DiBAC4(3)
(Synonyms: 双(1,3-二巴比妥酸)-三次甲基氧烯洛尔) 目录号 : GC30140
DiBAC4(3)为电压敏感荧光染料，λex=490 nm， λem=505 nm。
Cas No.:70363-83-6
Sample solution is provided at 25 µL, 10mM.
DiBAC4(3) is a negatively charged fluorescent membrane potential indicator.1 Upon membrane depolarization, DiBAC4(3) enters the cytosol, binds to lipid membranes and intracellular proteins, and displays excitation/emission maxima of 490/516 nm, respectively.1,2 It has been used to measure the membrane potential of live or fixed mammalian and bacterial cells by flow cytometry or fluorescence microscopy.2,3,4
1.Yamada, A., Gaja, N., Ohya, S., et al.Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cellsJpn. J. Pharmacol.86(3)342-350(2001) 2.Warren, E.A.K., and Payne, C.K.Cellular binding of nanoparticles disrupts the membrane potentialRSC Adv.5(18)13660-13666(2015) 3.Klapperstück, T., Glanz, D., Klapperstück, M., et al.Methodological aspects of measuring absolute values of membrane potential in human cells by flow cytometryCytometry A.75(7)593-608(2009) 4.Mason, D.J., Allman, R., Stark, J.M., et al.Rapid estimation of bacterial antibiotic susceptibility with flow cytometryJ. Microsc.176(Pt 1)8-16(1994)
Cell experiment: | Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)[1]. |
References: [1]. Yamada A, et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn J Pharmacol. 2001 Jul;86(3):342-50. | |
| Cas No. | 70363-83-6 | SDF | |
| 别名 | 双(1,3-二巴比妥酸)-三次甲基氧烯洛尔 | ||
| Canonical SMILES | O=C1N(CCCC)C(/C(C(N1CCCC)=O)=C/C=C/C(C(N2CCCC)=O)C(N(CCCC)C2=O)=O)=O | ||
| 分子式 | C27H40N4O6 | 分子量 | 516.63 |
| 溶解度 | DMSO : ≥ 60 mg/mL (116.14 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9356 mL | 9.6781 mL | 19.3562 mL |
| 5 mM | 0.3871 mL | 1.9356 mL | 3.8712 mL |
| 10 mM | 0.1936 mL | 0.9678 mL | 1.9356 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet