Digitonin
(Synonyms: 洋地黄皂苷) 目录号 : GC32854
洋地黄皂苷是一种甾体皂苷，广泛用作温和的非离子洗涤剂。
Cas No.:11024-24-1
Sample solution is provided at 25 µL, 10mM.
Digitonin is a steroidal saponin used extensively as a mild non-ionic detergent [1]. Digitonin finds application as a cholesterol precipitating agent [2]. Digitonin increases cell permeability by binding specifically to cholesterol in the cell membrane [4].
Digitonin had high exhibition against Caco-2, MCF-7 with IC50 of 15.72 ± 0.16 and 5.23 ± 0.61μM, respectively. Combinations of the panel of SM with a non-toxic concentration of digitonin (2 or 5 μM) generally enhanced the cytotoxicity in Caco-2, MCF-7 cells [3]. Pretreatment with digitonin increases the tumour uptake of carboplatin and potentiates the cytotoxic effect of carboplatin [4].
References:
[1]. Bridges C D. A method for preparing stable digitonin solutions for visual pigment extraction[J]. Vision Research, 1977, 17(2): 301-302.
[2]. Haust H L, Kuksis A, Beveridge J M R. Quantitative precipitation of various 3β-hydroxysterols with digitonin[J]. Canadian Journal of Biochemistry, 1966, 44(1): 119-128.
[3]. Eid S Y, El-Readi M Z, Wink M. Digitonin synergistically enhances the cytotoxicity of plant secondary metabolites in cancer cells[J]. Phytomedicine, 2012, 19(14): 1307-1314.
[4]. Lindnér P G, Heath D, Howell S B, et al. Digitonin enhances the efficacy of carboplatin in liver tumour after intra-arterial administration[J]. Cancer chemotherapy and pharmacology, 1997, 40(5): 444-448.
洋地黄皂苷是一种甾体皂苷,广泛用作温和的非离子洗涤剂[1]。洋地黄皂苷可用作胆固醇沉淀剂[2]。洋地黄皂苷通过特异性结合细胞膜中的胆固醇来增加细胞通透性[4]。
Digitonin 对 Caco-2、MCF-7 具有很强的抑制作用,IC50 分别为 15.72 ± 0.16 和 5.23 ± 0.61μM。 SM 组与无毒浓度的洋地黄皂苷（2 或 5 μM）的组合通常增强 Caco-2、MCF-7 细胞的细胞毒性[3]。毛地黄皂苷预处理可增加卡铂的肿瘤摄取并增强卡铂的细胞毒作用[4]。
| Cell experiment [1]: | |
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Cell lines |
Caco-2 and MCF-7 cells |
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Preparation Method |
The cells were incubated with 100 µl of medium containing different concentrations of digitonin and other secondary metabolites for 24 hours. |
|
Reaction Conditions |
0-500µM for 24 hours. |
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Applications |
Digitonin showed the high exhibition to Caco-2, MCF-7 with IC50 of 15.72 ± 0.16 and 5.23 ± 0.61µM, respectively. |
| Animal experiment [2]: | |
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Animal models |
Inbred rats of both sexes of the Lister-Hooded (LH) strain |
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Preparation Method |
Digitonin was dissolved in dimethylsulfoxide (DMSO) and diluted with saline to yield a solution that contained 400 µM digitonin and 40 µM DMSO; 0.2 ml of the solution was infused into the hepatic artery. |
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Dosage form |
400 µM 0.2 ml of Digitonin solution, a catheter (PE 10, inner diameter 0.28 mm, outer diameter 0.61 mm) was inserted into the hepatic artery proper via the gastroduodenal artery |
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Applications |
After pretreatment with digitonin the platinum content in the tumour as measured at 1 h after CBDCA infusion was 66±19 ng/mg. This was more than 5 times higher than the values obtained after pretreatment with saline alone (12±4 ng/mg) or with DMSO-saline (10±2 ng/mg) |
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References: [1]: Eid S Y, El-Readi M Z, Wink M. Digitonin synergistically enhances the cytotoxicity of plant secondary metabolites in cancer cells[J]. Phytomedicine, 2012, 19(14): 1307-1314. |
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| Cas No. | 11024-24-1 | SDF | |
| 别名 | 洋地黄皂苷 | ||
| Canonical SMILES | C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])[C@@H](O)[C@@]1([H])[C@@](CC[C@]5([H])[C@@]6(C[C@@H](O)[C@H](O[C@]([C@@H]([C@@H](O)[C@H]7O[C@@](O[C@H](CO)[C@@H](O)[C@@H]8O[C@@](OC[C@@H](O)[C@@H]9O)([H])[C@@H]9O)([H])[C@@H]8O[C@@](O[C@H](CO)[C@H | ||
| 分子式 | C₅₆H₉₂O₂₉ | 分子量 | 1229.31 |
| 溶解度 | DMF: 30 mg/ml, DMSO: 30 mg/ml | 储存条件 | Store at 4°C, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.8135 mL | 4.0673 mL | 8.1346 mL |
| 5 mM | 0.1627 mL | 0.8135 mL | 1.6269 mL |
| 10 mM | 0.0813 mL | 0.4067 mL | 0.8135 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >50.00%
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