Fetuin, Fetal Bovine Serum
(Synonyms: 胎球蛋白) 目录号 : GC60166
Fetuin, Fetal Bovine Serum是一种来自胎牛血清的糖蛋白，通常是许多无血清培养基的重要补充。
Cas No.:9014-81-7
Sample solution is provided at 25 µL, 10mM.
Fetuin, Fetal Bovine Serum is a glycoprotein from fetal bovine serum, which is often an important supplement to many serum-free media. Bovine fetuin preparation has inhibited trypsin activity and promote cellular attachment, growth, and differentiation in many different culture systems [1]. Fetuins are established as a family within the cystatin superfamily [2].
Fetuin, Fetal Bovine Serum had activity on the S3 strain of HeLa cells for promoting cell attachment and stretching (flattening) on the glass [3]. Fetuin, Fetal Bovine Serum preparations stimulated the growth of a variety of mammalian cells from different species. Fetuin, Fetal Bovine Serum (30- 960 µg/ml) stimulated the growth of Hela cell [4]. The nuclei of the LNCaP cells that had been incubated with the Fetuin, Fetal Bovine Serum showed the characteristics of apoptosis [5].
Fetuin, Fetal Bovine Serum enhanced survival rate by up to 141% in the P388 leukemia model. In the PC-3 prostate cancer animal model, fetuin (50 mg/kg) seems to completely inhibit the formation of prostate cancer in mice [5].
References:
[1]. Nie Z. Fetuin: its enigmatic property of growth promotion[J]. American Journal of Physiology-Cell Physiology, 1992, 263(3): C551-C562.
[2]. Brown W M, Dziegielewska K M, Saunders N R, et al. Fetuin-an old friend revisited[J]. Bioessays, 1992, 14(11): 749-755.
[3]. Fisher H W, Puck T T, Sato G. Molecular growth requirements of single mammalian cells: The action of fetuin in promoting cell attachment to glass[J]. Proceedings of the National Academy of Sciences, 1958, 44(1): 4-10.
[4]. Marr A G M, Owen J A, Wilson G S. Studies on the growth-promoting glycoprotein fraction of foetal calf serum[J]. Biochimica et Biophysica Acta, 1962, 63(2): 276-285.
[5]. Yu C L, Tsai M H. Fetal fetuin selectively induces apoptosis in cancer cell lines and shows anti-cancer activity in tumor animal models[J]. Cancer letters, 2001, 166(2): 173-184.
| Animal experiment [1]: | |
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Animal models |
Male nude mice |
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Preparation Method |
The prostate adenocarcinoma cell PC-3 (2 million cells) were injected on the upper half of the dorsal thorax of the mice. The tumors were allowed to grow for 6 weeks. The mice in the control group (no treatment) received 0.1 ml saline intraperitoneally for 5 days and the mice in the treated group received 50 mg/kg Fetuin, Fetal Bovine Serum. Treatment started the day after tumor inoculation for 5 days. Six weeks after the inoculation of tumor, mice were killed and tumors were removed and weighed. |
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Dosage form |
Intraperitoneal injection, 50 mg/kg/d for 5d |
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Applications |
Tumors (average weight 325 mg) developed in 13 of 13 mice in the control group (treated with saline), none of the nine mice treated with fetuin (50 mg/kg) developed any tumor. |
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References: [1]: Yu C L, Tsai M H. Fetal fetuin selectively induces apoptosis in cancer cell lines and shows anti-cancer activity in tumor animal models[J]. Cancer letters, 2001, 166(2): 173-184. |
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| Cas No. | 9014-81-7 | SDF | |
| 别名 | 胎球蛋白 | ||
| 分子式 | 分子量 | ||
| 溶解度 | H2O : 50 mg/mL (Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet