FHZ
目录号 : GC30699
FHZ是一个荧光探针。
Cas No.:1883737-63-0
Sample solution is provided at 25 µL, 10mM.
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FHZ is a fluorescent probe.
After loaded with probe FHZ and treated with HClO and H2O2/EDTA-Fe2+ in order, HeLa cells display the bright fluorescences from both cyan and green channels. FHZ can give out two different fluorescent signals in the presence of both •OH and HClO, suggesting the synchronous discrimination of •OH and HClO by a dual-channel detection model with two exciting wavelengths. Probe FHZ shows very high specificity to the detections of •OH and HClO with the excitations at 410 and 490 nm, respectively. The probe FHZ can efficiently enter the cellular mitochondria and exhibit the differentiable/visual capabilities to the endogenous •OH and HClO by the dual fluorescent responses[1].
Probe FHZ can rapidly be absorbed into the blood circulation system from the zebrafish intestine, and spread out whole zebrafish tissues, and keep its stability in the blood, organs and tissues in the absence of ROS. The probe can keep its stability in biological environments and only selectively react with •OH and HClO species[1].
[1]. Zhang R, et al. Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe. J Am Chem Soc. 2016 Mar 23;138(11):3769-78.
Cell experiment: | HeLa cells and RAW264.7 macrophages are cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) supplied with 10% fetal bovine serum (FBS) and 1% antibotics (penicillin and streptomycin) at 37°C in humidified incubator containing 5% CO2. The cells are seeded into glass-bottomed dishes and cultured for 24 h. Subsequently, the cells are incubated with FHZ for 30 min at 37°C and then washed with PBS buffer three times. Each treatment of cells with H2O2, EDTA-Fe2+, HClO or scavengers kept 30 min[1]. |
Animal experiment: | Wild type zebrafish is used in this study. Seven-day old fertilized zebrafish embryos are cultured in 50 μM FHZ for 30 min, and then the zebrafish embryos are transferred to fresh water. A FHZ-loaded zebrafish is fixed under confocal microscope using 2% agarose gel to keep its living state for fluorescent imaging. In order to observe the release of •OH in fresh wound, the ventral fin of the FHZ-loaded zebrafish is carefully cut a small wound using a blade. After raised for 20 min in water, the wound of injured zebrafish is imaged using confocal microscope[1]. |
References: [1]. Zhang R, et al. Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe. J Am Chem Soc. 2016 Mar 23;138(11):3769-78. | |
| Cas No. | 1883737-63-0 | SDF | |
| Canonical SMILES | O=C1N(N)C2(C3=C(OC4=C2C=CC(OCCOCCOCCO)=C4)C=C(O)C=C3)C5=C1C=CC=C5 | ||
| 分子式 | C26H26N2O7 | 分子量 | 478.49 |
| 溶解度 | DMSO : 100 mg/mL (208.99 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0899 mL | 10.4495 mL | 20.8991 mL |
| 5 mM | 0.418 mL | 2.0899 mL | 4.1798 mL |
| 10 mM | 0.209 mL | 1.045 mL | 2.0899 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet