Endothelin 1 (swine, human) (TFA)
目录号 : GC64457
Endothelin 1 (swine, human) (TFA)是一种具有人和猪内皮素1（ET-1）序列的合成肽，是有效的内源性血管收缩剂。
Cas No.:394693-38-0
Sample solution is provided at 25 µL, 10mM.
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Endothelin 1 (swine, human) (TFA) is a synthetic peptide with human and porcine Endothelin 1 (ET-1) sequences and is a potent endogenous vasoconstrictor[1]. Endothelin 1 acts through ETA and ETB receptors and has proinflammatory and profibrotic effects[2]. Endothelin 1 is a potent vasoconstrictor and mitogen that contributes to elevated blood pressure and associated vascular remodeling and target organ damage[3]. Endothelin 1 can act as an autocrine/paracrine growth factor in porcine ovarian granulosa cells and human cancer cell lines[4, 5].
In vitro, treatment of porcine ovarian (granulosa) cell monolayers with Endothelin 1 (swine, human) (TFA) (100nM) rapidly increased intracellular soluble inositol polyphosphate content, elevated Ca2+ concentration, and inhibited follicle-stimulating hormone (FSH)-stimulated progesterone and cAMP accumulation[6].
References:
[1] Al-Jiffry B O, Meedeniya A C B, Chen J W, et al. Endothelin-1 induces contraction of human and Australian possum gallbladder in vitro[J]. Regulatory peptides, 2001, 102(1): 31-39.
[2] Kowalczyk A, Kleniewska P, Kolodziejczyk M, et al. The role of endothelin-1 and endothelin receptor antagonists in inflammatory response and sepsis[J]. Archivum immunologiae et therapiae experimentalis, 2015, 63: 41-52.
[3] Shreenivas S, Oparil S. The role of endothelin-1 in human hypertension[J]. Clinical hemorheology and microcirculation, 2007, 37(1-2): 157-178.
[4] Flores J A, Quyyumi S, Leong D A, et al. Actions of endothelin-1 on swine ovarian (granulosa) cells[J]. Endocrinology, 1992, 131(3): 1350-1358.
[5] Shichiri M, Hirata Y, Nakajima T, et al. Endothelin-1 is an autocrine/paracrine growth factor for human cancer cell lines[J]. The Journal of clinical investigation, 1991, 87(5): 1867-1871.
[6] Kamada S, Kubota T, Hirata Y, et al. Endothelin-1 is an autocrine/paracrine regulator of porcine granulosa cells[J]. Journal of endocrinological investigation, 1993, 16: 425-431.
Endothelin 1 (swine, human) (TFA)是一种具有人和猪内皮素1（ET-1）序列的合成肽，是有效的内源性血管收缩剂[1]。Endothelin 1通过ETA和ETB受体发挥作用，还具有促炎症反应和促纤维化作用[2]。Endothelin 1是一种强大的血管收缩剂和有丝分裂原，有助于血压升高以及相关的血管重塑和靶器官损伤[3]。Endothelin 1可以充当猪卵巢颗粒细胞和人类癌细胞系中的自分泌/旁分泌生长因子[4, 5]。
在体外，Endothelin 1（100nM）处理猪卵巢（颗粒）细胞单层培养物，快速增加了细胞内可溶性肌醇多磷酸酯含量，升高了Ca2+浓度，抑制了促卵泡激素（FSH）刺激的孕酮和cAMP积累[6]。
| Cell experiment [1]: | |
Cell lines | Swine ovarian (granulosa) cells |
Preparation Method | Swine ovarian (granulosa) cells monolayer cultures were established in M199 (which contains 0.05mg/liter inositol) supplemented with 3% FBS and porcine insulin (3pg/mL) at an initial plating density of 1×107 viable tells/35-mm tissue culture. After 48h, the incubation medium was exchanged with fresh serum-free M199 supplemented with 0.1% BSA. After 24h, cells were prelabeled with [3H]myoinositol dishes by exposing them to M199 containing 0.1% BSA and 2.5μCi/mL [3H]myoinositol for 18h. Thereafter, cells were washed twice in M199 with 0.1% BSA and incubated for 1h in the presence of 10mM unlabeled inositol. During the last 15min of this “cold chase” period, 30μL of a 0.5M stock LiCl solution were added to each dish ( final concentration, 10mM). At designated intervals, medium alone (control) or Endothelin 1 (final concentration, 100nM) was added in 80μL aliquots. The reaction was stopped by the addition of ice-cold chloro- form-methanol (1:2, vol/vol). Samples were maintained on ice for about 1h while cells were scraped from the culture dishes and centrifuged in bio-vials. [3H]Inositol phosphates were separated by anion exchange chromatography. |
Reaction Conditions | 100nM; 15min |
Applications | Endothelin 1 exposure leads to rapid appearance of soluble inositol polyphosphates in granulosa cells. |
References: | |
| Cas No. | 394693-38-0 | SDF | Download SDF |
| 分子式 | C111H160F3N25O34S5 | 分子量 | 2605.92 |
| 溶解度 | Water : 50 mg/mL (19.19 mM; Need ultrasonic) | 储存条件 | Store at -20°C, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.3837 mL | 1.9187 mL | 3.8374 mL |
| 5 mM | 0.0767 mL | 0.3837 mL | 0.7675 mL |
| 10 mM | 0.0384 mL | 0.1919 mL | 0.3837 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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