Doxycycline
(Synonyms: 多西环素) 目录号 : GC63621
Doxycycline(多西环素DOX)是一种四环素衍生物的广谱抗生素,通过干扰细菌核糖体30S亚基A位点上活化的氨基酰基tRNA的结合来抑制蛋白质合成。
Cas No.:564-25-0
Sample solution is provided at 25 µL, 10mM.
Doxycycline is a tetracycline-derivative wide-spectrum antibiotic, inhibit protein synthesis by interfering with the binding of activated aminoacyl-tRNAs on the A-site of the 30S subunit of bacterial ribosomes. It is used to treat a variety of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor. Doxycycline exhibits anti-tumour and pro-apoptotic activity [1-4].
Doxycycline(0-250 µM; 72h) inhibits cell viability of breast cancer cells and mammospheres[5]. Doxycycline (100 ng/mL, 1 µg/mL; 24 h) reduces proliferation of human cell lines[6].
Doxycycline(20mg /kg; p.o.;2times) treated following mice TBI improved behavioral and motor function suggesting doxycycline's role in preserving murine BBB integrity[7]. Doxycycline(DOX in drinking water (6 mg/ml, 5 ml/day/mice) ; 36 days) significantly minimized the dystrophic phenotype of skeletal and cardiac muscles and improved forelimb muscle strength[8]. The FM(fecal microbiota) of female C57BL/6NCrl mice is significantly altered by administration of doxycycline(doxycycline in the drinking water at 2 mg/ml during weeks 1, 2, and 4) at a concentration of 2 mg/ml in the drinking water[9].
References:
[1]. Cunha BA, Sibley CM, et,al. Doxycycline. Ther Drug Monit. 1982;4(2):115-35. doi: 10.1097/00007691-198206000-00001. PMID: 7048645.
[2].Manchado E, Weissmueller S, et,al. A combinatorial strategy for treating KRAS-mutant lung cancer. Nature. 2016 Jun 30;534(7609):647-51. doi: 10.1038/nature18600. Epub 2016 Jun 22. PMID: 27338794; PMCID: PMC4939262.
[3].Luger AL, Sauer B, et,al. Doxycycline Impairs Mitochondrial Function and Protects Human Glioma Cells from Hypoxia-Induced Cell Death: Implications of Using Tet-Inducible Systems. Int J Mol Sci. 2018 May 17;19(5):1504. doi: 10.3390/ijms19051504. PMID: 29772845; PMCID: PMC5983704.
[4]. Duivenvoorden WC, Popović SV, et,al. Doxycycline decreases tumor burden in a bone metastasis model of human breast cancer. Cancer Res. 2002 Mar 15;62(6):1588-91. PMID: 11912125.
[5]. Zhang L, Xu L, et,al. Doxycycline inhibits the cancer stem cell phenotype and epithelial-to-mesenchymal transition in breast cancer. Cell Cycle. 2017 Apr 18;16(8):737-745. doi: 10.1080/15384101.2016.1241929. Epub 2016 Oct 18. PMID: 27753527; PMCID: PMC5405729.
[6]. Ahler E, Sullivan WJ, et,al. Doxycycline alters metabolism and proliferation of human cell lines. PLoS One. 2013 May 31;8(5):e64561. doi: 10.1371/journal.pone.0064561. PMID: 23741339; PMCID: PMC3669316.
[7]. Malek AJ, Robinson BD, et,al. Doxycycline improves traumatic brain injury outcomes in a murine survival model. J Trauma Acute Care Surg. 2020 Sep;89(3):435-440. doi: 10.1097/TA.0000000000002801. PMID: 32467458.
[8]. Pereira JA, Taniguti AP, et,al. Doxycycline ameliorates the dystrophic phenotype of skeletal and cardiac muscles in mdx mice. Muscle Nerve. 2012 Sep;46(3):400-6. doi: 10.1002/mus.23331. PMID: 22907231.
[9]. Boynton FDD, Ericsson AC, et,al. Doxycycline induces dysbiosis in female C57BL/6NCrl mice. BMC Res Notes. 2017 Nov 29;10(1):644. doi: 10.1186/s13104-017-2960-7. PMID: 29187243; PMCID: PMC5708113.
Doxycycline(多西环素DOX)是一种四环素衍生物的广谱抗生素,通过干扰细菌核糖体30S亚基A位点上活化的氨基酰基tRNA的结合来抑制蛋白质合成。它被用来治疗由细菌和原生动物引起的各种感染。Doxycycline也是一种非特异性基质金属蛋白酶(MMP)抑制剂。Doxycycline具有抗肿瘤和促细胞凋亡活性[1-4]。
Doxycycline (0 - 250µM;72h)抑制乳腺癌细胞和乳腺球细胞活力[5]。Doxycycline (100 ng/mL, 1µg/mL;24 h)降低人细胞系的增殖[6]。Doxycycline (100 ng/mL, 1 µg/mL; 24 h)减少人类细胞系的增殖[7]。
Doxycycline (20mg /kg; p.o.;2times) 在小鼠脑外伤后改善了行为和运动功能,Doxycycline在保护小鼠血脑屏障完整性方面有效[8]。Doxycycline (DOX in drinking water (6 mg/ml, 5 ml/day/mice) ; 36 days)显著减少了骨骼肌和心肌的营养不良表型,并改善了前肢肌肉力量[9]。雌性C57BL/6NCrl小鼠的FM(粪便微生物群)在给予Doxycycline (doxycycline in the drinking water at 2 mg/ml during weeks 1, 2, and 4)后显著改变[10]。
| Cell experiment [1]: | |
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Cell lines |
MCF-7 and MDA-MB-468 cells |
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Preparation Method |
Cells were seeded in 96-well plates at a density of 5000 cells/well. The cells were incubated with doxycycline at concentrations of 0-250 µM for 72 hours. |
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Reaction Conditions |
0-250 µM; 72h |
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Applications |
Doxycycline inhibits cell viability of breast cancer cells and mammospheres. |
| Animal experiment [2]: | |
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Animal models |
C57BL/6J mice |
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Preparation Method |
Mice were assigned to a sham, Traumatic brain injury (TBI), or TBI with doxycycline arm. A moderate TBI was induced utilizing a controlled cortical impactor. The TBI with doxycycline cohort received a dose of doxycycline (20 mg/kg) 2 hours after injury and every 12 hours until postoperative day (POD) 6. |
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Dosage form |
20 mg/kg; p.o ; 2 times |
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Applications |
Doxycycline treated following mice TBI improved behavioral and motor function. |
|
References: [1]. Zhang L, Xu L, et,al. Doxycycline inhibits the cancer stem cell phenotype and epithelial-to-mesenchymal transition in breast cancer. Cell Cycle. 2017 Apr 18;16(8):737-745. doi: 10.1080/15384101.2016.1241929. Epub 2016 Oct 18. PMID: 27753527; PMCID: PMC5405729. |
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| Cas No. | 564-25-0 | SDF | |
| 别名 | 多西环素 | ||
| 分子式 | C22H24N2O8 | 分子量 | 444.43 |
| 溶解度 | DMSO : 125 mg/mL (281.26 mM; Need ultrasonic) | 储存条件 | Store at 4°C, protect from light, stored under argon |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.2501 mL | 11.2504 mL | 22.5007 mL |
| 5 mM | 0.45 mL | 2.2501 mL | 4.5001 mL |
| 10 mM | 0.225 mL | 1.125 mL | 2.2501 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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