Dibutyryl-cAMP, sodium salt
(Synonyms: Dibutyryl cAMP sodium salt; DBcAMP sodium salt) 目录号 : GC12824
Dibutyryl-cAMP, sodium salt，又称为Bucladesine，是一种细胞膜可透性环核苷酸类似物，能模拟内源性cAMP的作用，并作为磷酸二酯酶抑制剂。
Cas No.:16980-89-5
Sample solution is provided at 25 µL, 10mM.
Dibutyryl-cAMP, sodium salt, also known as Bucladesine, is a cell-permeable cyclic nucleotide analogue which mimics the action of endogenous cAMP and acts as a phosphodiesterase inhibitor[1]. Dibutyryl-cAMP, sodium salt is widely used to increase intracellular cAMP level[2] and is commonly employed in research on cell signaling, neurobiology, and inflammation[3].
Dibutyryl-cAMP, sodium salt (300nM; 1h) treatment can induce neuronal differentiation by up-regulating the expression of nur77, through the acetylation of Lys14 on histone H3 in PC12 cells[4]. Apoptosis induced by SGP in PC12 cells can be inhibited by 0.5mM to 1mM dibutyryl-cAMP, sodium salt[5].
Dibutyryl-cAMP, sodium salt (300nM/mouse; i.p.) pretreatment altered seizure parameters induced by pentylenetetrazol (PTZ), significantly decreasing seizure latency and threshold[6]. Dibutyryl-cAMP, sodium salt (50, 100, 300nM/mouse; i.p.) significantly decreased thermal-induced pain sensation in a dose-dependent manner in mice[7].
References:
[1]. Azami K, Etminani M, Tabrizian K, et al. The quantitative evaluation of cholinergic markers in spatial memory improvement induced by nicotine-bucladesine combination in rats. Eur J Pharmacol. 2010 Jun 25;636(1-3):102-7. doi: 10.1016/j.ejphar.2010.03.041. Epub 2010 Mar 31. PMID: 20361958.
[2]. Neumann S, Bradke F, Tessier-Lavigne M, et al. Regeneration of sensory axons within the injured spinal cord induced by intraganglionic cAMP elevation. Neuron. 2002 Jun 13;34(6):885-93. doi: 10.1016/s0896-6273(02)00702-x. PMID: 12086637.
[3]. Rundfeldt C, Steckel H, Sörensen T, Wlaź P. The stable cyclic adenosine monophosphate analogue, dibutyryl cyclo-adenosine monophosphate (bucladesine), is active in a model of acute skin inflammation. Arch Dermatol Res. 2012 May;304(4):313-7. doi: 10.1007/s00403-012-1216-6. PMID: 22302126; PMCID: PMC3332354.
[4]. Maruoka H, Sasaya H, Shimamura Y, et al. Dibutyryl-cAMP up-regulates nur77 expression via histone modification during neurite outgrowth in PC12 cells. J Biochem. 2010 Jul;148(1):93-101. doi: 10.1093/jb/mvq036. Epub 2010 Apr 7. PMID: 20375114.
[5]. Kobayashi Y, Shinozawa T. Effect of dibutyryl cAMP and several reagents on apoptosis in PC12 cells induced by a sialoglycopeptide from bovine brain. Brain Res. 1997 Dec 19;778(2):309-17. doi: 10.1016/s0006-8993(97)01072-x. PMID: 9459548.
[6]. Hosseini-Zare MS, Salehi F, Seyedi SY, et al. Effects of pentoxifylline and H-89 on epileptogenic activity of bucladesine in pentylenetetrazol-treated mice. Eur J Pharmacol. 2011 Nov 30;670(2-3):464-70. doi: 10.1016/j.ejphar.2011.09.026. Epub 2011 Sep 21. PMID: 21946102.
[7]. Salehi F, Hosseini-Zare MS, Aghajani H, Seyedi SY, Hosseini-Zare MS, Sharifzadeh M. Effect of bucladesine, pentoxifylline, and H-89 as cyclic adenosine monophosphate analog, phosphodiesterase, and protein kinase A inhibitor on acute pain. Fundam Clin Pharmacol. 2017 Aug;31(4):411-419. doi: 10.1111/fcp.12282. Epub 2017 Apr 26. PMID: 28267871.
Dibutyryl-cAMP, sodium salt，又称为Bucladesine，是一种细胞膜可透性环核苷酸类似物，能模拟内源性cAMP的作用，并作为磷酸二酯酶抑制剂[1]。Dibutyryl-cAMP, sodium salt广泛用于增加细胞内cAMP水平[2]，常用于细胞信号传导、神经生物学和炎症研究[3]。
Dibutyryl-cAMP, sodium salt (300nM; 1小时)处理可通过组蛋白H3上Lys14的乙酰化在PC12细胞中上调nur77的表达，从而诱导神经分化[4]。由SGP诱导的PC12细胞凋亡可被0.5mM至1mM的Dibutyryl-cAMP, sodium salt抑制[5]。
Dibutyryl-cAMP, sodium salt (300nM/mouse; 腹腔注射)预处理改变了戊四氮(PTZ)诱导的癫痫参数，显著减少了癫痫发作潜伏期和阈值[6]。Dibutyryl-cAMP, sodium salt (50, 100, 300nM/mouse，腹腔注射)以剂量依赖性方式显著减少了小鼠的热诱导疼痛感觉[7]。
| Cell experiment [1]: | |
Cell lines | PC12 cells |
Preparation Method | PC12 cells were treated with 300nM Dibutyryl-cAMP for 1h. The levels of histone H3 acetylation were then measured by immunoblot analysis using anti-histone H3 or anti-acetylated histone H3 antibodies. |
Reaction Conditions | 300nM; 1h |
Applications | Dibutyryl-cAMP treatment stimulates acetylation of histone H3 in PC12 cells. |
| Animal experiment [2]: | |
Animal models | Male albino mice |
Preparation Method | Dibutyryl-cAMP were administered intraperitoneally (i.p.), 30min before intravenous (i.v.) infusion of pentylenetetrazol (PTZ) (0.5% w/v). Then sizure threshold was measured. |
Dosage form | 300nM/mouse; i.p. |
Applications | Dibutyryl-cAMP treatment significantly decreased seizure latency and threshold on pentylenetetrazol (PTZ) induced seizure in mice. |
References: | |
| Cas No. | 16980-89-5 | SDF | |
| 别名 | Dibutyryl cAMP sodium salt; DBcAMP sodium salt | ||
| 化学名 | sodium (4aR,6S,7R,7aR)-6-(6-butyramido-9H-purin-9-yl)-7-(butyryloxy)tetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinin-2-olate 2-oxide | ||
| Canonical SMILES | CCCC(NC1=NC=NC2=C1N=CN2[C@H]3O[C@H]4[C@@H](OP(OC4)([O-])=O)[C@H]3OC(CCC)=O)=O.[Na+] | ||
| 分子式 | C18H23N5NaO8P | 分子量 | 491.37 |
| 溶解度 | ≥ 49.1mg/mL in Water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0351 mL | 10.1756 mL | 20.3513 mL |
| 5 mM | 0.407 mL | 2.0351 mL | 4.0703 mL |
| 10 mM | 0.2035 mL | 1.0176 mL | 2.0351 mL |
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| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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1. 首先保证母液是澄清的；
2.
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Quality Control & SDS
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- Purity: >98.00%
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