0.01 M NaOH) are very stable and can be stored at 0°C for 24 hours. DETANONOate is highly soluble in water and relatively concentrated solutions can be prepared for further dilution. To initiate the release of NO, add a portion of the stock akaline solution of DETA NONOate to excess bulfer of pH 7.0-7.4. The halflife of DETA NONOate is 20 hours and 56 hours at 37°C and 22-25°C, respectively, in 0.1 M phosphate buffer(pH7.4). DETA NONOate liberates 2 moles of NO per mole of parent compound.^[1],[2] The decomposition of NONOates is nearly instantaneous at pH 5.^[1]

   DETA NONOate is a nitric oxide (NO) donor. The intact DETA NONOate has a characteristic UV absorbance at 252 nm (ℇ = 7,640 M^-1cm^-1), permitting quantitation in aqueous solutions.^[1] The concentration of the basic stock solution of DETA NONOate can be measured by UV if there is any uncertainty about the condition under which it was prepared or stored.

Reference:

[1]. Hrabie, J.A., Klose, J.R., Wink, D.A., et al. New nitric oxide-releasing zwitterions derived from polyamines The Journal of Organic Chemistry 58, 1472-1476 (1993).
[2]. Keefer, L.K., Nims, R.W., Davies, K.M., et al. “NONOates” (1-substituted diazen-1-ium-1,2-diolates) as nitric oxide donors: Convenient nitric oxide dosage forms Methods in Enzymology 268, 281-293 (1996).