Elastase from porcine pancreas
(Synonyms: 弹性蛋白酶) 目录号 : GC61737
猪胰弹性蛋白酶Porcine pancreatic elastase (PPE)是最显著的丝氨酸蛋白酶之一,可以诱导蛋白酶抗蛋白酶失衡,并应用于肺气肿和慢性阻塞性肺疾病(COPD)的动物模型中。
Cas No.:39445-21-1
Sample solution is provided at 25 µL, 10mM.
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Biological Activity: 30 U/mg solid
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| Cell experiment [1]: | |
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Cell lines |
Mouse LE cells (mouse alveolar type II epithelial cells) |
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Preparation Method |
Cells were treated with 0-60 mU/ml Elastase from porcine pancreas for 24 h |
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Reaction Conditions |
0-60 mU/ml Elastase from porcine pancreas ;24 h |
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Applications |
Elastase from porcine pancreas treatment increased placenta growth factor (PlGF) expression and secretion in LE cells. |
| Animal experiment [2]: | |
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Animal models |
C57BL/6 mice |
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Preparation Method |
Mice were intratracheally instillated with saline (CON) or 100 mU/ml Elastase from porcine pancreas weekly for one month. |
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Dosage form |
100 mU/ml Elastase from porcine pancreas; weekly for one month;i.p |
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Applications |
Elastase from porcine pancreas instillation increase PlGF expression, activation of JNK and p38 MAPK, and PlGF secretion in the pulmonary cells of mice. |
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References: [1]. Hou HH, Cheng SL, et, al. Elastase induced lung epithelial cell apoptosis and emphysema through placenta growth factor. Cell Death Dis. 2013 Sep 5;4(9):e793. doi: 10.1038/cddis.2013.329. PMID: 24008737; PMCID: PMC3789187. |
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Elastase from porcine pancreas (PPE), as one of the most prominent serine proteases,it can induce protease antiprotease imbalance and has been applied in an animal model of emphysema and Chronic pulmonary obstructive disease (COPD).It has 240 amino acids and a molecular weight of 26 kD. The conserved active site of elastase consists of three residues of serine 214, histidine 71, and aspartate 119 [1-3].
Elastase from porcine pancreas (0-60 mU/ml;24 h) treatment increased placenta growth factor (PlGF) expression and secretion in LE cells[4].
Elastase from porcine pancreas (100 mU/ml; weekly for one month;i.p) instillation increase PlGF expression, activation of JNK and p38 MAPK, and PlGF secretion in the pulmonary cells of mice[4]. Elastase from porcine pancreas can induces emphysema in hamsters[5].
References:
[1]. Shapiro SD. Animal models for COPD. Chest 2000; 117: 223S-227S.
[2]. M. WÜrtele, M. Hahn, K. Hilpert, W. HÖhne. Acta Crystallogr. D Biol. Crystallogr., 56 (2000), p. 520
[3]. Shotton DM, et, al. Amino-acid sequence of porcine pancreatic elastase and its homologies with other serine proteinases. Nature. 1970 Feb 28;225(5235):802-6.
[4]. Hou HH, Cheng SL, et, al. Elastase induced lung epithelial cell apoptosis and emphysema through placenta growth factor. Cell Death Dis. 2013 Sep 5;4(9):e793. doi: 10.1038/cddis.2013.329. PMID: 24008737; PMCID: PMC3789187.
[5]. Stone PJ, Lucey EC, et, al. Lnduction and exacerbation of emphysema in hamsters with human neutrophil elastase inactivated reversibly by a peptide boronic acid. Am Rev Respir Dis. 1990 Jan;141(1):47-52. doi: 10.1164/ajrccm/141.1.47. PMID: 2297186.
猪胰弹性蛋白酶Porcine pancreatic elastase (PPE)是最显著的丝氨酸蛋白酶之一,可以诱导蛋白酶抗蛋白酶失衡,并应用于肺气肿和慢性阻塞性肺疾病(COPD)的动物模型中。它有240个氨基酸和分子量为26 kD。弹性蛋白酶的保守活性位点由三个谷氨酸214、组氨酸71和天冬氨酸119残基组成[1-3]。
Elastase from porcine pancreas (0-60 mU/ml PPE;24 h)处理可以增加LE细胞中胎盘生长因子(PlGF)的表达和分泌[4]。
Elastase from porcine pancreas (100 mU/ml PPE; weekly for one month;i.p)可以增加小鼠肺细胞中PlGF的表达、JNK和p38 MAPK的活化以及PlGF的分泌[4]。Elastase from porcine pancreas可以在诱导仓鼠肺气肿[5]。
| Cas No. | 39445-21-1 | SDF | |
| 别名 | 弹性蛋白酶 | ||
| 分子式 | C1135H1759N331O346S10 | 分子量 | 25898.13 |
| 溶解度 | H2O : 50 mg/mL (ultrasonic and adjust pH to 9 with NaOH); H2O : 1 mg/mL (Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.0386 mL | 0.1931 mL | 0.3861 mL |
| 5 mM | 0.0077 mL | 0.0386 mL | 0.0772 mL |
| 10 mM | 0.0039 mL | 0.0193 mL | 0.0386 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Anti-elastase and anti-hyaluronidase activity of phosvitin isolated from hen egg yolk
Br Poult Sci 2020 Feb;61(1):17-21.PMID:31661977DOI:10.1080/00071668.2019.1686124.
1. Phosvitin, a major phosphoprotein found in egg yolk, has strong antioxidant activity. Activation of elastase, collagenase, and hyaluronidase by reactive oxygen species are related to the degradation of ECM and skin aging. The objective of this study was to determine the anti-elastase and anti-hyaluronidase activity of phosvitin.2. Elastase from porcine pancreas and hyaluronidase from bovine testes were used to study the inhibitory activity of phosvitin. To elucidate the mechanism of enzyme inhibition, a Lineweaver-Burk plot was constructed.3. Phosvitin inhibited elastase and hyaluronidase activity in a dose-dependent manner. The IC50 value of phosvitin was 31.6 μg/ml and 1,270 μg/ml against elastase and hyaluronidase, respectively. The analysis of elastase and hyaluronidase kinetics indicated that the apparent Michaelis constant (appKm) was increased by phosvitin but the Vmax value was not affected.4. In conclusion, phosvitin exhibited competitive inhibitory activity against elastase and hyaluronidase. Thus, phosvitin could be used as a natural anti-aging agent in the cosmetics industry.
Reverse catalysis of Elastase from porcine pancreas in frozen aqueous systems
Biol Chem 1998 Jan;379(1):71-4.PMID:9504720doi
The reverse action of a trypsin-free elastase isolated from porcine pancreas was studied in frozen aqueous systems. Under frozen state conditions, porcine pancreatic elastase was able to catalyse peptide bond formation more effectively than in solution at room temperature. The acceptance of free amino acids as nucleophilic amino components indicates a changed specificity of the endoprotease in frozen reaction mixtures. In elastase-catalysed formation of Ser-, Ile- and Val-X-bonds in frozen aqueous reaction mixtures, peptide yields obtained depended on the P1 amino acid and the acyl donor chain length.