DLPC
(Synonyms: 1,2-二十二酰基-SN-GLYCERO-3-胆碱磷酸,DLPC) 目录号 : GC14047A phospholipid
Cas No.:18194-25-7
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
IC50: N/A
DLPC is an LRH-1 agonist ligand.
The orphan nuclear receptor liver receptor homolog-1 (LRH-1) is recognized as a critical regulator of bile salt biosynthesis. Bile salts are recognized increasingly as modulators of glucose and lipid metabolism in both human and animals.
In vitro: In a cell-free system, DLPC specifically bound to a recombinant LRH-1 ligand-binding domain. At functional level, DLPC was found to be a strong activator of both human and mouse LRH-1, while other nuclear receptors including FXR, CAR, PXR, PPARa and PPARc were all unaffected by DLPC in cell culture. In addition, DLPC induced the transactivation of the native mouse Shp and Oct4 promoters, consistent with previous studies on Lrh-1. In HepG2 cell line, DLPC could induce the expression of CYP8B1 [1].
In vivo: In mouse in vivo test, DLPC induced bile acid biosynthetic enzymes in mouse liver, lowered hepatic triglycerides and serum glucose, and increased bile acid levels. Moreover, DLPC treatment was also able to decrease hepatic steatosis and improve glucose homeostasis in two mouse models of insulin resistance [1].
Clinical trial: In a previous clinical study, single-dose inhalations of liposome preparations of Bec-DLPC and DLPC alone were administered. Results showed that no adverse clinical or laboratory events were observed. Thus, it was concluded that DLPC liposome aerosol was well tolerated in doses equivalent to those administered by MDIs for treatment of asthma [2].
References:
[1] Hohenester S,Beuers U. Phosphatidylcholines as regulators of glucose and lipid homeostasis: promises and potential risks. Hepatology.2011 Dec;54(6):2265-7.
[2] Waldrep JC,Gilbert BE,Knight CM,Black MB,Scherer PW,Knight V,Eschenbacher W. Pulmonary delivery of beclomethasone liposome aerosol in volunteers. Tolerance and safety. Chest.1997 Feb;111(2):316-23.
Cas No. | 18194-25-7 | SDF | |
别名 | 1,2-二十二酰基-SN-GLYCERO-3-胆碱磷酸,DLPC | ||
化学名 | (R)-2,3-bis(dodecanoyloxy)propyl (2-(trimethylammonio)ethyl) phosphate | ||
Canonical SMILES | O=C(CCCCCCCCCCC)O[C@@H](CO[P@](OCC[N+](C)(C)C)([O-])=O)COC(CCCCCCCCCCC)=O | ||
分子式 | C32H64NO8P | 分子量 | 621.83 |
溶解度 | 100 mg/mL in Ethanol | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.6082 mL | 8.0408 mL | 16.0816 mL |
5 mM | 0.3216 mL | 1.6082 mL | 3.2163 mL |
10 mM | 0.1608 mL | 0.8041 mL | 1.6082 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。