Erythrosin B (Erythrosin extra bluish)
(Synonyms: 赤藓红B,Erythrosin extra bluish) 目录号 : GC33466
A red fluorescein dye and an inhibitor of dopamine reuptake
Cas No.:16423-68-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment: | The alkaline single-cell gel electrophoresis assay (comet assay) is performed. Briefly, 2×105 HepG2 cells are seeded in a 24-well plate for 24 h. The cells are then treated with Erythrosine B at 0.1, 0.2, 2.0, 10.0, 25.0, 50.0 or 70.0 μg/mL (final concentration) for 4 h; vehicle control (0.7% DMSO) and positive control (doxorubicin0.3 μg/mL) treatments are also performed. The HepG2 cell suspension is mixed with 37°C low-melting point agarose and transferred to normal-melting point agarose-coated slides. One hundred randomly chosen nucleoids are analyzed per treatment, and a total of three independent experiments are performed[1]. |
References: [1]. Chen W, et al. Establishing an experimental rat model of photodynamically-induced retinal vein occlusion using erythrosin B. Int J Ophthalmol. 2014 Apr 18;7(2):232-8. | |
Erythrosin B is a red fluorescein dye and an inhibitor of dopamine reuptake (IC50 = 45 ?M in rat brain synaptosomes).1,2,3 It has been used in the colorimetric and fluorescent determination of protein concentrations in vitro.1,2 Formulations containing erythrosine B have been used as color additives in food and pharmaceutical preparations.
1.Ma, C.Q., Li, K.A., and Tong, S.Y.Determination of proteins by fluorescence quenching of erythrosin BAnal. Chim. Acta333(1-2)83-88(1996) 2.Soedjak, H.S.Colorimetric micromethod for protein determination with erythrosin BAnal. Biochem.220(1)142-148(1994) 3.Lafferman, J.A., and Silbergeld, E.K.Erythrosin B inhibits dopamine transport in rat caudate synaptosomesScience205(4404)410-412(1979)
| Cas No. | 16423-68-0 | SDF | |
| 别名 | 赤藓红B,Erythrosin extra bluish | ||
| Canonical SMILES | O=C1OC2(C3=C(OC4=C2C=C(I)C([O-])=C4I)C(I)=C([O-])C(I)=C3)C5=C1C=CC=C5.[Na+].[Na+] | ||
| 分子式 | C20H6I4Na2O5 | 分子量 | 879.86 |
| 溶解度 | Water : 20.7 mg/mL (23.53 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.1365 mL | 5.6827 mL | 11.3654 mL |
| 5 mM | 0.2273 mL | 1.1365 mL | 2.2731 mL |
| 10 mM | 0.1137 mL | 0.5683 mL | 1.1365 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。