Dihydrotestosterone
(Synonyms: 雄诺龙,stanolone,5α-DHT) 目录号 : GC19064二氢睾酮 (DHT) 是一种内源性雄激素类固醇和激素，可作为雄激素受体的激动剂。
Cas No.:521-18-6
Sample solution is provided at 25 µL, 10mM.
Dihydrotestosterone(DHT) is an endogenous androgenic steroid and hormone that acts as an agonist for androgen receptors.
Dihydrotestosterone regulation of miRNA expression. CD44 expression and cell adhesion to hyaluronic acid (HA) were down-regulated when cells were treated with Dihydrotestosterone or transfection with a miR-328-3p mimic[1]. Altered miRNA levels when luminal breast cancer MCF-7 cancer cancer cells were treated with Dihydrotestosterone [2]. The expression of one critical miRNA, namely let-7a-d, was also found to be up-regulated in Dihydrotestosterone-treated MDA-MB-453 cells [3]. Treatment of MDA-MB-453 cells with Dihydrotestosterone causes down-regulation of miR-125b and miR-100 in association of increased expression of matrix metalloprotease 13, a target of both miRNAs [4]. Dihydrotestosterone treatment enhanced STAT5 phosphorylation and promoted proliferation of all CRPC cells. On immunofluorescence, activation of STAT5 and GR translocating into the nucleus after Dihydrotestosterone treatment [5].
Dihydrotestosterone induced early hair regression, hair miniaturization, hair density loss, and changes in hair morphology in male C57BL/6 mice [6]. The presence of the possible negative regulation of cell proliferation by Dihydrotestosterone. Moreover, cell proliferation related to urethral tube formation was revealed to be Dihydrotestosterone dose dependent [7]. Dihydrotestosterone-treated ovariectomized mice had free access to food (free-feeding), they had increased food intake and higher body weight compared with control animals. These mice also had a significantly greater accumulation of fat in the liver and exhibited increased fasting glucose, impaired glucose tolerance, and resistance to leptin [8]. Promastigotes in the presence of Dihydrotestosterone produced significantly larger lesions in BALB/c earlobes [9].
References:
[1]. Al-Othman N, Hammad H, et,al.Dihydrotestosterone regulates expression of CD44 via miR-328-3p in triple-negative breast cancer cells. Gene. 2018 Oct 30;675:128-135. doi: 10.1016/j.gene.2018.06.094. Epub 2018 Jun 28. PMID: 29964098.
[2]. Nakano K, Miki Y, et,al. Identification of androgen-responsive microRNAs and androgen-related genes in breast cancer. Anticancer Res. 2013 Nov;33(11):4811-9. PMID: 24222117.
[3]. Lyu S, Yu Q, et,al. Androgen receptor decreases CMYC and KRAS expression by upregulating let-7a expression in ER-, PR-, AR+ breast cancer. Int J Oncol. 2014 Jan;44(1):229-37. doi: 10.3892/ijo.2013.2151. Epub 2013 Oct 25. PMID: 24172884.
[4]. Ahram M, Mustafa E, et,al.Differential expression and androgen regulation of microRNAs and metalloprotease 13 in breast cancer cells. Cell Biol Int. 2017 Dec;41(12):1345-1355. doi: 10.1002/cbin.10841. Epub 2017 Sep 5. PMID: 28816390.
[5]. Song C, Kim Y, et,al. Dihydrotestosterone enhances castration-resistant prostate cancer cell proliferation through STAT5 activation via glucocorticoid receptor pathway. Prostate. 2014 Sep;74(12):1240-8. doi: 10.1002/pros.22841. Epub 2014 Jul 7. PMID: 25043756.
[6]. Fu D, Huang J, et,al. Dihydrotestosterone-induced hair regrowth inhibition by activating androgen receptor in C57BL6 mice simulates androgenetic alopecia. Biomed Pharmacother. 2021 May;137:111247. doi: 10.1016/j.biopha.2021.111247. Epub 2021 Jan 29. PMID: 33517191.
[7]. Suzuki H, Matsushita S, et,al. 5α-Dihydrotestosterone negatively regulates cell proliferation of the periurethral ventral mesenchyme during urethral tube formation in the murine male genital tubercle. Andrology. 2017 Jan;5(1):146-152. doi: 10.1111/andr.12241. Epub 2016 Oct 1. PMID: 27696776.
[8]. Kanaya N, Vonderfecht S, et,al. Androgen (dihydrotestosterone)-mediated regulation of food intake and obesity in female mice. J Steroid Biochem Mol Biol. 2013 Nov;138:100-6. doi: 10.1016/j.jsbmb.2013.04.001. Epub 2013 May 7. PMID: 23665441; PMCID: PMC4130703.
[9]. SÁnchez-GarcÍa L, Wilkins-Rodriguez A, et,al. Dihydrotestosterone enhances growth and infectivity of Leishmania Mexicana. Parasite Immunol. 2018 Mar;40(3). doi: 10.1111/pim.12512. Epub 2018 Jan 18. PMID: 29272044.
二氢睾酮 (DHT) 是一种内源性雄激素类固醇和激素,可作为雄激素受体的激动剂。
二氢睾酮对 miRNA 表达的调节。当用二氢睾酮处理细胞或用 miR-328-3p 模拟物转染细胞时,CD44 表达和细胞与透明质酸 (HA) 的粘附下调[1]。当用二氢睾酮[2] 处理管腔乳腺癌 MCF-7 癌细胞时,miRNA 水平发生了变化。还发现一种关键 miRNA,即 let-7a-d 的表达在二氢睾酮处理的 MDA-MB-453 细胞中上调 [3]。用二氢睾酮处理 MDA-MB-453 细胞会导致 miR-125b 和 miR-100 下调,这与基质金属蛋白酶 13 的表达增加有关,基质金属蛋白酶 13 是两种 miRNA 的靶标[4]。双氢睾酮治疗增强了 STAT5 磷酸化并促进了所有 CRPC 细胞的增殖。免疫荧光显示,双氢睾酮处理后 STAT5 激活和 GR 易位入核[5]。
双氢睾酮诱导早期毛发退化、毛发小型化、毛发密度下降和毛发变化雄性 C57BL/6 小鼠的形态 [6]。二氢睾酮可能存在对细胞增殖的负调节。此外,与尿道管形成相关的细胞增殖显示为二氢睾酮剂量依赖性[7]。双氢睾酮治疗的卵巢切除小鼠可以自由进食（自由喂养）,与对照动物相比,它们的食物摄入量增加,体重增加。这些小鼠在肝脏中的脂肪堆积也明显增加,并表现出空腹血糖升高、葡萄糖耐量降低和瘦素抵抗[8]。双氢睾酮存在下的前鞭毛体在 BALB/c 耳垂中产生了明显更大的损伤 [9]。
| Cell experiment [1]: | |
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Cell lines |
MDA-MB-231 cells |
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Preparation Method |
Investigation of the regulation of miRNA expression following treatment of MDA-MB-231 cells with 100 nM Dihydrotestosterone for three days was carried out using PCR arrays. |
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Reaction Conditions |
100 nM Dihydrotestosterone for three days |
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Applications |
Dihydrotestosterone regulation of miRNA expression. CD44 expression and cell adhesion to hyaluronic acid (HA) were down-regulated when cells were treated with Dihydrotestosterone or transfection with a miR-328-3p mimic. |
| Animal experiment [2]: | |
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Animal models |
Seven-week-old male C57BL/6 mice |
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Preparation Method |
Mice were administered Dihydrotestosterone (0.5-2 mg/d, dissolved in corn oil), Dihydrotestosterone (1 mg/d body weight) plus bicalutamide (0.5 mg/d body weight), or corn oil alone (control group) two or five times per week intraperitoneally, subcutaneously, or topically. |
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Dosage form |
0.5-2 mg/d Dihydrotestosterone two or five times per week intraperitoneally, subcutaneously, or topically. |
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Applications |
Dihydrotestosterone was found to induce early hair regression, hair miniaturization, hair density loss, and changes in hair morphology in male C57BL/6 mice. |
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References: [1]. Al-Othman N, Hammad H,et,al.Dihydrotestosterone regulates expression of CD44 via miR-328-3p in triple-negative breast cancer cells. Gene. 2018 Oct 30;675:128-135. doi: 10.1016/j.gene.2018.06.094. Epub 2018 Jun 28. PMID: 29964098. |
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| Cas No. | 521-18-6 | SDF | |
| 别名 | 雄诺龙,stanolone,5α-DHT | ||
| 分子式 | C19H30O2 | 分子量 | 290.44 |
| 溶解度 | ≥100mg/mL in DMSO; 58 mg/mL in Ethanol; Water: Insoluble | 储存条件 | Store at RT, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4431 mL | 17.2153 mL | 34.4305 mL |
| 5 mM | 0.6886 mL | 3.4431 mL | 6.8861 mL |
| 10 mM | 0.3443 mL | 1.7215 mL | 3.4431 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >97.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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