FGF 18 Mouse
目录号 : GP20239Fibroblast Growth Factor-18 Mouse Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Fibroblast growth factor 18; FGF-18; zFGF5; Fgf18; D130055P09Rik. | ||
| Amino Acid Sequence | EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQAELQK PFKYTTVTKR SRRIRPTHPG. | ||
| Solubility | It is recommended to reconstitute the lyophilized FGF-18 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized FGF-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution FGF-18 should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is< 0.5ng/ml, corresponding to a specific activity of> 2.0б┴106 units/mg. | ||
| Formulation | FGF-18 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. | ||
Fibroblast growth factor 18 (FGF18) is a member of the large FGF family which has at least 23 members. FGF18 is a heparin binding growth factor with a core 120 amino acid FGF domain which allows for a common tertiary structure. FGFs are expressed in the course of the embryonic development and in restricted adult tissues. FGF-18 is an indispensable regulator of long bone and calvarial development. FGF-18 signals via FGFR 1c, 2c, 3c, and 4.
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is< 0.5ng/ml, corresponding to a specific activity of> 2.0б┴106 units/mg.
Lyophilized FGF-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution FGF-18 should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.