Fisetin-d5
目录号 : GC49344
An internal standard for the quantification of fisetin
Cas No.:2909407-29-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Fisetin-d5 is intended for use as an internal standard for the quantification of fisetin by GC- or LC-MS. Fisetin is a flavonoid that has been found in various fruits and vegetables and has diverse biological activities.1,2,3,4 It scavenges radicals in a Trolox equivalent antioxidant capacity (TEAC) assay and inhibits oxidative burst induced by N-Formyl-Met-Leu-Phe in isolated human polymorphonuclear neutrophils (PMNs) when used at a concentration of 10 µM.1,3 Fisetin (10-60 µM) induces cell cycle arrest at the G1 phase and apoptosis in, as well as inhibits the proliferation of, LNCaP prostate cancer cells.2 It inhibits LPS-induced production of nitrite, prostaglandin E2 , and NF-κB activation in RAW 264.7 cells.3 Fisetin (1 mg/animal) reduces tumor growth in a CWR22RΥ1 prostate cancer mouse xenograft model.4
1.Khan, N., Syed, D.N., Ahmad, N., et al.Fisetin: A dietary antioxidant for health promotionAntioxid. Redox Signal.19(2)151-162(2013) 2.Khan, N., Afaq, F., Syed, D.N., et al.Fisetin, a novel dietary flavonoid, causes apoptosis and cell cycle arrest in human prostate cancer LNCaP cellsCarcinogenesis29(5)1049-1056(2008) 3.Wang, L., Tu, Y.-C., Lian, T.-W., et al.Distinctive antioxidant and antiinflammatory effects of flavonolsJ. Agric. Food Chem.54(26)9798-9804(2006) 4.Khan, N., Asim, M., Afaq, F., et al.A novel dietary flavonoid fisetin inhibits androgen receptor signaling and tumor growth in athymic nude miceCancer Res.68(20)8555-8563(2008)
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4329 mL | 17.1644 mL | 34.3289 mL |
| 5 mM | 0.6866 mL | 3.4329 mL | 6.8658 mL |
| 10 mM | 0.3433 mL | 1.7164 mL | 3.4329 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。