Endothelin-1 (1-31) (Human) TFA
目录号 : GC69064Endothelin-1 (1-31) (Human) TFA 是一种有效的血管收缩剂和血压升高剂。Endothelin-1 (1-31) (Human) TFA 来源于 chymase 对大 ET-1 的选择性水解。
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Endothelin-1 (1-31) (Human) TFA is a potent vasoconstrictor and pertensive agent. Endothelin-1 (1-31) (Human) TFA is derived from the selective drolysis of big ET-1 by cmase[1].
Endothelin-1 (1-31) (Human) (100 pM-100 nM; 24 h) TFA 诱导人系膜细胞增殖[2]。
Endothelin-1 (1-31) (Human) (100 nM; 0-10 min) TFA 在人系膜细胞中诱导 ERK 激活[2]。
Cell Proliferation Assay[2]
| Cell Line: | Human mesangial cells |
| Concentration: | 100 pM-100 nM |
| Incubation Time: | 24 h |
| Result: | Caused an increase in [3H]-tmidine incorporation into the cells in a concentration-dependent manner. |
Western Blot Analysis[2]
| Cell Line: | Human mesangial cells |
| Concentration: | 100 nM |
| Incubation Time: | 0, 5, 10, 15 and 30 min |
| Result: | ERK activities rapidly increased 2.45-fold at 5 min and peaked at 10 min. The activities of both ERKs rapidly declined, returning to the baseline control value 30 min after stimulation. |
ET-1 (1-31) (100 nM; single dose) TFA 诱导小鼠肠系膜动脉收缩。收缩可能是由 ETA 受体介导的，并可能随着年龄的增长而增加。在目前的慢性糖尿病的情况下，男性和女性之间存在明显差异[1]。
| Animal Model: | ICR mice, Streptozocin -induced diabetic model[1] |
| Dosage: | 100 nM |
| Administration: | In the organ bath, single dose |
| Result: | In the 1-week control (but not diabetic) group, induced contraction and the contractile response was significantly greater in female mice than in male mice, and there was no significant difference in either male or female mice between the age-matched controls and the diabetic mice. In the 8-weeks group, the contraction was or tended to be increased compared with the corresponding 1-week group in all mice. Although in male mice this contraction was not different between control and diabetic groups, it was significantly greater in diabetic female mice than in the control female mice and in female diabetic mice than in male diabetic mice. The contraction was inhibited by ETA receptor inhibitor. |
[1]. Matsumoto T, et al. Gender differences in vascular reactivity to endothelin-1 (1-31) in mesenteric arteries from diabetic mice. Peptides. 2008 Aug;29(8):1338-46.
[2]. Yoshizumi M, et al. Effect of endothelin-1 (1-31) on human mesangial cell proliferation. Jpn J Pharmacol. 2000 Oct;84(2):146-55.
| Cas No. | SDF | Download SDF | |
| 分子式 | C164H237F3N38O49S5 | 分子量 | 3742.18 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.2672 mL | 1.3361 mL | 2.6722 mL |
| 5 mM | 0.0534 mL | 0.2672 mL | 0.5344 mL |
| 10 mM | 0.0267 mL | 0.1336 mL | 0.2672 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。