Ethyl gallate
(Synonyms: 没食子酸乙酯) 目录号 : GC31421
An ellagitannin with diverse biological activities
Cas No.:831-61-8
Sample solution is provided at 25 µL, 10mM.
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Ethyl gallate is an ellagitannin that has been found in A. nilotica and has diverse biological activities.1,2,3,4,5 It inhibits squalene epoxidase with an IC50 value of 4.2 ?M for the rat enzyme.1 Ethyl gallate scavenges DPPH radicals in a cell-free assay (IC50 = 4.96 ?g/ml).2 It inhibits the proliferation of, and induces apoptosis in, HL-60 leukemia cells when used at a concentration of 100 ?M.3 Ethyl gallate is also active against the Gram-negative bacterium M. catarrhalis (IC50 = 1.15 ?g/ml).4 It reverses decreases in mean arterial blood pressure (MAP) and systemic vascular resistance in a dog model of E. coli-induced septic shock when administered at a dose of 80 mg/kg.5
1.Abe, I., Kashiwagi, Y., Noguchi, H., et al.Ellagitannins and hexahydroxydiphenoyl esters as inhibitors of vertebrate squalene epoxidaseJ. Nat. Prod.64(8)1010-1014(2001) 2.Kalaivani, T., Rajasekaran, C., and Mathew, L.Free radical scavenging, cytotoxic, and hemolytic activities of an active antioxidant compound ethyl gallate from leaves of Acacia nilotica (L.) Wild. Ex. Delile subsp. indica (Benth.) BrenanJ. Food Sci.76(6)T144-T149(2011) 3.Kim, W.-H., Song, H.-O., Choi, H.-J., et al.Ethyl gallate induces apoptosis of HL-60 cells by promoting the expression of caspases-8, -9, -3, apoptosis-inducing factor and endonuclease GInt. J. Mol. Sci.13(9)11912-11922(2012) 4.Cueva, C., Mingo, S., Mu?oz-González, I., et al.Antibacterial activity of wine phenolic compounds and oenological extracts against potential respiratory pathogensLett. Appl. Microbiol.54(6)557-563(2012) 5.Mink, S.N., Jacobs, H., Gotes, J., et al.Ethyl gallate, a scavenger of hydrogen peroxide that inhibits lysozyme-induced hydrogen peroxide signaling in vitro, reverses hypotension in canine septic shockJ. Appl. Physiol.110(2)359-374(2011)
Kinase experiment: | The expression of apoptosis-related proteins (caspases-8, -9, -3; AIF; Endo G; Bid; Bax; and Bcl-2) in HL-60 cells is determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of lysates followed by western blotting. For this, HL-60 cells (1.5×106) are treated with 50 μM or 75 μM Ethyl gallate for 6 h, 12 h, or 24 h. Total cell lysates are obtained by resuspending cells in ice-cold radioimmunoprecipitation assay (RIPA) buffer for 30 min followed by centrifugation. Protein concentration is determined using a NanoDrop spectrophotometer. Aliquots of lysates (100 μg protein equivalents) are resolved by 12% SDS-PAGE and transferred onto nitrocellulose membranes[1]. |
Cell experiment: | HL-60 cells (1×106) are treated with 50 μM or 75 μM Ethyl gallate for 24 h or 48 h at 37°C. Cells are then harvested by centrifugation and fixed in 70% ethanol at 4°C for 24 h. Fixed cells are resuspended in PBS containing 40 μg/mL Propidium iodide (PI), 100 μg/mL RNase A, and 0.1% Triton X-100 and incubated in the dark for 30 min at room temperature. Cell cycle distribution is analyzed by flow cytometry on a FACSCalibur. To investigate apoptotic cells, HL-60 cells (1×106) incubated with different concentration of 50 μM, 75 μM and 100 μM Ethyl gallate for 24 h or 48 h at 37°C, and then DAPI staining is conducted. The cells are photographed using a fluorescence microscopy[1]. |
Animal experiment: | Forty eight female albino Wistar rats of six to eight weeks old are used and divided into eight groups based on their body weights. Group 1 rats serve as control receiving 1.0 mL of the vehicle (0.1% ethanol); Group 2 rats receive A. nilotica (L.) leaf extract (250 mg/kg body weight); Group 3 rats receive A. nilotica (L.) leaf extract (500 mg/kg body weight); Group 4 rats receive A. nilotica (L.) leaf extract (1000 mg/kg body weight); Group 5 rats receive A. nilotica (L.) leaf extract (2000 mg/kg body weight); Group 6 rats receive Ethyl gallate (5 mg/kg body weight); Group 7 rats receive Ethyl gallate (10 mg/kg body weight); Group 8 rats receive Ethyl gallate (20 mg/kg body weight). Body weights are recorded on 0th and 14th day for each group and all rats are decapitated after an overnight fast[2]. |
References: [1]. Kim WH, et al. Ethyl gallate induces apoptosis of HL-60 cells by promoting the expression of caspases-8, -9, -3, apoptosis-inducing factor and endonuclease G. Int J Mol Sci. 2012;13(9):11912-22. | |
| Cas No. | 831-61-8 | SDF | |
| 别名 | 没食子酸乙酯 | ||
| Canonical SMILES | O=C(OCC)C1=CC(O)=C(O)C(O)=C1 | ||
| 分子式 | C9H10O5 | 分子量 | 198.17 |
| 溶解度 | DMSO : 150 mg/mL (756.93 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.0462 mL | 25.2309 mL | 50.4617 mL |
| 5 mM | 1.0092 mL | 5.0462 mL | 10.0923 mL |
| 10 mM | 0.5046 mL | 2.5231 mL | 5.0462 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet