Fast-TRFS
目录号 : GC61558Fast-TRFS是硫氧还蛋白还原酶(TrxR)的选择性超快荧光探针。Fast-TRFS可用于对活细胞中的TrxR活性进行成像。
Cas No.:2966536-18-3
Sample solution is provided at 25 µL, 10mM.
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Fast-TRFS is a selective and superfast fluorogenic probe of thioredoxin reductase (TrxR). Fast-TRFS can be used for imaging TrxR activity in live cells[1].
Fast-TRFS reaches the maximal fluorescence signal within 1 min incubated with TCEP, and within 5 min incubated with the TrxR enzyme[1].Fast-TRFS (10 μM; 2-60 min) appears the blue fluorescence signal within 2 min in HeLa cells[1].
[1]. Li X, et, al. A fast and specific fluorescent probe for thioredoxin reductase that works via disulphide bond cleavage. Nat Commun. 2019 Jun 21;10(1):2745.
Cas No. | 2966536-18-3 | SDF | |
Canonical SMILES | O=C1OC(C=C(C=C2)NC(NC3CSSC3)=O)=C2C(C(F)(F)F)=C1 | ||
分子式 | C14H11F3N2O3S2 | 分子量 | 376.37 |
溶解度 | DMSO : 100 mg/mL (265.70 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
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A fast and specific fluorescent probe for thioredoxin reductase that works via disulphide bond cleavage
Nat Commun 2019 Jun 21;10(1):2745.PMID:31227705DOI:10.1038/s41467-019-10807-8.
Small molecule probes are indispensable tools to explore diverse cellular events. However, finding a specific probe of a target remains a high challenge. Here we report the discovery of Fast-TRFS, a specific and superfast fluorogenic probe of mammalian thioredoxin reductase, a ubiquitous enzyme involved in regulation of diverse cellular redox signaling pathways. By systematically examining the processes of fluorophore release and reduction of cyclic disulfides/diselenides by the enzyme, structural factors that determine the response rate and specificity of the probe are disclosed. Mechanistic studies reveal that the fluorescence signal is switched on by a simple reduction of the disulfide bond within the probe, which is in stark contrast to the sensing mechanism of published probes. The favorable properties of Fast-TRFS enable development of a high-throughput screening assay to discover inhibitors of thioredoxin reductase by using crude tissue extracts as a source of the enzyme.
A Fluorescent Probe to Detect Quick Disulfide Reductase Activity in Bacteria
Antioxidants (Basel) 2022 Feb 13;11(2):377.PMID:35204259DOI:10.3390/antiox11020377.
The Trx and Grx systems, two disulfide reductase systems, play critical roles in various cell activities. There are great differences between the thiol redox systems in prokaryotes and mammals. Though fluorescent probes have been widely used to detect these systems in mammalian cells. Very few methods are available to detect rapid changes in the redox systems of prokaryotes. Here we investigated whether Fast-TRFS, a disulfide-containing fluorescent probe utilized in analysis of mammalian thioredoxin reductase, could be used to detect cellular disulfide reducibility in bacteria. Fast-TRFS exhibited good substrate qualities for both bacterial thioredoxin and GSH-glutaredoxin systems in vitro, with Trx system having higher reaction rate. Moreover, the Fast-TRFS was used to detect the disulfide reductase activity in various bacteria and redox-related gene null E. coli. Some glutaredoxin-deficient bacteria had stronger fast disulfide reducibility. The Trx system was shown to be the predominant disulfide reductase for fast disulfide reduction rather than the Grx system. These results demonstrated that Fast-TRFS is a viable probe to detect thiol-dependent disulfide reductases in bacteria. It also indicated that cellular disulfide reduction could be classified into fast and slow reaction, which are predominantly catalyzed by E. coli Trx and Grx system, respectively.