DOBU
(Synonyms: 2,5-Dimethoxy-4-butylamphetamine) 目录号 : GC43554
An Analytical Reference Standard
Cas No.:63779-89-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
DOBU is an analytical reference standard that is structurally categorized as an amphetamine. Experiments with isolated rat thoracic aorta suggested that this compound may act as an antagonist of the 5-HT2 receptor. This product is intended for research and forensic applications.
| Cas No. | 63779-89-5 | SDF | |
| 别名 | 2,5-Dimethoxy-4-butylamphetamine | ||
| Canonical SMILES | CC(N)CC1=CC(OC)=C(CCCC)C=C1OC | ||
| 分子式 | C15H25NO2 | 分子量 | 251.4 |
| 溶解度 | DMF: 14 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 5 mg/ml,Ethanol: 10 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9777 mL | 19.8886 mL | 39.7772 mL |
| 5 mM | 0.7955 mL | 3.9777 mL | 7.9554 mL |
| 10 mM | 0.3978 mL | 1.9889 mL | 3.9777 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Dobutamine-norepinephrine, but not vasopressin, restores the ventriculoarterial matching in experimental cardiogenic shock
Transl Res 2010 Nov;156(5):273-81.PMID:20970750DOI:10.1016/j.trsl.2010.07.011.
We assessed the hemodynamic effects of guideline therapy in experimental cardiogenic shock and compared this treatment with a combination containing an alternative vasopressor (arginine vasopressin, AVP). Our hypothesis was that combined dobutamine-norepinephrine still is the superior inopressor therapy assessed by ventriculoarterial matching in both systole and diastole. Cardiogenic shock (CS) was induced by coronary microembolization in 16 pigs. Dobutamine (DOBU, 2ug/kg/min) alone and combined with either norepinephrine (NE, 100 ng/kg/min) or the pure vasopressor AVP (0.001 u/kg/min) were infused. In CS, DOBU increased cardiac output (CO) and central venous oxygen saturation (SVO₂) from 74 ± 3 mL/kg and 37 ± 2% to 103 ± 8 mL/kg and 49 ± 3%. Adding NE resulted in a further improvement of CO (125 ± 9 mL/kg) and SVO₂ (59 ± 4%) because of an increased heart rate and contractility with minimal change in systemic vascular resistance. Also, energy transfer from the ventricle to the arterial system was restored partly by DOBU and was normalized by supplementing NE. In contrast, supplemental AVP further worsened the shock state by decreasing CO (70 ± 6 mL/kg) and SVO₂ (45 ± 5%) compared with DOBU alone. Combined Dobu-NE has an efficient hemodynamic profile in CS. A pure afterload increasing substance used in acute ischemic CS aggravates the shock state by causing a ventriculoarterial mismatch despite its use in combination with an inotropic compound.
Inward rectifier K+ channel and T-type Ca2+ channel contribute to enhancement of GABAergic transmission induced by β1-adrenoceptor in the prefrontal cortex
Exp Neurol 2017 Feb;288:51-61.PMID:27840071DOI:10.1016/j.expneurol.2016.11.007.
The functions of prefrontal cortex (PFC) are sensitive to norepinephrine (NE). Endogenously released NE influences synaptic transmission through activation of different subtypes of adrenergic receptors in PFC including α1, α2, β1 or β2-adrenoceptor. Our recent study has revealed that β1-adrenoceptor (β1-AR) activation modulates glutamatergic transmission in the PFC, whereas the roles of β1-AR in GABAergic transmission are elusive. In the current study, we probed the effects of the β1-AR agonist dobutamine (DOBU) on GABAergic transmission onto pyramidal neurons in the PFC of juvenile rats. DOBU increased both the frequency and amplitude of miniature IPSCs (mIPSCs). Ca2+ influx through T-type voltage-gated Ca2+ channel was required for Dobu-enhanced mIPSC frequency. We also found that DOBU facilitated GABA release probability and the number of releasable vesicles through regulating T-type Ca2+ channel. DOBU depolarized GABAergic fast-spiking (FS) interneurons with no effects on the firing rate of action potentials (APs) of interneurons. Dobu-induced depolarization of FS interneurons required inward rectifier K+ channel (Kir). Our results suggest that DOBU increase GABA release via inhibition of Kir, which further depolarizes FS interneurons resulting in Ca2+ influx via T-type Ca2+ channel.
Effect of lipopolysaccharide, cytokines, and catecholamines on brain natriuretic peptide release from human myocardium
Acta Anaesthesiol Scand 2012 Aug;56(7):860-5.PMID:22471594DOI:10.1111/j.1399-6576.2012.02683.x.
Background: During sepsis and septic shock, elevated plasma concentrations of brain natriuretic peptide (BNP) have been reported but may be related to several underlying mechanisms. The aim of the present experimental study was to investigate the effect of lipopolysaccharide (LPS), tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), interleukin 6 (IL-6), dobutamine (DOBU), epinephrine (Epi), and norepinephrine (Nor) on BNP synthesis by atrial human myocardium in vitro. Methods: After the approval of local ethics committee, right atrial appendages were obtained during cannulation for cardiac surgery and pinned in a isolated organ bath containing 15 ml of Tyrode's modified solution. Preparations were oxygenated, maintained at 36 ± 0.5°C and stimulated at a frequency of 1 Hz. A 60-min equilibration period was followed by 180-min exposure to 1 μM endothelin 1 (ET-1; n = 9), 20,000 pg/ml TNF-α (n = 10), 1000 pg/ml IL-1β (n = 10), 5000 pg/ml IL-6 (n = 10), 10,000 pg/ml LPS (n = 10), 100 μM Epi (n = 9), 100 μM Nor (n = 10), and 100 μM DOBU (n = 8). No product was added in Control group (n = 10). Two BNP dosages were performed: the first after 60 min of stabilization and the second after 180 min of stimulation. Absolute and relative changes in BNP concentration were compared between groups. Results: Exposure to ET-1 significantly increased BNP release as compared with Control group. DOBU, Epi, Nor, and LPS significantly increased BNP concentration but not TNF-α, IL-1β, or IL-6. Conclusions: In vitro, LPS, DOBU, Epi, and Nor induced BNP synthesis by human atrial myocardium.
Coral micro- and macro-morphological skeletal properties in response to life-long acclimatization at CO2 vents in Papua New Guinea
Sci Rep 2021 Oct 7;11(1):19927.PMID:34620911DOI:10.1038/s41598-021-98976-9.
This study investigates the effects of long-term exposure to OA on skeletal parameters of four tropical zooxanthellate corals naturally living at CO2 seeps and adjacent control sites from two locations (DOBU and Upa Upasina) in the Papua New Guinea underwater volcanic vent system. The seeps are characterized by seawater pH values ranging from 8.0 to about 7.7. The skeletal porosity of Galaxea fascicularis, Acropora millepora, massive Porites, and Pocillopora damicornis was higher (up to ~ 40%, depending on the species) at the seep sites compared to the control sites. Pocillopora damicornis also showed a decrease of micro-density (up to ~ 7%). Thus, further investigations conducted on this species showed an increase of the volume fraction of the larger pores (up to ~ 7%), a decrease of the intraskeletal organic matrix content (up to ~ 15%), and an increase of the intraskeletal water content (up to ~ 59%) at the seep sites. The organic matrix related strain and crystallite size did not vary between seep and control sites. This multi-species study showed a common phenotypic response among different zooxanthellate corals subjected to the same environmental pressures, leading to the development of a more porous skeletal phenotype under OA.
Differential effects of dobutamine, dopamine, and noradrenaline on splanchnic haemodynamics and oxygenation in the pig
Acta Anaesthesiol Scand 1995 Nov;39(8):1088-96.PMID:8607316DOI:10.1111/j.1399-6576.1995.tb04236.x.
Supranormal oxygen (O2) transport may benefit critically ill patients. Catecholamines are clinically employed for this purpose. However, their effects on splanchnic haemodynamics and oxygenation are now well defined. The effects of dobutamine (DOBU), dopamine (DOPA), and noradrenaline (NA) on splanchnic blood flows electromagnetic flow probes), O2 deliveries and uptakes (catheterisation of portal and hepatic veins) were studied in nine anaesthetised (ketamine/flunitrazepam), ventilated, paralysed, and laparotomised pigs. All three catecholamines (DOPA at 15 micrograms.kg-1.min-1, DOBU at 13 micrograms.kg-1.min-1, NA at 0.4 micrograms.kg-1.min-1) significantly (P < 0.05) increased cardiac output and systemic O2 delivery. Only DOPA increased small intestinal and total hepatic blood flows, and O2 deliveries, and decreased O2 extractions. The same parameters did not change during DOBU. During NA, total hepatic blood flow and O2 delivery decreased, and hepatic O2 extraction increased. During all three catecholamines, small intestinal and total hepatic O2 uptakes did not change significantly. Whereas hepatic arterial blood flow decreased during both DOPA and NE, portal venous flow increased during DOPA. These data suggest that in the experimental model used splanchnic O2 supply and O2 reserve capacity appear improved by DOPA, unaffected by DOBU, and impaired by NA.