Dinoprost (Prostaglandin F2a)
(Synonyms: 地诺前列素; Prostaglandin F2α; PGF2α) 目录号 : GC30370
Dinoprost (Prostaglandin F2a)是一种口服有效的强效前列腺素受体 （FP 受体） 激动剂。Dinoprost刺激子宫肌层活动，放松宫颈，抑制黄体类固醇生成，并通过直接作用诱导黄体溶解。
Cas No.:551-11-1
Sample solution is provided at 25 µL, 10mM.
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Dinoprost (Prostaglandin F2a) is an orally active, potent prostaglandin receptor (FP receptor) agonist.Dinoprost stimulates myometrial activity, relaxes the cervix, inhibits corpus luteal steroidogenesis, and induces luteolysis by direct action[1].
Dinoprost (1μM; 24h) induces ER stress, autophagy, and apoptosis in goat luteal cells[2]. Dinoprost (1μg/mL; 24h) stimulated P4 secretion, luprostiol did not in uence P4 synthesis. The cytotoxic and pro-apoptotic effects were observed in the Dinoprost-treated cells[3].
After confirming that the corpus luteum is mature in the ewe, Dinoprost (12.5 mg; im) is injected. Half an hour later, the blood flow velocity of the ewe increases, and one hour later, the NO level rises rapidly, which has a negative impact on the corpus luteum[4]. In rats treated with Dinoprost (6mg/kg; sc; 3d) after ovarian I/R injury, the expression of COX-2 was reduced, the expression of IL-1β and TNF-α did not change significantly, and the amount of bleeding was reduced [5].
References:
[1].Wen X, Liu L, Li S, et al. Prostaglandin F2α induces goat corpus luteum regression via endoplasmic reticulum stress and autophagy[J]. Frontiers in Physiology, 2020, 11: 868.
[2].Thieme H, Schimmat C, Münzer G, et al. Endothelin antagonism: effects of FP receptor agonists prostaglandin F2α and fluprostenol on trabecular meshwork contractility[J]. Investigative ophthalmology & visual science, 2006, 47(3): 938-945.
[3]. Korzekwa A J, Lukasik K, Pilawski W, et al. Influence of prostaglandin F2α analogues on the secretory function of bovine luteal cells and ovarian arterial contractility in vitro[J]. The Veterinary Journal, 2014, 199(1): 131-137.
[4].El-Sherry T M, Senosy W, Mahmoud G B, et al. Effect of dinoprost and cloprostenol on serum nitric oxide and corpus luteum blood flow during luteolysis in ewes[J]. Theriogenology, 2013, 80(5): 513-518.
[5].Karakus S, Ozkaraca M. Investigating the protective potential of dinoprost in a rat model of ischemia-reperfusion[J]. 2023.
Dinoprost (Prostaglandin F2a)是一种口服有效的强效前列腺素受体 （FP 受体） 激动剂。Dinoprost刺激子宫肌层活动，放松宫颈，抑制黄体类固醇生成，并通过直接作用诱导黄体溶解[1]。
Dinoprost（1 μM；24 h）诱导山羊黄体细胞中的 ER 应激、自噬和细胞凋亡[2]。Dinoprost（1μg/mL；24 h）刺激 P4 分泌，在用Dinoprost处理的细胞中观察到细胞毒性和促细胞凋亡作用[3]。
确认母羊体内黄体成熟后，注射Dinoprost（12.5mg；im），半小时后母羊血流速度加快，1小时后NO水平迅速升高，对黄体产生负面影响[4]。大鼠卵巢I/R损伤后经Dinoprost（6mg/kg；sc；3d）治疗，大鼠COX-2表达降低，IL-1β和TNF-α表达无明显变化，出血量减少[5]。
| Cell experiment [1]: | |
Cell lines | Bovine luteal cells |
Preparation Method | Bovine luteal cells were treated for 24 h with PGF2x (1μg/mL) ,dinoprost (1μg/mL) , luprostiol (1μg/mL), as well as luteinising hormone (positive control; 100ng/mL). |
Reaction Conditions | 1μg/mL; 24h |
Applications | Dinoprost stimulated P4 secretion ,luprostiol did not in uence P4 synthesis. The cytotoxic and pro-apoptotic effects were observed in the Dinoprost-treated cells. |
| Animal experiment [2]: | |
Animal models | Ischemia/ Reperfusion (I/R) damage model |
Preparation Method | A total of 24 rats were randomly divided into four groups:Control, Ischemia (Isch), I/R and Dino+I/R. Ischemia was induced by clamping the ovarian blood supply, followed by reperfusion. Dinoprost was administered before reperfusion in the Dino+I/R group. COX-2, IL-1β, and TNF-α expression levels were assessed through histochemical and immunochemical analyses. |
Dosage form | 6mg/kg; sc; 3d |
Applications | After Dinoprost treatment, COX-2 expression was decreased, IL-1β and TNF-α expressions did not change significantly, and the amount of bleeding was reduced. |
References: | |
| Cas No. | 551-11-1 | SDF | |
| 别名 | 地诺前列素; Prostaglandin F2α; PGF2α | ||
| Canonical SMILES | CCCCC[C@H](O)/C=C/[C@H]1[C@H](O)C[C@H](O)[C@@H]1C/C=C\CCCC(O)=O | ||
| 分子式 | C20H34O5 | 分子量 | 354.48 |
| 溶解度 | H2O : 100 mg/mL (282.10 mM; Need ultrasonic); DMSO : 100 mg/mL (282.10 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.821 mL | 14.1052 mL | 28.2103 mL |
| 5 mM | 0.5642 mL | 2.821 mL | 5.6421 mL |
| 10 mM | 0.2821 mL | 1.4105 mL | 2.821 mL |
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| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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2.
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Quality Control & SDS
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- Purity: >96.50%
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