Dipsacoside B
(Synonyms: 川续断皂苷乙；川续断皂苷 B) 目录号 : GN10730
A triterpenoid saponin with hepatoprotective activity
Cas No.:33289-85-9
Sample solution is provided at 25 µL, 10mM.
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Dipsacoside B is a major bioactive saponin, which can be used as a marker.
A liquid chromatography-electrospray ionization-mass spectrometry method has been developed and validated for identification and quantification of four major bioactive saponins in rat plasma after oral administration of extraction of saponins from Flos Lonicerae, i.e., Macranthoidin B, Macranthoidin A, Dipsacoside B, and Macranthoside B. The retention time of Macranthoidin B, Macranthoidin A, Dipsacoside B, Macranthoside B and IS are 10.65, 12.11, 13.81, 23.31 and 5.47 min, respectively[1]. Taking Macranthoidins A, B, Dipsacoside B (saponins), sweroside (iridoids), and luteolin-7-O-d-glucoside (flavonoids) as markers, a method of ultra high performance liquid chromatography with triple quadrupole mass spectrometry is employed to determine their amounts in Lonicerae Flos, Lonicerae Japonicae Flos, and Lonicerae Japonicae Flos preparations[2].
References:
[1]. Chen CY, et al. Liquid chromatography-mass spectrometry analysis of macranthoidin B, macranthoidin A, Dipsacoside B, and macranthoside B in rat plasma for the pharmacokinetic investigation. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jan 15;877(3):159-65.
[2]. Zhang X, et al. Rapid quantitative analysis of adulterant Lonicera species in preparations of Lonicerae Japonicae Flos. J Sep Sci. 2015 Dec;38(23):4014-20.
Animal experiment: | Mice[1] Sprague-Dawley rats (220-250 g) are used. To calculate the administered dose of Macranthoidin B, Macranthoidin A, Dipsacoside B, and Macranthoside B, their contents in saponins extraction are quantitatively determined. One hundred milligrams of the dry powder is suspended in 100 mL 50% methanol (v/v), ultrasonically extracted for 40 min, and then cooled at room temperature; 50% methanol is added to compensate for the lost weight. Finally, 5 μL of this solution is injected into the HPLC system for analysis after filtration through a 0.45 μm membrane. The contents of Macranthoidin B, Macranthoidin A, Dipsacoside B, and Macranthoside B are found as 36.7, 6.59, 23.3 and 0.506 g/100 g extraction, respectively. |
References: [1]. Chen CY, et al. Liquid chromatography-mass spectrometry analysis of macranthoidin B, macranthoidin A, Dipsacoside B, and macranthoside B in rat plasma for the pharmacokinetic investigation. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jan 15;877(3):159-65. | |
| Cas No. | 33289-85-9 | SDF | |
| 别名 | 川续断皂苷乙；川续断皂苷 B | ||
| 化学名 | Dipsacoside B | ||
| Canonical SMILES | CC1C(C(C(C(O1)OC2C(C(COC2OC3CCC4(C(C3(C)CO)CCC5(C4CC=C6C5(CCC7(C6CC(CC7)(C)C)C(=O)OC8C(C(C(C(O8)COC9C(C(C(C(O9)CO)O)O)O)O)O)O)C)C)C)O)O)O)O)O | ||
| 分子式 | C53H86O22 | 分子量 | 1074.56 |
| 溶解度 | ≥ 53.75mg/mL in DMSO | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.9306 mL | 4.6531 mL | 9.3061 mL |
| 5 mM | 0.1861 mL | 0.9306 mL | 1.8612 mL |
| 10 mM | 0.0931 mL | 0.4653 mL | 0.9306 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet