Evans Blue tetrasodium salt
(Synonyms: 伊文思蓝; Direct Blue 53; C.I. 23860) 目录号 : GC13947
Evans Blue tetrasodium salt是一种高效的囊泡谷氨酸吸收抑制剂（IC50=87nM）。
Cas No.:314-13-6
Sample solution is provided at 25 µL, 10mM.
Evans Blue tetrasodium salt is a highly potent inhibitor of the vesicular glutamate uptake (IC50=87nM)[1]. Evans Blue is widely used in biomedicine including the estimation of blood volume, the assessment of vascular permeability to macromolecules, the detection of lymph nodes, and the location of the tumor lesions[2].
Evans Blue (0-100μM, 1h) potently reduced Epsilon-toxin (ETX)-induced hemolysis (EC50: ~1.2μM)[3].
Evans Blue (2%, 4ml/kg; i.v.; 50min) combined with FITC-Dextran (2000kDa) can be used to quantify blood-brain barrier (BBB) leakage in specific regions of mice with lipopolysaccharide-induced BBB disruption and in apoE-/- mice[4].
References:
[1] Roseth S, Fykse EM, Fonnum F. Uptake of l‐glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. Journal of neurochemistry. 1995 Jul;65(1):96-103.
[2] Yao L, Xue X, Yu P, et al. Evans blue dye: a revisit of its applications in biomedicine. Contrast media & molecular imaging. 2018;2018(1):7628037.
[3] Gao J, Xin W, Huang J, et al. Hemolysis in human erythrocytes by Clostridium perfringens epsilon toxin requires activation of P2 receptors. Virulence. 2018 Dec 31;9(1):1601-14.
[4] Xu Y, He Q, Wang M, et al. Quantifying blood-brain-barrier leakage using a combination of evans blue and high molecular weight FITC-Dextran. Journal of neuroscience methods. 2019 Sep 1;325:108349.
Evans Blue tetrasodium salt是一种高效的囊泡谷氨酸吸收抑制剂（IC50=87nM）[1]。Evans Blue在生物医学领域有着广泛的应用，包括血容量的估计、血管对大分子通透性的评估、淋巴结的检测、肿瘤病变的定位等[2]。
Evans Blue tetrasodium salt（0-100μM，1小时）可有效降低ε毒素（ETX）引起的溶血（EC50：~1.2μM）[3]。
Evans Blue（2%，4ml/kg；静脉注射；50分钟）与FITC-葡聚糖（2000kDa）结合可用于量化脂多糖诱导血脑屏障（BBB）破坏的小鼠和特定区域的血脑屏障渗漏的apoE-/-小鼠[4]。
| Cell experiment [1]: | |
Cell lines | Erythrocytes isolated from healthy volunteers |
Preparation Method | Human blood was collected by venopuncture from healthy volunteers using evacuated blood collection tubes containing EDTA-2K. The blood of mice, rats, sheep, bovine, rabbits, monkeys, horses, dogs and goats were collected, then the erythrocytes were washed three times in 0.01M PBS (1,000g, 4°C, 5min). The “buffy coat” was removed and isolated erythrocytes were packed by centrifugation at 1,000g for 10min at 4°C. Washed erythrocytes were resuspended in 0.01M PBS to produce a 5% erythrocyte solution. The hemolytic activity was measured spectrophotometrically at 540nm. In the hemolytic assay, purified recombinant ETX (rETX) (0 ~ 33μM) and increasing concentrations of Evans Blue was added to the 5% red blood cell solution (final concentration of 3.3%) and incubated up to 1h at 37°C with constant swirling (300rpm). Then the 1.5ml tubes were centrifuged at 1,000g for 10min at 4°C, and the optical density at 540nm of the supernatants was determined as a measure of the released hemoglobin. |
Reaction Conditions | 0-100μM, 1h |
Applications | Evans Blue potently reduced Epsilon-toxin (ETX)-induced hemolysis (EC50: ~1.2μM). |
| Animal experiment [2]: | |
Animal models | Blood-brain barrier mouse model |
Preparation Method | Eight C57BL/6J mice were intraperitoneally injected with 1mg/ml LPS (4ml/kg, diluted in normal saline) to induce BBB disruption. Another eight C57BL/6J mice were intraperitoneally injected with normal saline (4ml/kg) as control (LPS-control). Six hours after the injection, mice were anesthetized with 1.5% pentobarbital sodium (3ml/kg, i.p.), then cannulated with a 26 Ga catheter through the tail vein. 2% Evans Blue (4ml/kg, diluted in normal saline) was injected through the catheter. 50min after EB circulation, 6mg/ml FITC-Dextran (4ml/kg, diluted in Normal Saline) was injected through the catheter and circulated for 10min. |
Dosage form | 2%, 4ml/kg; i.v.; 50min |
Applications | Evans Blue combined with FITC-Dextran (2000kDa) can be used to quantify blood-brain barrier (BBB) leakage in specific regions of mice with lipopolysaccharide-induced BBB disruption and in apoE-/- mice. |
References: | |
| Cas No. | 314-13-6 | SDF | |
| 别名 | 伊文思蓝; Direct Blue 53; C.I. 23860 | ||
| 化学名 | sodium (6Z,6'Z)-6,6'-(2,2'-(3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(hydrazin-2-yl-1-ylidene))bis(4-amino-5-oxo-5,6-dihydronaphthalene-1,3-disulfonate) | ||
| Canonical SMILES | O=C1C(C(C=C/C1=N/NC(C(C)=C2)=CC=C2C3=CC=C(C(C)=C3)N/N=C4C(C(C(C=C\4)=C5S([O-])(=O)=O)=C(C(S([O-])(=O)=O)=C5)N)=O)=C6S([O-])(=O)=O)=C(C(S([O-])(=O)=O)=C6)N.[Na+].[Na+].[Na+].[Na+] | ||
| 分子式 | C34H24N6Na4O14S4 | 分子量 | 960.82 |
| 溶解度 | ≥ 192.4 mg/mL in DMSO, ≥ 213.8 mg/mL in Water | 储存条件 | Store at RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.0408 mL | 5.2039 mL | 10.4078 mL |
| 5 mM | 0.2082 mL | 1.0408 mL | 2.0816 mL |
| 10 mM | 0.1041 mL | 0.5204 mL | 1.0408 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet