Ethambutol HCl
(Synonyms: 盐酸乙胺丁醇; Emb dihydrochloride) 目录号 : GC15651An antimycobacterial compound
Cas No.:1070-11-7
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Nocodazole is a tubulin production inhibitor, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 value of 0.21 μM, 0.53 μM and 0.64 μM, respectively [1].
Nocodazole is an inhibitor of microtubule polymerization which inhibits insulin-stimulated glucose transport. It impairs the morphology and directionality of migrating medial gan-glionic eminence cells and disrupts microtubules by binding to β-tubulin. Nocodazole also prevents the formation of one of the two interchain disulfide linkages and impairs the transport of vesicles.
As a high-affinity ligand for the cancer-related kinases such as Abl phosphorylated, c-Kit, BRAF, and MEK, nocodazole inhibits BRAF, Abl phosphorylated, c-Kit and MEK with Kd value of 1.8 μM, 0.091 μM, 1.6 μM, and 1.6 μM, respectively. Additionally, nocodazole inhibits phosphorylated Abl(E255K), phosphorylated Abl(T315I), BRAF(V600E) and PI3Kγ with the Kd value of 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Mitotic cells which incubated with different concentrations of paclitaxel, released from the nocodazole block, are inhibited from progressing to G1 phase in 6 hours with a median inhibitory concentration of 4 nM. In the absence of nocodazole, the cells which pretreated with nocodazole exposed to paclitaxel only form free-floating microtubules, while pretreated cells exposed to paclitaxel organized microtubules [1,2].
In vivo, the therapeutic efficacy of a treatment of combination of nocodazole (5 mg/kg/three times per week) and ketoconazole (50 mg/kg/three times per week) was tested by treating athymic mice bearing COLO 205 tumor xenografts. The antitumor effects of nocodazole were significantly potentiated by ketoconazole in mice after 6 wk of treatment. The tumor volume and tumor weight of the mice treated with a combination of ketoconazole and nocodazole are significantly reduced as compared with those treated with ketoconazole or nocodazole alone. Treatment with the combination of ketoconazole and nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with nocodazole or ketoconazole alone [3].
References:
[1]. Park H, Hong S, Hong S. Nocodazole is a High-Affinity Ligand for the Cancer-Related Kinases ABL, c-KIT, BRAF, and MEK. ChemMedChem, 2012, 7(1): 53-56.
[2]. Long bh,fairchild cr. Paclitaxel inhibits progression of mitotic cells to g(1) phase by interference with spindle formation without affecting other microtubule functions during anaphase and telephase. Cancer Research, 1994, 54(16): 4355-4361.
[3]. Wang YJ, Jeng JH, Chen RJ, et al. Ketoconazole potentiates the antitumor effects of nocodazole: In vivo therapy for human tumor xenografts in nude mice. Molecular Carcinogenesis, 2002, 34(4): 199-210.
Cas No. | 1070-11-7 | SDF | |
别名 | 盐酸乙胺丁醇; Emb dihydrochloride | ||
化学名 | 2-[2-(1-hydroxybutan-2-ylamino)ethylamino]butan-1-ol;dihydrochloride | ||
Canonical SMILES | CCC(CO)NCCNC(CC)CO.Cl.Cl | ||
分子式 | C10H24N2O2.2HCl | 分子量 | 277.23 |
溶解度 | ≥ 12.65mg/mL in DMSO | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.6071 mL | 18.0356 mL | 36.0711 mL |
5 mM | 0.7214 mL | 3.6071 mL | 7.2142 mL |
10 mM | 0.3607 mL | 1.8036 mL | 3.6071 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。