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FGF20 Human

目录号 : GP20244

成纤维细胞生长因子 20 人重组体

FGF20 Human Chemical Structure

规格 价格 库存 购买数量
3μg
¥840.00
5-10工作日
15μg
¥2,030.00
5-10工作日
1mg
¥49,140.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

Description

FGF20 Human Recombinant (1-211) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 217 amino acids and having a molecular mass of 24kDa.The FGF-20 is fused to a 6 amino acid His tag [HHHHHH] at N-terminus and purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 97.0% as determined by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms Fibroblast Growth Factor 20; FGF-20; RHDA2; FGF20.
Amino Acid Sequence MHHHHHHAPL AEVGGFLGGL EGLGQQVGSH FLLPPAGERP PLLGERRSAA ERSARGGPGA AQLAHLHGIL RRRQLYCRTG FHLQILPDGS VQGTRQDHSL FGILEFISVA VGLVSIRGVD SGLYLGMNDK GELYGSEKLT SECIFREQFE ENWYNTYSSN IYKHGDTGRR YFVALNKDGT PRDGARSKRH QKFTHFLPRP VDPERVPELY KDLLMYT.
Solubility It is recommended to reconstitute the lyophilized FGF-20 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized FGF20 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution FGF-20 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity The ED50, as measured in a proliferation assay using mouse NR6R-3T3 cells, is less than 2.5ng/ml.
Formulation Lyophilized from a 0.2µm filtered solution in MOPS, (NH4)2SO4, DTT and EDTA.

Introduction

Fibroblast growth factor 20 (FGF20) belongs to the FGF gene family and member of FGF-9 subfamily (based upon its structure). Human FGF20 has several receptors which include FGF R1c, FGF R2c, FGF R3b, FGF R3c and FGF R4. FGF20 is expressed a various cells, including dopaminergic neurons, fibroblasts, keratinocytes and breast epithelium, and numerous sites in the fetus.

Biological Activity

The ED50, as measured in a proliferation assay using mouse NR6R-3T3 cells, is less than 2.5ng/ml.

Stability

Lyophilized FGF20 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution FGF-20 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

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