FGF 2 Human (147 a.a.)
目录号 : GP20212Fibroblast Growth Factor Basic 147 a.a. Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Prostatropin; HBGH-2; HBGF-2; FGF-2; FGF-b. | ||
| Amino Acid Sequence | MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS. | ||
| Solubility | It is recommended to reconstitute the lyophilized FGF-B in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized basic-FGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution FGFb should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50, Calculated by the dose- dependent proliferation of mouse BALB/c 3T3 cells is 22.5pg/ml corresponding to a specific activity of 4.4x107 units/mg. | ||
| Formulation | The bFGF was lyophilized from a sterile filtered solution containing 10mM sodium phosphate, 75mM sodium chloride, pH 7.5. | ||
FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
The ED50, Calculated by the dose- dependent proliferation of mouse BALB/c 3T3 cells is 22.5pg/ml corresponding to a specific activity of 4.4x107 units/mg.
Lyophilized basic-FGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution FGFb should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.