FIN56
目录号 : GC30039
FIN56 是一种新型的铁死亡诱导剂，它通过增加 GPX4 的降解来触发铁死亡 。
Cas No.:1083162-61-1
Sample solution is provided at 25 µL, 10mM.
FIN56, a novel ferroptosis inducer, triggers ferroptosis by increasing the degradation of GPX4 [1,4]. FIN56 also activates squalene synthase, an enzyme involved in the cholesterol synthesis [2].
FIN56 (0-8.0 μM;24 h) decreased the cell viability of LN229 and U118 cells in a dose-dependent manner. After FIN56 treatment, cell cycle of LN229 and U118 was arrested at GO/G1 phases [3]. FIN56 (1-5 μM;3-24 h) induces autophagy-associated cell death in Bladder cancer (BC) cells[4]. Inhibition of Gpx4 by FIN56 (0-20μM) abolished the protective effects of NAC on HG-induced ferroptosis[5]. the protein expression of salusin β was upregulated by ferroptosis activators, such as FIN56. Pretreatment with ferrostatin 1 (a ferroptosis inhibitor) prevented the upregulated protein expression of salusin β in HK 2 cells exposed to HG[6]. AS-IV markedly accelerated proliferation, suppressed apoptosis, and reduced ROS and LDH accumulation. The effects of AS-IV on SCI were inhibited by si-TFEB, and this inhibition was further reinforced by the addition of FIN56(5 μM)[7].
FIN56 (30days) decreased tumor volume obviously, FIN56 significantly increased protein levels of 4-HNE[3].
References:
[1]. Shimada K, Skouta R, et,al. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis. Nat Chem Biol. 2016 Jul;12(7):497-503. doi: 10.1038/nchembio.2079. Epub 2016 May 9. PMID: 27159577; PMCID: PMC4920070.
[2]. Gaschler MM, Andia AA, et,al.FINO2 initiates ferroptosis through GPX4 inactivation and iron oxidation. Nat Chem Biol. 2018 May;14(5):507-515. doi: 10.1038/s41589-018-0031-6. Epub 2018 Apr 2. PMID: 29610484; PMCID: PMC5899674.
[3]. Zhang X, Guo Y, et,al. FIN56, a novel ferroptosis inducer, triggers lysosomal membrane permeabilization in a TFEB-dependent manner in glioblastoma. J Cancer. 2021 Sep 13;12(22):6610-6619. doi: 10.7150/jca.58500. PMID: 34659551; PMCID: PMC8517990.
[4]. Lei P, Bai T, et,al.Mechanisms of Ferroptosis and Relations With Regulated Cell Death: A Review. Front Physiol. 2019 Feb 26;10:139. doi: 10.3389/fphys.2019.00139. PMID: 30863316; PMCID: PMC6399426.
[5]. Li Q, Liao J,et,al. NAC alleviative ferroptosis in diabetic nephropathy via maintaining mitochondrial redox homeostasis through activating SIRT3-SOD2/Gpx4 pathway. Free Radic Biol Med. 2022 Jul;187:158-170. doi: 10.1016/j.freeradbiomed.2022.05.024. Epub 2022 May 31. Erratum in: Free Radic Biol Med. 2022 Aug 20;189:1. PMID: 35660452.
[6].Wang WJ, Jiang X,et,al.Salusin?β participates in high glucose?induced HK?2 cell ferroptosis in a Nrf?2?dependent manner. Mol Med Rep. 2021 Sep;24(3):674. doi: 10.3892/mmr.2021.12313. Epub 2021 Jul 23. PMID: 34296310; PMCID: PMC8335735.
[7]. Zhou Y, Li L, et,al.Astragaloside IV ameliorates spinal cord injury through controlling ferroptosis in H2O2-damaged PC12 cells in vitro. Ann Transl Med. 2022 Nov;10(21):1176. doi: 10.21037/atm-22-5196. PMID: 36467371; PMCID: PMC9708485.
FIN56 是一种新型的铁死亡诱导剂，它通过增加 GPX4 的降解来触发铁死亡 [1,4]。 FIN56 还激活角鲨烯合酶，一种参与胆固醇合成的酶[2]。
FIN56（0-8.0 μM；24 小时）以剂量依赖性方式降低 LN229 和 U118 细胞的细胞活力。 FIN56处理后，LN229和U118的细胞周期停滞在GO/G1期[3]。 FIN56（1-5 μM；3-24 小时）在膀胱癌 (BC) 细胞中诱导自噬相关细胞死亡[4]。 FIN56 (0-20μM) 对 Gpx4 的抑制作用消除了 NAC 对 HG 诱导的铁死亡的保护作用[5]。 salusin β 的蛋白表达被 FIN56 等铁死亡激活剂上调。用 ferrostatin 1（一种铁死亡抑制剂）预处理可防止暴露于 HG[6] 的 HK 2 细胞中 salusin β 的蛋白表达上调。 AS-IV 显着加速增殖，抑制细胞凋亡，并减少 ROS 和 LDH 积累。 AS-IV 对 SCI 的影响被 si-TFEB 抑制，并且这种抑制通过添加 FIN56(5 μM)[7] 得到进一步加强。
FIN56（30天）明显缩小肿瘤体积，FIN56显着增加4-HNE蛋白水平[3]。
| Cell experiment [1]: | |
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Cell lines |
LN229 and U118 GBM cell lines |
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Preparation Method |
Cells were plated into 96-well plates and incubated overnight. Different doses of FIN56 (0, 0.1, 0.5, 1.0, 2.0 4.0 and 8.0 µM) was added to wells. 24 h later, CCK-8 solution was added to each well. 2 h later, samples were measured at 450 nm on a microplate reader. |
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Reaction Conditions |
FIN56 (0-8.0 µM);24 h |
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Applications |
FIN56 decreased the cell viability of LN229 and U118 cells in a dose-dependent manner. |
| Animal experiment [1]: | |
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Animal models |
Nude mouse model |
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Preparation Method |
LN229 cells were injected subcutaneously into the right shoulder of 4-week-old nude mice. 2 weeks later, nude mice (n = 10) were divided into two groups, control group and FIN56 treatment group. Subcutaneous tumors were harvested 30 days after treatment. |
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Dosage form |
30 days |
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Applications |
FIN56 decreased tumor volume obviously, FIN56 significantly increased protein levels of 4-HNE. |
|
References: [1]. Zhang X, Guo Y, et,al. FIN56, a novel ferroptosis inducer, triggers lysosomal membrane permeabilization in a TFEB-dependent manner in glioblastoma. J Cancer. 2021 Sep 13;12(22):6610-6619. doi: 10.7150/jca.58500. PMID: 34659551; PMCID: PMC8517990. |
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| Cas No. | 1083162-61-1 | SDF | |
| Canonical SMILES | O=S(C1=CC(/C2=N\O)=C(C3=C2C=C(S(=O)(NC4CCCCC4)=O)C=C3)C=C1)(NC5CCCCC5)=O | ||
| 分子式 | C25H31N3O5S2 | 分子量 | 517.66 |
| 溶解度 | DMSO : ≥ 100 mg/mL (193.18 mM);Water : < 0.1 mg/mL (insoluble) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9318 mL | 9.6588 mL | 19.3177 mL |
| 5 mM | 0.3864 mL | 1.9318 mL | 3.8635 mL |
| 10 mM | 0.1932 mL | 0.9659 mL | 1.9318 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
