FGF23 Human
目录号 : GP20253Fibroblast Growth Factor-23 Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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Fibroblast Growth Factor-23 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing a total of 228 amino acids and having a molecular mass of 22.5kDa. The FGF-23 is and purified by chromatographic techniques.
| Purity | Greater than 95.0% as determined by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered white lyophilized powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Tumor-derived hypophosphatemia-inducing factor; HYPF; ADHR; HPDR2; PHPTC; FGF23; FGF-23; Fibroblast Growth Factor-23. | ||
| Amino Acid Sequence | MYPNASPLLG SSWGGLIHLY TATARNSYHL QIHKNGHVDG APHQTIYSAL MIRSEDAGFV VITGVMSRRY LCMDFRGNIF GSHYFDPENC RFQHQTLENG YDVYHSPQYH FLVSLGRAKR AFLPGMNPPP YSQFLSRRNE IPLIHFNTPI PRRHTRSAED DSERDPLNVL KPRARMTPAP ASCSQELPSA EDNSPMASDP LGVVRGGRVN THAGGTGPEG CRPFAKFI. | ||
| Solubility | It is recommended to reconstitute the lyophilized FGF-23 in sterile 18M-cm H2O not less than 100g/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized FGF-23 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution FGF-23 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The biological activity of FGF-23 was measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.05-0.5 μg/ml in the presence of 5 μg/ml of Recombinant Mouse Klotho and 10 μg/ml of heparin. | ||
| Formulation | The FGF-23 protein (0.5mg/ml) was lyophilized from a 0.2m filtered concentrated solution in PBS, pH 7.4. | ||
FGF-23 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-23 inhibits renal tubular phosphate transport. The FGF-23gene was identified by its mutations associated with autosomal dominant hypophosphatemic rickets (ADHR), an inherited phosphate wasting disorder. Abnormally high level expression of FGF-23 was found in oncogenic hypophosphatemic osteomalacia (OHO), a phenotypically similar disease caused by abnormal phosphate metabolism. FGF-23 mutations have also been shown to cause familial tumoral calcinosis with hyperphosphatemia.
The biological activity of FGF-23 was measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.05-0.5 μg/ml in the presence of 5 μg/ml of Recombinant Mouse Klotho and 10 μg/ml of heparin.
Lyophilized FGF-23 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution FGF-23 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.