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EGF Receptor Substrate 2 Phospho-Tyr5 Sale

(Synonyms: 表皮生长因子受体底物2 (phospho-tyr5)) 目录号 : GC34253

EGFReceptorSubstrate2(Phospho-Tyr5)是一种活性肽,它来源于表皮生长因子受体自身磷酸化位点(酪氨酸992)。

EGF Receptor Substrate 2 Phospho-Tyr5 Chemical Structure

Cas No.:149261-42-7

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1mg
¥803.00
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5mg
¥2,321.00
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10mg
¥4,106.00
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Sample solution is provided at 25 µL, 10mM.

Description

EGF Receptor Substrate 2 (Phospho-Tyr5) is a biologically active peptide derived from an autophosphorylation site (Tyr992) of epidermal growth factor receptor (EGFR).

DADEpYLIPQQG is a generalized PTPase substrate. Both CAV GST-VP2 and TLMV GST-ORF2 are shown to have PTPase activity using both ENDpYINASL and DADEpYLIPQQG. Steady state activities for the reaction of 5 μg of CAV GST-VP2 with ENDpYINASL and DADEpYLIPQQG are 100 and 208%, respectively, of those seen for 2 units of TC-PTP[1]. In the context of DADEpYLIPQQG, the minimal sizes recognized by PTPα are either ADEpYLI or DADEpY-NH2. The kcat/Km value for the parent peptide DADEpYLIPQQG (EGFR988-998) is 1090-fold higher than Tyr(P)[2].

[1]. Peters MA, et al. Chicken anemia virus VP2 is a novel dual specificity protein phosphatase. J Biol Chem. 2002 Oct 18;277(42):39566-73. Epub 2002 Jul 31. [2]. Wu L, et al. Comparative kinetic analysis and substrate specificity of the tandem catalytic domains of the receptor-like protein-tyrosine phosphatase alpha. J Biol Chem. 1997 Mar 14;272(11):6994-7002.

实验参考方法

Kinase experiment:

Briefly, the assays are performed in 50 μL volumes in a microtiter plate using the generalized PTPase substrates ENDpYINASL and DADEpYLIPQQG (where pY represents phosphotyrosine) and assay buffer containing 25 mM Tris-HCl (pH 7.4), 50 mM NaCl, 2 mM EDTA, 5 mM dithiothreitol, 0.01% Brij 35, and 1 mg of bovine serum albumin/mL. The reactions are started by the addition of 5 μg of either CAV GST-VP2, GST, CAV GST-VP2 containing the C95S mutation, or TLMV GST-ORF2 or 2 units of the positive control T cell protein-tyrosine phosphatase (TC-PTP) in assay buffer. Control reactions are assayed with either ENDpYINASL substrate alone, DADEpYLIPQQG substrate alone, CAV GST-VP2 without substrate, CAV GST-VP2 containing the C95S mutation without substrate, TLMV GST-ORF2 without substrate, TC-PTP without substrate, or assay buffer with neither enzyme nor substrate. A phosphate standard curve is derived using a supplied phosphate standard. The reactions are terminated by the addition of the malachite green detection reagent.

References:

[1]. Peters MA, et al. Chicken anemia virus VP2 is a novel dual specificity protein phosphatase. J Biol Chem. 2002 Oct 18;277(42):39566-73. Epub 2002 Jul 31.
[2]. Wu L, et al. Comparative kinetic analysis and substrate specificity of the tandem catalytic domains of the receptor-like protein-tyrosine phosphatase alpha. J Biol Chem. 1997 Mar 14;272(11):6994-7002.

化学性质

Cas No. 149261-42-7 SDF
别名 表皮生长因子受体底物2 (phospho-tyr5)
Canonical SMILES H-Asp-Ala-Asp-Glu-{pTyr}-Leu-Ile-Pro-Gln-Gln-Gly-OH
分子式 C54H82N13O24 分子量 1328.28
溶解度 H2O : 50 mg/mL (37.64 mM; Need ultrasonic) 储存条件 Store at -20°C
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1 mg 5 mg 10 mg
1 mM 0.7529 mL 3.7643 mL 7.5285 mL
5 mM 0.1506 mL 0.7529 mL 1.5057 mL
10 mM 0.0753 mL 0.3764 mL 0.7529 mL
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