EPO a Human
目录号 : GP20193促红细胞生成素-α 人类重组体
Sample solution is provided at 25 µL, 10mM.
Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166 amino acids and having a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 36-40kDa. EPO-a is purified by proprietary chromatographic techniques.
| Purity | >95% as analyzed by SDS-PAGE; > 95% as analyzed by HPLC | Source | Chinese Hamster Ovary Cells(CHO). |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Erythropoietin-Alpha; EPO-a; EPO-alpha; Epoetin; EP; MGC138142. | ||
| Amino Acid Sequence | APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR. | ||
| Solubility | It is recommended to reconstitute the lyophilized Epoetin-a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Upon receiving, this product remains stable for up to 6 months at lower than -70℃, Upon reconstitution, the product should be stablefor up to 1 week at 4℃ or up to 3 months at -20℃. For long term storage it is recommended that a carrier protein (example 0.1% BSA)be added, Avold repeated freeze-thaw cycles. | ||
| Biological Activity | ED50 < 1.0 ng/ml, measured in a cell proliferation assay using TF-1 human erythroleukemic cells, corresponding to a specific activity of > 1.0 × 106units/mg | ||
| Endotoxin Level | < 0.2 EU/ug of protein by gel clotting method | ||
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddHzO or PBS up to 100 ug/ml. | ||
| Formulation | Each mg of lyophilized powder contains 0.59 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid. | ||
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
ED50 < 1.0 ng/ml, measured in a cell proliferation assay using TF-1 human erythroleukemic cells, corresponding to a specific activity of > 1.0 × 106units/mg
Upon receiving, this product remains stable for up to 6 months at lower than -70℃, Upon reconstitution, the product should be stablefor up to 1 week at 4℃ or up to 3 months at -20℃. For long term storage it is recommended that a carrier protein (example 0.1% BSA)be added, Avold repeated freeze-thaw cycles.