Ecdysterone
(Synonyms: β-蜕皮甾酮; 20-Hydroxyecdysone) 目录号 : GN10564
Ecdysterone是一种天然存在的蜕皮激素，能够控制节肢动物的蜕皮（换羽）和变态。
Cas No.:5289-74-7
Sample solution is provided at 25 µL, 10mM.
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Ecdysterone is a naturally occurring ecdysone that controls molting (feathering) and metamorphosis in arthropods[1]. Ecdysterone can promote protein synthesis in skeletal muscle cells[2]. Ecdysterone can be used as a sports nutrition supplement and in metabolic disease research[3, 4].
In vitro, Ecdysterone (0-500μM) treatment of MCF7, MDA-MB-231, and MDA-MB-468 cells for 96h inhibited colony formation, cell proliferation, and induced cell death in the three cell lines[5]. Ecdysterone (1, 50, 100μM) pretreatment of PC12 cells for 24h reversed the cytotoxic effects induced by β-amyloid peptide fragment 25-35 (Aβ25-35) in a dose-dependent manner[6].
In vivo, subcutaneous injection of Ecdysterone (0.6, 0.8, 1mg/kg) in osteoarthritis (OA) rats for 4 weeks significantly improved the pathological state of cartilage tissue, inhibited inflammation in cartilage tissue, and upregulated the expression levels of autophagy markers LC3II/LC3I in cartilage tissue[7].
References:
[1] Lafont R, Balducci C, Dinan L. Ecdysteroids[J]. Encyclopedia, 2021, 1(4): 1267-1302.
[2] Gorelick-Feldman J, MacLean D, Ilic N, et al. Phytoecdysteroids increase protein synthesis in skeletal muscle cells[J]. Journal of agricultural and food chemistry, 2008, 56(10): 3532-3537.
[3] Isenmann E, Ambrosio G, Joseph J F, et al. Ecdysteroids as non-conventional anabolic agent: performance enhancement by ecdysterone supplementation in humans[J]. Archives of toxicology, 2019, 93(7): 1807-1816.
[4] Todorova V, Ivanova S, Chakarov D, et al. Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition[J]. Nutrients, 2024, 16(9): 1382.
[5] Shuvalov O, Fedorova O, Tananykina E, et al. An arthropod hormone, ecdysterone, inhibits the growth of breast cancer cells via different mechanisms[J]. Frontiers in Pharmacology, 2020, 11: 561537.
[6] Su-fen Y, Zhong-jun W, Zheng-qin Y, et al. Protective effect of ecdysterone on PC12 cells cytotoxicity induced by Beta-amyloid 25–35[J]. Chinese journal of integrative medicine, 2005, 11: 293-296.
[7] Tang Y, Mo Y, Xin D, et al. β-ecdysterone alleviates osteoarthritis by activating autophagy in chondrocytes through regulating PI3K/AKT/mTOR signal pathway[J]. American Journal of Translational Research, 2020, 12(11): 7174.
Ecdysterone是一种天然存在的蜕皮激素，能够控制节肢动物的蜕皮（换羽）和变态[1]。Ecdysterone能够促进骨骼肌细胞中的蛋白质合成[2]。Ecdysterone能够用于运动营养补充剂和代谢性疾病研究[3, 4]。
在体外，Ecdysterone（0-500μM）处理MCF7、MDA-MB-231、MDA-MB-468细胞96h，抑制了三种细胞系的集落形成，抑制了细胞增殖并诱导死亡[5]。Ecdysterone（1, 50, 100μM）预处理PC12细胞24h，以剂量依赖性方式逆转了β-淀粉样蛋白肽片段25-35（Aβ25-35）诱导的细胞毒性作用[6]。
在体内，Ecdysterone（0.6, 0.8, 1mg/kg）通过皮下注射治疗骨关节炎（OA）大鼠4周，显著改善了软骨组织的病理状态，抑制了软骨组织中的炎症，上调了软骨组织中自噬标志物LC3II/LC3I的表达水平[7]。
| Cell experiment [1]: | |
Cell lines | MCF7, MDA-MB-231, MDA-MB-468 cells |
Preparation Method | 1000 cells were planted per well on a 6-well plate, in triplicates. 24h later, the cells were treated with 0, 100, 150, 250, 350 or 500μM Ecdysterone for 96h. After treatment, fresh media was added, and cells were grown for 10 days. After the indicated time, cells were fixed with acetic acid/methanol (1:7, v/v) and stained with 0.5% crystal violet. |
Reaction Conditions | 0, 100, 150, 250, 350, 500μM; 96h |
Applications | Photographs of plates with colonies clearly showed that not only the number of colonies, but also their size, was significantly reduced upon treatment with Ecdysterone. |
| Animal experiment [2]: | |
Animal models | Osteoarthritis (OA) rats |
Preparation Method | The OA rats were treated with 30mg/kg 3-methyladenine, 1mg/kg rapamycin, 0.6mg/kg Ecdysterone, 0.8mg/kg Ecdysterone and 1mg/kg Ecdysterone, respectively, with model rats without dosing as control. The administration route for 3-methyladenine and rapamycin was intraperitoneal injection and that for Ecdysterone was subcutaneous injection. The dosing frequency for all the 5 groups was twice a week for 4 weeks. |
Dosage form | 0.6, 0.8, 1mg/kg; twice a week for 4 weeks; s.c. |
Applications | OA was alleviated by Ecdysterone on rats. The expression ratio of LC3II/LC3I was promoted greatly by Ecdysterone. |
References: | |
| Cas No. | 5289-74-7 | SDF | |
| 别名 | β-蜕皮甾酮; 20-Hydroxyecdysone | ||
| 化学名 | (2S,3R,5R,9R,10R,13R,14S,17S)-2,3,14-trihydroxy-10,13-dimethyl-17-[(2R,3R)-2,3,6-trihydroxy-6-methylheptan-2-yl]-2,3,4,5,9,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-6-one | ||
| Canonical SMILES | CC12CCC3C(=CC(=O)C4C3(CC(C(C4)O)O)C)C1(CCC2C(C)(C(CCC(C)(C)O)O)O)O | ||
| 分子式 | C27H44O7 | 分子量 | 486.64 |
| 溶解度 | ≥ 23.25mg/mL in DMSO | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0549 mL | 10.2745 mL | 20.5491 mL |
| 5 mM | 0.411 mL | 2.0549 mL | 4.1098 mL |
| 10 mM | 0.2055 mL | 1.0275 mL | 2.0549 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.50%
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