Farnesene (mixture of isomers)
目录号 : GC64881Farnesene (mixture of isomers) 是一种含有异构体混合物的 Farnesene。Farnesene 是一种倍半萜烯,并且是草食动物诱导的植物挥发物 (HIPV)。Farnesene 对许多植物物种的抗虫性具有重要影响。
Cas No.:125037-13-0
Sample solution is provided at 25 µL, 10mM.
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Farnesene (mixture of isomers) is a farnesene with mixture of isomers. Farnesene is a herbivore-induced plant volatile (HIPV). Farnesene has an important effect on insect resistance in many plant species[1].
[1]. Xuewen Wang, et al. Formation of α-Farnesene in Tea ( Camellia sinensis) Leaves Induced by Herbivore-Derived Wounding and Its Effect on Neighboring Tea Plants. Int J Mol Sci. 2019 Aug 25;20(17):4151.
Cas No. | 125037-13-0 | SDF | Download SDF |
分子式 | C15H24 | 分子量 | 204.35 |
溶解度 | DMSO : 41.67 mg/mL (203.91 mM; ultrasonic and warming and heat to 60°C) | 储存条件 | Store at -20°C |
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1 mg | 5 mg | 10 mg | |
1 mM | 4.8936 mL | 24.4678 mL | 48.9356 mL |
5 mM | 0.9787 mL | 4.8936 mL | 9.7871 mL |
10 mM | 0.4894 mL | 2.4468 mL | 4.8936 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
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一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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Neuroprotective effects of Farnesene against hydrogen peroxide-induced neurotoxicity in vitro
Cell Mol Neurobiol 2014 Jan;34(1):101-11.PMID:24105026DOI:10.1007/s10571-013-9991-y.
Oxidative stress is highly damaging to cellular macromolecules and is also considered a main cause of the loss and impairment of neurons in several neurodegenerative disorders. Recent reports indicate that Farnesene (FNS), an acyclic sesquiterpene, has antioxidant properties. However, little is known about the effects of FNS on oxidative stress-induced neurotoxicity. We used hydrogen peroxide (H2O2) exposure for 6 h to model oxidative stress. Therefore, this experimental design allowed us to explore the neuroprotective potential of different FNS isomers (α-FNS and β-FNS) and their mixture (Mix-FNS) in H2O2-induced toxicity in newborn rat cerebral cortex cell cultures for the first time. For this aim, both MTT and lactate dehydrogenase assays were carried out to evaluate cell viability. Total antioxidant capacity (TAC) and total oxidative stress (TOS) parameters were used to assess oxidative alterations. In addition to determining of 8-hydroxy-2-deoxyguanosine (8-OH-dG) levels in vitro, the comet assay was also performed for measuring the resistance of neuronal DNA to H2O2-induced challenge. Our results showed that survival and TAC levels of the cells decreased, while TOS, 8-OH-dG levels and the mean values of the total scores of cells showing DNA damage (comet assay) increased in the group treated with H2O2 alone. But pretreatment of FNS suppressed the cytotoxicity, genotoxicity and oxidative stress, which were increased by H2O2 in clear type of isomers and applied concentration-dependent manners. The order of antioxidant effectiveness for modulating H2O2-induced oxidative stress-based neurotoxicity and genotoxicity is as β-FNS > Mix-FNS > α-FNS.
An odorant receptor and glomerulus responding to Farnesene in Helicoverpa assulta (Lepidoptera: Noctuidae)
Insect Biochem Mol Biol 2019 Dec;115:103106.PMID:30468768DOI:10.1016/j.ibmb.2018.11.006.
Terpenoids emitted from herbivore-damaged plants were found to play an important role in regulating tritrophic interactions. How herbivores and their natural enemies perceive terpenoids has not been thoroughly elucidated to date. Using in vivo calcium imaging, we found in this study that Farnesene activates one glomerulus in the antennal lobe of female Helicoverpa assulta. The response induced by a mixture of Farnesene isomers is stronger than that elicited by E-β-farnesene alone. In the Xenopus oocyte expression system, HassOR23/ORco is narrowly tuned to Farnesene isomers and compounds with similar structures. Finally, the behavioral studies showed that the Farnesene isomers have an inhibitory effect on oviposition of female H. assulta, but have an attractive effect on host searching of Campoletis chlorideae, the key endoparasitoid of H. assulta larvae. These results demonstrate that Farnesene isomers are encoded by a labeled-line mode in the olfactory system of female H. assulta, suggesting that Farnesene as a chemical signal from plants has important behavioral relevance and evolutionary implications in the tritrophic context.
(3S,6E)-nerolidol-mediated rendezvous of Cyclocephala paraguayensis beetles in bottle gourd flowers
PLoS One 2020 Dec 23;15(12):e0235028.PMID:33362256DOI:10.1371/journal.pone.0235028.
Cyclocephalini beetles of the genus Cyclocephala (Coleoptera: Melolonthidae: Dynastinae) use flowers of some plants as food, shelter, and mating sites. However, little is known about floral scent chemistry involved in this interaction. Here we show that a sesquiterpene alcohol mediates attraction of Cyclocephala paraguayensis Arrow, on bottle gourd flowers, Lagenaria siceraria (Cucurbitaceae). Both males and females started to aggregate on the flowers at twilight; after that, mating began and remained for the entire night. GC-FID/EAD analysis of the L. siceraria floral scent collected in the field revealed that only the major constituent of the airborne volatiles elicited electroantennographic responses on male and female antennae of C. paraguayensis. This compound was identified as (3S,6E)-nerolidol, which was tested in two field trapping trials in Brazil. In the first bioassay, traps baited with nerolidol (mix of isomers) captured significantly more adult C. paraguayensis than control traps. In the second field trial, catches in traps baited with a mixture of isomers or enantiopure nerolidol were significantly higher than captures in control traps, but the treatments did not differ significantly. Analysis from the gut content of adult C. paraguayensis showed the presence of pollen, suggesting that they also use bottle gourd flowers for their nourishment. Taken together, these results suggest that (3S,6E)-nerolidol plays an essential role in the reproductive behavior of C. paraguayensis by eliciting aggregation, mating, and feeding.