EGF (1-51), Human
目录号 : GP26015Epidermal Growth Factor (1-51 a
Sample solution is provided at 25 µL, 10mM.
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Epidermal Growth Factor (1-51 a.a.) Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 6.0kDa. The EGF is purified by proprietary chromatographic techniques.
| Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Saccharomyces cerevisiae |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | atRoomtemp. |
| Synonyms | Urogastrone, URG, EGF. | ||
| Amino Acid Sequence | NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW E. | ||
| Solubility | It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized EGF although stable at room temperature for 3 weeks, should be stored desiccated below-18°C.Upon reconstitution Epidermal Growth Factor should be stored at4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 is determined by a cell proliferation assay using murine Balb/c 3T3 cells and is < than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. | ||
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. | ||
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of several epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
The ED50 is determined by a cell proliferation assay using murine Balb/c 3T3 cells and is < than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
Lyophilized EGF although stable at room temperature for 3 weeks, should be stored desiccated below-18°C.Upon reconstitution Epidermal Growth Factor should be stored at4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.