FD-1080
目录号 : GC64721
FD-1080是在第二近红外区II（NIR-II）区域中具有激发和发射光的荧光团（Ex=1064nm, Em=1080nm），可用于体内成像。
Cas No.:1151666-58-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >90.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
本方案仅提供一个指导，请根据您的具体需要进行修改。
1. 溶液配制
(1)储存液：将低温保存的固体平衡至室温，使用DMSO溶解FD-1080，配置浓度为10mM的储存液。
注意:建议将未使用的储存液分装后在-20℃或-80°C避光保存，避免反复冻融。
(2)工作液：用PBS稀释储存液，配制浓度为2-10μM的工作液。0.2μm滤膜过滤除菌。
注意：最佳的工作浓度请根据实际情况调整或参阅文献自行设置梯度浓度进行摸索。工作液必须现配现用。
2. 细胞实验
（1）悬浮细胞：离心收集细胞，加入PBS洗涤两次，每次5min。贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入PBS 洗涤两次，每次5min。
（2）加入1mL FD-1080工作液，室温孵育10-30min。
（3）400g，4℃离心3-4min，弃去上清。
（4）加入PBS洗涤细胞两次，每次5min。
（5）用1mL无血清培养基或PBS重悬细胞后，在荧光显微镜或流式细胞仪下检测。
3. 活体注射
（1）将200μL 80μM FD-1080工作液静脉注射至小鼠体内，10-20min后进行体内成像分析。
FD-1080 is a fluorophore with excitation and emission light in the second near-infrared region II (NIR-II) (Ex=1064nm, Em=1080nm) that can be used for in vivo imaging[1]. FD-1080 has high quantum yield, deep tissue penetration depth, superior spatial resolution, outstanding signal-to-noise ratio, and dynamic imaging capabilities[1]. The quantum yield of FD-1080 in FBS solvent can be increased to 5.94%[2]. FD-1080 can not only achieve non-invasive and high-resolution bioimaging of deep tissue vascular and cerebral vessels in the hind limb, but also quantify respiratory rate based on dynamic imaging of craniocaudal respiratory movement in the liver of awake and anesthetized mice[3, 4].
References:
[1] Li B, Lu L, Zhao M, et al. An efficient 1064 nm NIR‐II excitation fluorescent molecular dye for deep‐tissue high‐resolution dynamic bioimaging[J]. Angewandte Chemie, 2018, 130(25): 7605-7609.
[2] Zhou H, Xiao Y, Hong X. New NIR-II dyes without a benzobisthiadiazole core[J]. Chinese Chemical Letters, 2018, 29(10): 1425-1428.
[3] He S, Cheng Z. Advancements of second near-infrared biological window fluorophores: Mechanism, synthesis, and application in vivo[J]. Fluorescent Imaging in Medicinal Chemistry, 2020: 81-123.
[4] Ding F, Fan Y, Sun Y, et al. Beyond 1000 nm Emission Wavelength: Recent Advances in Organic and Inorganic Emitters for Deep‐Tissue Molecular Imaging[J]. Advanced Healthcare Materials, 2019, 8(14): 1900260.
FD-1080是在第二近红外区II（NIR-II）区域中具有激发和发射光的荧光团（Ex=1064nm, Em=1080nm），可用于体内成像[1]。FD-1080具有高量子产率、深组织穿透深度、上级空间分辨率、突出的信噪比和动态成像能力[1]。FD-1080在FBS溶剂中的量子产率可提高至5.94%[2]。FD-1080不仅能够实现无创高分辨率的后肢深部组织血管和脑血管生物成像，而且能够基于清醒和麻醉小鼠肝脏呼吸头尾向运动的动态成像来量化呼吸频率[3, 4]。
| Cas No. | 1151666-58-8 | SDF | Download SDF |
| 分子式 | C40H38ClN2NaO6S2 | 分子量 | 765.31 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.3067 mL | 6.5333 mL | 13.0666 mL |
| 5 mM | 0.2613 mL | 1.3067 mL | 2.6133 mL |
| 10 mM | 0.1307 mL | 0.6533 mL | 1.3067 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
An Efficient 1064 nm NIR-II Excitation Fluorescent Molecular Dye for Deep-Tissue High-Resolution Dynamic Bioimaging
Angew Chem Int Ed Engl 2018 Jun 18;57(25):7483-7487.PMID:29493057DOI:10.1002/anie.201801226.
A small-molecule fluorophore FD-1080 with both excitation and emission in the NIR-II region has been successfully synthesized for in vivo imaging. A heptamethine structure is designed to shift the absorption and emission into NIR-II region. Sulphonic and cyclohexene groups are introduced to enhance its water solubility and stability. The quantum yield of FD-1080 is 0.31 %, and can be increased to 5.94 % after combining with fetal bovine serum (FBS). Significantly, 1064 nm NIR-II excitation was demonstrated with the high tissue penetration depth and superior imaging resolution compared to previously reported NIR excitation from 650 nm to 980 nm. FD-1080 is not only capable of realizing non-invasive high-resolution deep-tissue hindlimb vasculature and brain vessel bioimaging, but also quantifying the respiratory rate based on the dynamic imaging of respiratory craniocaudal motion of the liver for the awake and anaesthetized mouse.
J-Aggregates of Cyanine Dye for NIR-II in Vivo Dynamic Vascular Imaging beyond 1500 nm
J Am Chem Soc 2019 Dec 11;141(49):19221-19225.PMID:31746598DOI:10.1021/jacs.9b10043.
Light in the second near-infrared window, especially beyond 1500 nm, shows enhanced tissue transparency for high-resolution in vivo optical bioimaging due to decreased tissue scattering, absorption, and autofluorescence. Despite some inorganic luminescent nanoparticles have been developed to improve the bioimaging around 1500 nm, it is still a great challenge to synthesize organic molecules with the absorption and emission toward this region. Here, we present J-aggregates with 1360 nm absorption and 1370 nm emission formed by self-assembly of amphiphilic cyanine dye FD-1080 and 1,2-dimyristoyl-sn-glycero-3-phosphocholine. Molecular dynamics simulations were further employed to illustrate the self-assembly process. Superior spatial resolution and high signal-to-background ratio of J-aggregates were demonstrated for noninvasive brain and hindlimb vasculature bioimaging beyond 1500 nm. The efficacy evaluation of the clinically used hypotensor is successfully achieved by high-resolution in vivo dynamic vascular imaging with J-aggregates.