DIMBOA
(Synonyms: 丁布) 目录号 : GC60138
DIMBOA(2,4-二羟基-7-甲氧基- 2h -1,4-苯并恶嗪-3(4H)- 1)是一种苯并恶嗪类化合物(BX),是禾本科植物的次生代谢物。
Cas No.:15893-52-4
Sample solution is provided at 25 µL, 10mM.
DIMBOA (2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one) is a benzoxazinoid (BX), is a secondary metabolite in grasses [1]. DIMBOA act as antibiotic and allelochemical, part of the chemical defense system of graminaceous plants such as maize, wheat, and rye [1,2]. DIMBOA possess growth inhibitory properties against many strains of studied bacteria and fungi, such as Staphylococcus aureus, Escherichia coli as well as against Saccharomyces cerevisiae [2]. Once released, BXs degrade relatively quickly in aqueous environments with a half-life of less than 24 hours [1].
DIMBOA is weak bacterial mutagens and furthermore caused aneuploidy in human-derived liver cells (HepG2 cells) [3]. DIMBOA showed genotoxic effects in HepG2 cell line [4].
DIMBOA showed the antibacterial activity against Ralstonia solanacearum with minimum inhibitory concentrations (MICs) of 50, 100 and 200 mg/L. DIMBOA also significantly affect bacterial growth, reduce biofilm formation, and inhibit swarming motility within 24 h [5].
References:
[1]. Neal A L, Ahmad S, Gordon-Weeks R, et al. Benzoxazinoids in root exudates of maize attract Pseudomonas putida to the rhizosphere[J]. PloS one, 2012, 7(4): e35498.
[2]. Gleńsk M, Gajda B, Franiczek R, et al. In vitro evaluation of the antioxidant and antimicrobial activity of DIMBOA [2, 4-dihydroxy-7-methoxy-2 H-1, 4-benzoxazin-3 (4 H)-one][J]. Natural product research, 2016, 30(11): 1305-1308.
[3]. Buchmann C A, Nersesyan A, Kopp B, et al. Dihydroxy-7-methoxy-1, 4-benzoxazin-3-one (DIMBOA) and 2, 4-dihydroxy-1, 4-benzoxazin-3-one (DIBOA), two naturally occurring benzoxazinones contained in sprouts of Gramineae are potent aneugens in human-derived liver cells (HepG2)[J]. Cancer letters, 2007, 246(1-2): 290-299.
[4]. Buchmann C, Nersesyan A, Kopp B, et al. DIMBOA and DIBOA, two naturally occuring benzoxazinoides, cause aneuploidy in a human-derived liver cell line (HepG2)[C]//BMC Pharmacology. BioMed Central, 2007, 7(2): 1-1.
[5]. Guo B, Zhang Y, Li S, et al. Extract from maize (Zea mays L.): antibacterial activity of DIMBOA and its derivatives against Ralstonia solanacearum[J]. Molecules, 2016, 21(10): 1397.
Cell experiment [1]: | |
Cell lines |
Pseudomonas putida KT2440 |
Preparation Method |
P. putida KT2440 cells were grown to mid-exponential phase in 100 mL M9 medium before DIMBOA was added to a final concentration of 5 µg mL-1. After 1 hour of exposure, cells were harvested by centrifugation at 4°C. |
Reaction Conditions |
5 µg mL-1, 1h |
Applications |
P. putida KT2440 appears relatively tolerant to DIMBOA in comparison to other soil bacteria. For all subsequent experiments, DIMBOA was employed at concentrations of 5 µg mL-1 (0.023 mM). |
References: [1]: Neal A L, Ahmad S, Gordon-Weeks R, et al. Benzoxazinoids in root exudates of maize attract Pseudomonas putida to the rhizosphere[J]. PloS one, 2012, 7(4): e35498. |
Cas No. | 15893-52-4 | SDF | |
别名 | 丁布 | ||
Canonical SMILES | O=C1C(O)OC2=CC(OC)=CC=C2N1O | ||
分子式 | C9H9NO5 | 分子量 | 211.17 |
溶解度 | DMSO: 125 mg/mL (591.94 mM) | 储存条件 | Store at -20°C |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
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1 mg | 5 mg | 10 mg |
1 mM | 4.7355 mL | 23.6776 mL | 47.3552 mL |
5 mM | 0.9471 mL | 4.7355 mL | 9.471 mL |
10 mM | 0.4736 mL | 2.3678 mL | 4.7355 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
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% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
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