(-)-epigallocatechin
(Synonyms: (-)-表没食子儿茶素; Epigallocatechin; L-Epigallocatechin) 目录号 : GC17242A green tea polyphenol
Cas No.:970-74-1
Sample solution is provided at 25 µL, 10mM.
(-)-epigallocatechin is a polyphenol in green tea [1].
(-)-epigallocatechin (EGC) plays an important role in cell growth inhibition, apoptosis and bone metabolism.
(-)-epigallocatechin is a polyphenol in green tea. In MCF-7 and MDA-MB-231 breast cancer cell lines, EGC significantly inhibited cell growth and induced apoptosis, which were independent of p53 and required Fas signaling [1]. In H1299 cells, EGC (10-40 µM) reduced cell viability in a dose dependent way. In Hep-3B cells, EGC (40/80 µM) also reduced cell viability. However, EGC (20 µM) slightly increased cell viabilities in SK-Hep-1 and OECM-1 cells but significantly decreased cell viabilities at concentrations up to 40 µM. EGC exhibited cytotoxicity with IC50 values of 26, 33 and 22 µM in H1299, OECM-1 and SAS cells, respectively. In H1299, OECM-1 and SAS cells, EGC (40 µM) induced apoptosis by 30, 28 and 24%, respectively. In H1299 cells, EGC (40 µM) inhibited hTERT promoter activity in a time- and dose- dependent way and also inhibited hTERT mRNA expression, which was important for cell proliferation [2].
In ICR mice, EGC (0.5 and 1.0 g/kg/d) significantly inhibited platelet aggregation by 18.4% and 25.6% respectively and also significantly prolonged blood clotting time (BCT) and bleeding time (BT), which suggested that the blood anticoagulation and antiplatelet activity of EGC [3].
References:
[1]. Vergote D, Cren-Olivé C, Chopin V, et al. (-)-Epigallocatechin (EGC) of green tea induces apoptosis of human breast cancer cells but not of their normal counterparts. Breast Cancer Res Treat, 2002, 76(3): 195-201.
[2]. Lin SC, Li WC, Shih JW, et al. The tea polyphenols EGCG and EGC repress mRNA expression of human telomerase reverse transcriptase (hTERT) in carcinoma cells. Cancer Lett, 2006, 236(1): 80-88.
[3]. Chen XQ, Wang XB, Guan RF, et al. Blood anticoagulation and antiplatelet activity of green tea (-)-epigallocatechin (EGC) in mice. Food Funct, 2013, 4(10): 1521-1525.
Cas No. | 970-74-1 | SDF | |
别名 | (-)-表没食子儿茶素; Epigallocatechin; L-Epigallocatechin | ||
化学名 | (2R,3R)-2-(3,4,5-trihydroxyphenyl)chroman-3,5,7-triol | ||
Canonical SMILES | O[C@@H]1CC2=C(C=C(O)C=C2O)O[C@@H]1C3=CC(O)=C(O)C(O)=C3 | ||
分子式 | C15H14O7 | 分子量 | 306.27 |
溶解度 | DMF: 25 mg/ml,DMSO: 15 mg/ml,Ethanol: 5 mg/ml,PBS (pH 7.2): 1 mg/ml | 储存条件 | Store at -20°C,protect from light |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.2651 mL | 16.3255 mL | 32.6509 mL |
5 mM | 0.653 mL | 3.2651 mL | 6.5302 mL |
10 mM | 0.3265 mL | 1.6325 mL | 3.2651 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet