DsbA
目录号 : GP21571Disulfide Oxidoreductase Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | DsbA; Thiol:disulfide interchange protein dsbA. | ||
| Amino Acid Sequence | The sequence of the first five N-terminal amino acidswas determined and was found to be Met-Ly-Lys-Ala-Trp. | ||
| Solubility | It is recommended to reconstitute the lyophilized DsbA in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized DsbA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution DsbA should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Formulation | The protein was lyophilized after from a sterile solution containing 50mM sodium phosphate buffer and 100mM sodium chloride. | ||
DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli.
Lyophilized DsbA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution DsbA should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.