Dopamine 3-O-Sulfate
(Synonyms: DA-3S, Dopamine 3-Sulfate) 目录号 : GC49166
A metabolite of dopamine
Cas No.:51317-41-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Dopamine 3-O-sulfate is a metabolite of the endogenous catecholamine dopamine .1 It is formed from dopamine by the sulfotransferase (SULT) isoform SULT1A3.2 Dopamine 3-O-sulfate is found at higher levels in the brain and circulation than dopamine 4-O-sulfate.1,2
1.Suominen, T., Uutela, P., Ketola, R.A., et al.Determination of serotonin and dopamine metabolites in human brain microdialysis and cerebrospinal fluid samples by UPLC-MS/MS: Discovery of intact glucuronide and sulfate conjugatesPLoS One8(6)e68007(2013) 2.ItÄaho, K., Alakurtti, S., Yli-Kauhaluoma, J., et al.Regioselective sulfonation of dopamine by SULT1A3 in vitro provides a molecular explanation for the preponderance of dopamine-3-O-sulfate in human blood circulationBiochem. Pharmacol.74(3)504-510(2007)
| Cas No. | 51317-41-0 | SDF | |
| 别名 | DA-3S, Dopamine 3-Sulfate | ||
| Canonical SMILES | O=S(O)(OC1=CC(CCN)=CC=C1O)=O | ||
| 分子式 | C8H11NO5S | 分子量 | 233.2 |
| 溶解度 | DMSO: slightly soluble,Methanol: slightly soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.2882 mL | 21.4408 mL | 42.8816 mL |
| 5 mM | 0.8576 mL | 4.2882 mL | 8.5763 mL |
| 10 mM | 0.4288 mL | 2.1441 mL | 4.2882 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
The separation and identification of Dopamine 3-O-Sulfate and dopamine 4-O-sulfate in urine of Parkinsonian patients
J Pharmacol Exp Ther 1975 Dec;195(3):441-52.PMID:1195131doi
A method is described for the isolation and measurement of Dopamine 3-O-Sulfate and dopamine 4-O-sulfate. The sulfate isomers of dopamine were synthesized chemically by the reaction of dopamine with sulfuric acid. The isomers were isolated by anion-exchange column chromatography and the identities of the two isomers were confirmed by gas chromatography coupled with mass spectrometry. A method is described for the assay of the two isomers by high-pressure liquid chromatography. When Parkinsonian patients were treated with 4.0 g/day of L-dopa, the amount of Dopamine 3-O-Sulfate excreted in the urine was 19.6 times that of dopamine 4-O-sulfate. When untreated Parkinsonian patients received tracer quantities of 3H-L-dopa either intravenously or orally, 3H-dopamine 3-O-sulfate was the predominant isomer but the amounts were only 3 times those of the 4-isomer. Greater quantities of both isomers were excreted when 3H-L-dopa was given orally as compared to intravenous administration. Since Dopamine 3-O-Sulfate is the predominant sulfate isomer, it is concluded that sulfate conjugation of dopamine could possibly compete with 3-O-methylation in determining the resultant conjugated product.
Direct measurement of dopamine O-sulfate in plasma and cerebrospinal fluid
J Chromatogr 1987 Jan 23;413:17-23.PMID:3558665DOI:10.1016/0378-4347(87)80209-8.
This paper describes a method for measurement of Dopamine 3-O-Sulfate (DA3S) and dopamine 4-O-sulfate (DA4S) in dog and human plasma and dog cerebrospinal fluid. C18 solid-phase extraction columns were utilized for sample preparation. DA3S and DA4S were separated by high-performance liquid chromatography and then quantified by dual-electrode electrochemical detection. [3H]Dopamine O-sulfate (DAS) was used as internal standard. Recovery of authentic DAS added to dog plasma and carried through the entire procedure was 49.9 +/- 6.3% for DA3S (n = 9) and 42.2 +/- 4.3% for DA4S (n = 8). The lower limit of detection (signal-to-noise ratio of 3) was 20 fmol for each DAS isomer. The within-assay coefficient of variation for DA3S in dog plasma averaged 5.8% (range 2.0-12%, n = 5). The between-assay coefficient of variation for DA3S in dog plasma was 3.8% (n = 3). DAS levels in plasma of conscious dogs were 20.3 +/- 6.9 pmol/ml DA3S and 5.91 +/- 3.5 pmol/ml DA4S (n = 5). Cerebrospinal fluid levels were 3.06 +/- 3.22 pmol/ml DA3S and 0.10 +/- 0.18 pmol/ml DA4S in dogs anesthetized with methoxyflurane and nitrous oxide (n = 3). This procedure is also appropriate for use with human plasma; DAS levels were 24.3 +/- 12.8 pmol/ml DA3S and 9.07 +/- 3.9 pmol/ml DA4S (n = 6).
The bicuculline-like properties of dopamine sulfate in rat brain
Life Sci 1984 Sep 3;35(10):1083-90.PMID:6482648DOI:10.1016/0024-3205(84)90073-0.
To determine whether the convulsive action of intraventricularly injected dopamine sulfate, a dopamine metabolite present in rat brain and human cerebrospinal fluid, could be due to its interaction with GABAergic pathway, we compared the convulsive effect of dopamine sulfate with that of bicuculline in the conscious rat and determined the interaction of dopamine sulfate with [3H] GABA binding and uptake in rat brain tissues. The results showed that the convulsive effects of dopamine sulfate and of bicuculline could be abolished by GABA agonists diazepam and muscimol, but not by DA antagonists haloperidol and metoclopramide. In addition they were additive. Both Dopamine 3-O-Sulfate and dopamine-4-O-sulfate, like bicuculline, could displace sodium-independent [3H] GABA binding to rat brain synaptic membranes (IC50 = 400 microM) but had no action on GABA uptake. DA sulfate had no effect on [3H] strychnine binding to rat brain homogenates. This evidence together with the structural resemblance between dopamine sulfate and GABA suggested that the convulsive activity of dopamine sulfate may result from its interaction with central GABA receptors.
Plasma levels and renal clearance of two isomers of dopamine sulfate in patients with essential hypertension
Clin Exp Hypertens A 1988;10(4):561-74.PMID:3390960DOI:10.3109/10641968809033909.
Two isomers of dopamine sulfate, Dopamine 3-O-Sulfate(3-O-S) and dopamine 4-O-sulfate(4-O-S), in the plasma and urine of patients with essential hypertension and normotensive controls were measured by HPLC-fluorometry. The plasma level of 3-O-S was significantly higher in patients with essential hypertension than in normotensive controls (20.9 +/- 2.2 pmol/ml and 16.3 +/- 1.4 pmol/ml respectively, p less than 0.05) and the plasma levels of 4-O-S were slightly increased in hypertensive patients. The plasma level of 3-O-S was correlated with the serum level of creatinine in both normotensives and hypertensives. In hypertensives, the plasma level of 3-O-S was also correlated with that of noradrenaline. However the creatinine clearances were similar in the two groups, the urinary clearance of 3-O-S was slightly lower in patients with essential hypertension than in normotensive controls. These data indicate that the plasma level of 3-O-S is elevated in essential hypertension because of the abnormality in dopaminergic metabolism and renal disturbance in its excretion.
Sample preparation procedure for determination of dopamine sulfate isomers in human urine by high-performance liquid chromatography with dual-electrode electrochemical detection
J Chromatogr 1986 Sep 5;381(2):241-8.PMID:3760083DOI:10.1016/s0378-4347(00)83590-2.
We developed a procedure utilizing small columns of solid-phase extraction material for sample preparation for the determination of dopamine sulfate (DAS) isomers in human urine. Processed sample is then subjected to high-performance liquid chromatography (HPLC) with dual-series-electrode electrochemical detection. Dopamine 3-O-Sulfate (DA-3-S) and dopamine 4-O-sulfate (DA-4-S) were determined using two different HPLC systems. The ratio of the urinary excretion rate of DA-3-S to DA-4-S was relatively constant, but the 24-h excretion rates of total DAS varied widely among individuals. This method should prove useful in future studies concerning the metabolic and physiologic roles of DAS isomers.