Ethoxyquin
(Synonyms: 乙氧基喹啉) 目录号 : GC32965
An antioxidant used in animal feed
Cas No.:91-53-2
Sample solution is provided at 25 µL, 10mM.
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Ethoxyquin is an antioxidant that is widely used in animal feed to protect against lipid peroxidation and fat rancidity in chicken, salmon, and beef.1 In vitro, ethoxyquin protects human lymphocytes against hydrogen peroxide-induced DNA damage and reduces micronuclei formation.2 In vivo, it reduces the number of chromosome aberrations, micronuclei, and dominant lethal mutations induced by cyclophosphamide in mice, rats, and Chinese hamsters.3,4 Ethoxyquin also induces chromosome aberrations such as atypical translocations, breaks, and dicentrics in human lymphocytes and CHO cells as well as in vivo kidney and bladder damage in rats.1
1.Blaszczyk, A., Augustyniak, A., and Skolimowski, J.Ethoxyquin: An antioxidant used in animal feedInt. J. Food Sci.2013585931(2013) 2.Blaszczyk, A., and Skolimowski, J.Comparative analysis of cytotoxic, genotoxic and antioxidant effects of 2,2,4,7-tetramethyl-1,2,3,4-tetrahydroquinoline and ethoxyquin on human lymphocytesChem. Biol. Interact.162(1)70-80(2006) 3.Renner, H.W.Antimutagenic effect of an antioxidant in mammalsMutat. Res.135(2)125-129(1984) 4.Renner, H.W., and Knoll, M.Antimutagenic effects on male germ cells of miceMutat. Res.140(2-3)127-129(1984)
Kinase experiment: | To evaluate the impact of Ethoxyquin (EQ) on Paclitaxel’s (PTX’s) ability to kill breast cancer cells, conditions for culturing 4 cancer cell lines and measuring the ATP levels are optimized for the 96-well plate format. Briefly, 1,500 cells/well in media are plated in 96-well plates for 24 hours. Constant concentrations of PTX with or without Ethoxyquin are added to the wells for another 24 hours. Cellular ATP levels are measured using kit according tomanufacturer ’s protocol[2]. |
Cell experiment: | Briefly, DRGs are harvested from embryonic day 14.5 rats according to standard protocols, then cells are plated onto collagen-coated glass coverslips and allowed to extend neurites for 24 hours in media (Neurobasal medium, 50 mM pencillin-streptomycin (PS), 0.2% FBS, 0.5 mM glutamine, 1×B-27 supplement, 0.2% glucose,10 ng/mL glial cell line-derived neurotrophic factor). Ethoxyquin (EQ) or vehicle control is added to the wells for another 24-hour incubation. DRG cells are fixed with 4% paraformaldehyde and stained with anti-βIII-tubulin antibody to delineate the axons. Axon lengths are measured in multiple fields using a random sampling method[2]. |
Animal experiment: | To examine the effect of Ethoxyquin (EQ) on Paclitaxel’s (PTX’s) ability to reduce tumor burden in vivo, a mouse model in which breast cancer cell line SUM-159 (3×106 tumor cells suspended in phosphate-buffered saline in a final volume of 0.15 mL) is injected subcutaneously into adult male nude mice. When the tumor size reaches 5 mm in diameter, the animals are randomly assigned to PTX or PTX with Ethoxyquin groups. Ethoxyquin is given by intraperitoneal administration on a daily basis for 3 weeks. At the end of 3 weeks, animals are euthanized and tumor size and weight are measured (n=5 per group)[2]. |
References: [1]. Sadikot T, et al. Development of a high-throughput screening cancer cell-based luciferase refolding assay for identifying Hsp90 inhibitors. Assay Drug Dev Technol. 2013 Oct;11(8):478-88. | |
| Cas No. | 91-53-2 | SDF | |
| 别名 | 乙氧基喹啉 | ||
| Canonical SMILES | CC1=CC(C)(C)NC2=C1C=C(OCC)C=C2 | ||
| 分子式 | C14H19NO | 分子量 | 217.31 |
| 溶解度 | DMSO : ≥ 50 mg/mL (230.09 mM);Water : < 0.1 mg/mL (insoluble) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.6017 mL | 23.0086 mL | 46.0172 mL |
| 5 mM | 0.9203 mL | 4.6017 mL | 9.2034 mL |
| 10 mM | 0.4602 mL | 2.3009 mL | 4.6017 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet